Sugiyamaella chuxiongensis C.Y. Chai & F.L. Hui, 2021

Shi, Cheng-Feng, Zhang, Kai-Hong, Chai, Chun-Yue, Yan, Zhen-Li & Hui, Feng-Li, 2021, Diversity of the genus Sugiyamaella and description of two new species from rotting wood in China, MycoKeys 77, pp. 27-39 : 27

Sugiyamaella chuxiongensis C.Y. Chai & F.L. Hui sp. nov. Figure 3

Type.

China, Yunnan Province, Chuxiong City, Zixi Town, in rotting wood from Zixi Mountain, August 2018, K.F. Liu & Z.W. Xi (holotype NYNU 181038T, culture ex-type CBS 16006, CICC 33361).

Description.

The cells are ovoid to elongate (2.5-4 × 3-4.5 μm) and occur singly or in pairs after growth in a YM broth for 3 days at 25 °C (Fig. 3A ). Budding is multilateral. After 3 days of growth on YM agar at 25 °C, the colonies are white to cream-coloured, buttery and smooth with entire margins. After 7 days at 25 °C, on a Dalmau plate culture with CM agar, hyphae and blastoconidia are formed (Fig. 3B ). Asci or signs of conjugation were not observed on sporulation media. Fermentation of sugars is absent. Glucose, galactose, l-sorbose, d-glucosamine, d-ribose, d-xylose, l-arabinose, d-arabinose, sucrose, maltose, trehalose, methyl α-d-glucoside, cellobiose, salicin, arbutin, melibiose, raffinose, melezitose, inulin, erythritol, ribitol, xylitol, d-glucitol, d-mannitol, d-glucitol, d-mannitol, galactitol, myo-inositol, 2-keto-d-gluconate, succinate, citrate and ethanol are assimilated. No growth was observed in l-rhamnose, lactose, glycerol, d-gluconate, dl-lactate or methanol. In nitrogen-assimilation tests, growth is present on nitrate, nitrite, ethylamine, cadaverine, creatine, creatinine, glucosamine and d-tryptophan, while growth is absent on l-lysine and imidazole. Growth was observed at 35 °C, but not at 37 °C. Growth in the presence of 0.1% cycloheximide, 10% NaCl with 5% glucose and 1% acetic acid is present, but growth in the presence of 16% NaCl with 5% glucose is absent. Starch-like compounds are not produced. Urease activity and diazonium blue B reactions are negative.

Additional isolates examined.

China, Yunnan Province, Chuxiong City, Zixi Town, in rotting wood from Zixi Mountain, August 2018, K.F. Liu & Z.W. Xi, NYNU 18521, NYNU 18634.

GenBank accession numbers.

holotype NYNU 181038T (ITS: MK682800; nrLSU D1/D2: MK682795); additional isolates NYNU 18521 (ITS: MT257260; nrLSU D1/D2: MT257255) and NYNU 18634 (ITS: MT257258; nrLSU D1/D2: MT257262).

Notes.

We generated sequences for three isolates of S. chuxiong , NYNU 18521, NYNU 181038 and NYNU 18634. This new species is phylogenetically most closely related to S. valenteae and S. ayubii (Fig. 1 ). Sugiyamaella chuxiong can be distinguished from S. valenteae , based on ITS and nrLSU D1/D2 loci (33/454 in ITS and 15/513 in nrLSU D1/D2) and from S. ayubii , based on ITS and nrLSU D1/D2 (42/499 in ITS and 35/565 in nrLSU D1/D2). Physiologically, S. chuxiong can be differentiated from S. valenteae by its ability to assimilate d-arabinose, sucrose, salicin, melibiose, raffinose, melezitose and inulin and its inability to ferment glucose and grow at 37 °C ( Sena et al. 2017). Similarly, the ability to assimilate salicin, inulin, erythritol and galactitol and the inability to assimilate l-rhamnose are the primary differences between S. chuxiong and S. ayubii . Additionally, S. ayubii can ferment glucose, while S. chuxiong cannot ( Sena et al. 2017).

Figures

Figure 1. Maximum Likelihood phylogenetic tree of Sugiyamaella inferred from the combined ITS and nrLSU dataset and rooted with Schizosaccharomyces pombe NRRL Y- 12796. The ML and MP bootstrap support values above 50 % are shown at the first and second positions, respectively. Newly-sequenced collections are in black boldface.

Figure 3. Morphology of S. chuxiongensis A budding cells after 3 days in YM broth at 25 ° C B hyphae and blastoconidia on corn-meal agar after 7 days at 25 ° C. Scale bars: 10 μm.