Hemiberlesia ozolita Schneider, Claps, Wei, Normark & Normark, 2020

Schneider, Scott A., Claps, Lucia E., Wei, Jiufeng, Normark, Roxanna D. & Normark, Benjamin B., 2020, Five new species of Aspidiotini (Hemiptera, Diaspididae, Aspidiotinae) from Argentina, with a key to Argentine species, ZooKeys 948, pp. 47-73 : 47

Hemiberlesia ozolita Schneider, Claps, Wei, Normark & Normark sp. nov. Figs 5 , 6

Material examined.

Holotype: Argentina • 1 adult female; Jujuy, Humahuaca, camino a Aparzo; 23.20S, 65.10W; 14.II.2002; L. E. Claps, P. Zamudio, L. Díaz-Briz, P. Cabrera leg.; IFML, L. E. Claps catalog #22-02, #1091 (D0288D). Paratypes: Argentina • 1 adult female; same slide and same data as holotype; IFML (D0288D) • 1 adult female; same data as holotype; USNM (D0288C) • 1 adult female; same data as holotype; USNM (D0288G) • 1 adult female; same data as holotype; UMEC (D0288F) • 1 adult female; same data as holotype; UMEC (D0288H) • 1 adult female; same data as holotype; UMEC (D0288I) • 1 adult female; same data as holotype; UMEC (D0288J).

Description

(N = 8). Adult female presumed to secrete scale cover, not pupillarial. Appearance in life not recorded. Slide-mounted adult female 770-1050 (median 910, holotype 990) μm long, 660-810 (median 780, holotype 810) μm wide; broadest at mesothorax. Body outline nearly circular; derm of prosoma becoming slightly sclerotized at full maturity (body length> 1mm), otherwise derm membranous except for pygidium. Antennae simple, each with one spine-like seta; distance between antennae 120-180 (median 140) μm. Without disc pores near anterior or posterior spiracles. Lobes: Only L1 well developed, apically truncate, with one deep lateral notch; L2 and L3 represented by small unsclerotized points. Paraphyses: Interlobular spaces between L1 and L2 and between L2 and L3 each with 2 clavate paraphyses; first pair similar in length to L1 and second pair nearly 1/2 that length. Plates: All plates rather simple, roughly triangular in shape with minimal fringing, and shorter in length than L1. One, minute, simple pair between L1; 2 present between L1 and L2, each with 1-2 short lateral fringes; 3 present between L2 and L3, simple or with one lateral fringe; plates absent anterior of seta marking position of L3. Ducts: Dorsal pygidial macroducts of uniform size; 1 marginal macroduct present between median lobes, 30-37 (median 34) μm in length, surpassing posterior margin of anal opening; 3-5 (median 3.5) macroducts arising from space between L1 and L2 (abdominal segment VII), 10-16 (median 14) on abdominal segment VI, 13-24 (median 17) on abdominal segment V, with a total of 31-42 (median 34) dorsal macroducts on each side of pygidium. Clusters of pre-pygidial macroducts present on dorsal submargins, 11-17 (median 13) on each side of abdominal segment IV, 10-16 (median 12) on segment III, 8-11 (median 10) on segment II, fewer present up to mesothorax. Ventral microducts few; present on head and thorax in submarginal and submedial rows; present in submargins of abdominal segments I-VI. Anal opening: Oval, maximum diameter (length) 14-23 (median 20) μm, situated 20-31 (median 25) μm, approximately 3 anal lengths, anterior to base of L1. Perivulvar pores : Absent.

DNA sequences.

DNA sequences of several loci of Hemiberlesia ozolita sp. nov. have been published from one paratype (D0288C) and one other individual from the type series that was ground to powder during the preparation of DNA (D0288A): 28S, GenBank accession numbers MH933989.1 (D0288C) and KY218997.1 (D0288A); EF-1α, MH915719.1 (D0288C) and KY221290.1 (D0288A); COI-II, MH916225.1 and MH916397.1 (D0288C), GQ425001.1 (D0288A); 16S of primary endosymbiont ( Uzinura diaspidicola ), KY220099.1.

Informal synonyms.

Specimens from the type series and their endosymbionts have appeared in several published phylogenetic trees, and have been referred to variously as " Diaspidiotus sp undesc #1" ( Morse and Normark 2006; Rugman-Jones et al. 2010), " Diaspidiotus sp nov 1" ( Andersen et al. 2010), and " Hemiberlesia ud0288" ( Schneider et al. 2018; Normark et al. 2019).

Remarks.

Hemiberlesia ozolita sp. nov. is most similar to H. nothofagi Williams, but H. ozolita sp. nov. is distinctive in having plates in the first space shorter than L1, 3 plates beyond L2, anal opening relatively small (<25 μm in diameter), 31-42 macroducts on each side of the pygidium, and groups of ventral submarginal microducts running from the pygidium to the thorax. In contrast, H. nothofagi has plates in the first space exceeding L1 in length, 6-7 plates beyond L2, a large anal opening (30 μm in diameter), about 26 pygidial macroducts per side, and few microducts present on the venter, not arranged in submarginal groups on thoracic and pre-pygidial segments. The new species is also similar to H. rapax except it has a much smaller anal opening and the pre-pygidial macroducts are longer, about as long as the pygidial macroducts.

Hemiberlesia ozolita sp. nov. constitutes the sister-lineage of a clade that includes all other sampled Hemiberlesia species and Palinaspis sordidata , according to the phylogenetic estimate of Schneider et al. (2018). The relatively small anal opening in this species is a trait shared in common with several other species formerly placed in Abgrallaspis that have since been transferred to Hemiberlesia ( Normark et al. 2014), a decision supported by molecular evidence.

Host plant.

Not recorded.

Etymology.

The specific epithet is an adjective formed from the Greek terms ozotos, meaning branching, and litos, meaning simple, and is used to describe the distinctly simple pygidial plates of this species.

Distribution.

Argentina (Jujuy).

Figures

Figure 5. Hemiberlesia ozolita sp. nov. Adult female, full body view, illustrated from a paratype (D 0288 C).

Figure 6. Hemiberlesia ozolita sp. nov. Adult female, expanded view of pygidium, illustrated from a paratype (D 0288 C).