Sugiyamaella cylindrica C.Y. Chai & F.L. Hui, 2022

Chai, Chun-Yue, Gao, Wan-Li, Yan, Zhen-Li & Hui, Feng-Li, 2022, Four new species of Trichomonascaceae (Saccharomycetales, Saccharomycetes) from Central China, MycoKeys 90, pp. 1-18 : 1

Sugiyamaella cylindrica C.Y. Chai & F.L. Hui sp. nov.

Fig. 3

Etymology.

The specific epithet cylindrica refers to the cylindrical vegetative cells of the type strain.

Type.

China, Henan Province, Luoyang City, Song County, the Tianchi Mountain National Forest Park , in rotting wood, October 2020, J.Z. Li & Z.T. Zhang (holotype NYNU 201067T, ex-type CBS 16662 = CICC 33514, holotype and ex-type are preserved in a metabolically inactive state) .

Description.

In YM broth after 3 days at 25 °C, cells are cylindrical (2-3 × 5-7 μm) and occur singly or in pairs. Budding is multilateral. Sediment is formed after a month, but a pellicle is not observed. On YM agar after 3 days at 25 °C, colonies are white to cream-coloured, butyrous, convex and smooth with entire margins. In Dalmau plate culture on corn meal agar, rudimentary pseudohyphae are formed. Asci or signs of conjugation are not observed on sporulation media. Glucose and trehalose are weakly fermented, but, galactose, maltose sucrose, melibiose, lactose, cellobiose, melezitose, raffinose, inulin and xylose are not fermented. Glucose, galactose, l-sorbose, glucosamine, d-ribose, d-xylose, l-arabinose, d-arabinose, l-rhamnose, sucrose, maltose, trehalose, methyl α-d-glucoside, cellobiose, salicin, melibiose, raffinose, melezitose, inulin, glycerol, erythritol, ribitol, d-glucitol, d-mannitol, galactitol, myo -inositol, d-glucono-1, 5-lactone, 2-keto-d-gluconate, 5-keto-d-gluconate, d-glucuronate, dl-lactate succinate, and ethanol are assimilated as sole carbon sources. Lactose, d-gluconate, citrate and methanol are not assimilated. Nitrate, nitrite, l-lysine, creatine, glucosamine, and d-tryptophan are assimilated as sole nitrogen sources. Ethylamine, cadaverine, creatinine, and imidazole are not assimilated. Minimum growth temperature is 15 °C, and maximum growth temperature is 35 °C. Growth in the presence of 0.1% cycloheximide is present, but growth in the presence of 1% acetic acid and 10% NaCl plus 5% glucose is absent. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.

Additional isolate examined.

China, Henan Province, Luoyang City, Song County, the Tianchi Mountain National Forest Park, in rotting wood, October 2020, J.Z. Li & Z.T. Zhang (NYNU 201034).

Notes.

Two strains were collected from two different substrates, representing Su. cylindrica , clustered in the Sugiyamaella clade and are closely related to Su. marilandica and Su. qingdaonensis . The nucleotide differences between the new species and the close relatives Su. marilandica and Su. qingdaonensis are 1.1-1.4% substitutions in the D1/D2 domain and 5.0-5.9% substitutions in the ITS region, respectively. Physiologically, Su. cylindrica differs from the closely related species Su. marilandica and Su. qingdaonensis ( Wang et al. 2010; Kurtzman 2011b) in its ability to assimilate glycerol and dl-lactate and to grow at 35 °C. Additionally, the new species ferments trehalose, while Su. marilandica and Su. qingdaonensis do not.

Figures

Figure 3. Morphology of Su. cylindrica (NYNU 201067, holotype) A budding cells were indicated by arrows in YM broth after 3 d B rudimentary pseudohyphae on CM agar after 14 d. Scale bars: 10 μm.