Pestalotiopsis hydei Huanraluek & Jayaward, 2021

Huanaluek, Naruemon, Jayawardena, Ruvishika S., Maharachchikum- Bura, Sajeewa S. N. & Harishchandra, Dulanjalee L., 2021, Additions to pestalotioid fungi in Thailand: Neopestalotiopsis hydeana sp. nov. and Pestalotiopsis hydei sp. nov., Phytotaxa 479 (1), pp. 23-43 : 35-39

Pestalotiopsis hydei Huanraluek & Jayaward sp. nov. ( Fig. 5 )

Index Fungorum number: IF558100; Facesoffungi number: FoF 09460

Etymology:— In honour of Assoc. Prof. Kevin D. Hyde.

Associated with leaf spot of Litsea petiolata . Conidiomata pycnidial in culture on PDA at 25 °C after 22 days, globose to oval, solitary or aggregated in clusters, semi-immersed, black, 195–890 μm diam., exuding globose, black, glistening conidial masses. Conidiophores indistinct, often reduced to conidiogenous cells. Conidiogenous cells discrete, fusiform, hyaline, cylindrical to subcylindrical or ampulliform to lageniform, rugose-walled, simple, 12–24 × 2–4 µm. Conidia fusoid, ellipsoid, straight to slightly curved, 4-septate, 18–35 × 3–6 µm; basal cell conic to obconic with a truncate base, hyaline, minutely verruculose, thin-walled, 3–7 µm long; three median cells doliiform, 12–18 µm long, wall minutely verruculose, pale brown, septa darker than rest of the cell (second cell from base 4–7 µm long; third cell 4–6 µm long, fourth cell 4–7 µm long); apical cell 3–12 μm long, hyaline, cylindrical to subcylindrical, thin- and smooth-walled; with 1–3 tubular apical appendages arising from the apical crest, unbranched, filiform, 3–12 μm long; basal appendage single, tubular, unbranched, centric, 2–8 μm long ( Fig 5 ).

Material examined:— THAILAND. Chiang Rai Province: Mae Fah Luang Botanical Garden, on leaf of Litsea petiolata Hook. f. ( Lauraceae ), 1 November 2018, Ruvishika S. Jayawardena and Naruemon Huanraluek BG 011 A (holotype MFLU 20–0470, ex-type MFLUCC 20–0135).

Notes:— Pestalotiopsis hydei found on a leaf of Litsea petiolata in Thailand, has similar morphological characters to P. grandis-urophylla and in the phylogenetic analyses our strain clustered with “ P. grandis-urophylla ” ( Fig. 2 ). However, when we checked for the morphological description for this species, we were unable to locate its complete description, type and ex-type details in the original publication. There is no record for type of the species name in Index Fungorum or Mycobank (2020). There are only 12bp differences between our strains of “ P. grandis-urophylla ”. Therefore, in this study we name the strains as P. hydei .

Figures

FIGURE 2. Consensus phylogram of 1,000 trees resulting from a RAxML analysis of the (ITS+TUB2+ Tef1) alignment of the analysed Pestalotiopsis sequences. RAxML bootstrap support values (ML above 60%), maximum parsimony (MP above 60%) and Bayesian inference (BYPP above 0.90) are given at the nodes (ML/MP/BYPP). The newly introduced sequences are in blue bold. The scale bar represents the expected number of changes per site. The tree is rooted to Neopestalotiopsis cubuna CBS 600.96 and Neopestalotiopsis saprophytica MFLUCC 12–0282.

FIGURE 5. Pestalotiopsis hydei. a. Colony top view. b. Colony reverse view. c. Conidiomata on PDA. d–e. Conidiogenous cells. f–k. Conidia. Scale bars d, e = 10 µm, f–k = 20 µm.