Kazachstania paragamospora C. Y. Chai & F. L. Hui, 2024

Chai, Chunyue, Lu, Dan, Liu, Jinli, Wang, Eentao, Han, Xuemei & Hui, Fengli, 2024, Three novel Ascomycota (Saccharomycetes, Saccharomycetales) yeast species derived from the traditional Mexican alcoholic beverage Pulque, MycoKeys 109, pp. 187-206 : 187-206

Kazachstania paragamospora C. Y. Chai & F. L. Hui sp. nov.

Fig. 2 C

Etymology.

the specific epithet paragamospora , like gamospora , referring to its phylogenetic closeness to Kazachstania gamospora .

Type.

Mexico • State of Mexico, Teotihuacan , in the traditional Mexican alcoholic beverage Pulque, autumn of 2015, F. L. Hui, NYNU 161111 (holotype CICC 33274 T preserved in a metabolically inactive state, culture ex-type CBS 15233 ) .

Description.

After 3 days culture in YM broth at 25 ° C, the cells are spherical or ovoid (3–6.2 × 3.3–7.5 μm) and occurred singly or in pairs (Fig. 2 C ). Budding is multilateral. Sediment is formed after one month, but no pellicle is observed. After 3 days cultured on YM agar at 25 ° C, the streak culture is butyrous, white, raised with a smooth surface, and has a complete margin. Asci or signs of conjugation are not observed on sporulation media. Glucose, sucrose, raffinose, and inulin are fermented, while trehalose, galactose, maltose, melibiose, lactose, cellobiose, melezitose, methyl α - D-glucoside, soluble starch, or xylose are not. Glucose, sucrose (weakly), raffinose (weakly), inulin, glycerol (weakly), L-arabinitol (weakly), 5 - keto-D-gluconate (weakly), D-gluconate, D-glucosamine (weakly), succinate (weakly), methanol (weakly) and soluble starch (weakly) are assimilated. No growth occurred in the presence of melibiose, galactose, lactose, L-sorbose, L-rhamnose, L-arabinose, D-arabinose, D-ribose, ethanol, erythritol, galactitol, myo - inositol, DL-lactate, xylitol, D-glucuronate, D-glucono- 1, 5 - lactone, trehalose, maltose, melezitose, methyl α-D-glucoside, cellobiose, salicin, D-xylose, ribitol, mannitol, glucitol, citrate, 2 - keto-D-gluconate, or arbutin. With respect to the assimilation of nitrogen compounds, cadaverine (weakly) and D-tryptophan are assimilated, whereas L-lysine, nitrate, nitrite, ethylamine, creatine, creatinine, glucosamine, and imidazole are not. Growth is observed at 30 ° C but not at 35 ° C. Growth in the presence of vitamin-free medium is positive, but growth in the presence of 10 % NaCl plus 5 % glucose, 1 % acetic acid, 0.1 % cycloheximide, and 0.01 % cycloheximide are negative. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are also negative.

Additional strains examined.

Mexico • Teotihuacan in the State of Mexico (19°41'N, 98°50'W), in the traditional Mexican alcoholic beverage Pulque samples, autumn of 2015, F. L. Hui, NYNU 161114 and NYNU 161129 .

GenBank accession numbers.

Holotype CICC 33274 T (ITS: MF 136070 , D 1 / D 2: MF 136062 ) ; additional strains NYNU 161114 (ITS: OM 669944 , D 1 / D 2: OM 669945 ) and NYNU 161129 (ITS: OM 669946 , D 1 / D 2: OM 669947 ) .

Note.

Kazachstania paragamospora sp. nov. can be differed from the related three species K. gamospora , K. zonata and K. hellenica by its inability to assimilate trehalose (Table 2 ). Unlike K. hellenica , the novel species was able to ferment raffinose and grow at 30 ° C but was not able to ferment galactose. Likewise, the novel species did not assimilate maltose and D-galactose. The novel species differed from K. zonata by its inability to assimilate ethylamine, ethanol, and DL-lactate. It differed from K. gamospora in its ability to assimilate L-arabinitol and cadaverine (weakly), and inability to grow in the presence of 10 % NaCl plus 5 % glucose. In all cases, identification by sequencing was the best approach.

Figures

Figure 2. Morphological characteristics of Starmerella elongatum sp. nov. NYNU 161115 T A budding yeast cells, three days, YM broth, 25 ° C B pseudohyphae grown on CM agar after 14 d at 25 ° C. Kazachstania paragamospora sp. nov. NYNU 161111 T C budding yeast cells in YM broth after 3 d, 25 ° C. Pichia teotihuacanensis sp. nov. NYNU 161119 T D budding yeast cells, three days, YM broth, 25 ° C E simple pseudohyphae and F pseudohyphae grown on CM agar over 14 days at 25 ° C. Scale bars: 10 μm.