Stepanovia rosae Boyadzhiev & Todorov, 2013

Boyadzhiev, Peter S. & Todorov, Ivaylo A., 2013, Description of a new species of Stepanovia Kostjukov (Hymenoptera: Eulophidae) from Bulgaria, Revue suisse de Zoologie 120 (3), pp. 347-356 : 348-353

Stepanovia rosae Boyadzhiev & Todorov , sp. n.

Stepanovia sp. near eurytomae, Todorov et al. (2012) .

TYPE MATERIAL: Holotype: female: BULGARIA, VitoshaMt., near “Shevovitsa” area, 42°32’35”N / 23°20’54”E, 1249 m; 16.XI.2011; emerged 01-10.I.2012, fromgallsof Diplolepis rosae (Linnaeus, 1758) , on Rosa dumalis Bechstein 1810 ( MHNG). Paratypes: 2 females; VitoshaMt., “Shevovitsa” area, 42°32’08”N / 23°21’02”E, 1162 m; 16.XI.2011; emerged 01- 31. V.2012, from galls of D. rosae , on Rosa agrestis (Savi 1798) (MHNG) .- 30 females and 1 male; VitoshaMt., “Elovitsa” area, 42°29’31”N / 23°15’50”E, 1251 m; 30.X.2011; emerged 01- 31. V.2012, from galls of D. rosae , on R. agrestis (IBER) .- 31 females and 5 males; Vitosha Mt., “Elovitsa” area, 42°29’08”N / 23°16’07”E, 1133 m; 30.X.2011; emerged 01-31. V.2012, from galls of D. rosae , on Rosa micrantha Smith, 1812 ( MHNG).- 28 females and 2 males; Vitosha Mt., “Smilyo” area, 42°29’39”N / 23°15’41”E, 1277 m; 30.X.2011; emerged 01-31. V.2012, from galls of D. rosae , on Rosa canina Linnaeus, 1753 ( PUPB).- 2 females and 1 male; Vitosha Mt., “Smilyo” area, 42°29’38”N / 23°15’30”E, 1300 m; 30.X.2011; emerged 01-31. V.2012, fromgalls of D. rosae , on R. canina (IBER) .- 21 females and 1 male; Vitosha Mt., “Smilyo” area, 42°29’45”N / 23°14’47”E, 1335 m; 30.X.2011; emerged 01-31. V.2012, fromgallsof D. rosae , on R. canina (PUPB) .- 4 females; VitoshaMt., near “Terziyskidol” area, 42°33’26”N / 23°21’00”E, 1121 m; 16.XI.2011; emerged 01-31. V.2012, fromgallsof D. rosae , on R. dumalis (MHNG) .- 2 females, 2 males; VitoshaMt., near “Terziyskidol” area, 42°33’26”N / 23°21’00”E, 1121 m; 16.XI.2011; emerged 11-20.I.2012, fromgallsof D. rosae , on R. dumalis (PUPB) .- 1 male; VitoshaMt., near “Terziyskidol” area, 42°33’26”N / 23°21’00”E, 1121 m; 16.XI.2011; emerged 11-20.I.2012, fromgallsof D. rosae , on R. dumalis (ZISP) .- 1 female; VitoshaMt., near “Shevovitsa” area, 42°32’35”N / 23°20’54”E, 1249 m; 16.XI.2011; emerged 11-20.I.2012, from galls of D. rosae , on R. dumalis (ZISP) .- 1 female; Vitosha Mt., near “Shevovitsa” area, 42°32’35”N / 23°20’54”E, 1249 m; 16.XI.2011; emerged 01-10.I.2012, fromgallsof D. rosae , on R. dumalis (ZISP) .- 2 females; VitoshaMt., near “Dainkladenets” spring, 42°32’46”N / 23°13’56”E, 1374 m; 05.11.2011; emerged 01-31. V.2012, fromgallsof Diplolepisrosae (Linnaeus, 1758), on Rosacanina ( ZISP).

OTHER MATERIAL: 1 female; Vitosha Mt., “Elovitsa” area, 42°29’08”N / 23°16’07”E, 1133 m; 30.X.2011; emerged 01-31. V.2012, from galls of D. rosae , on R. micrantha Smith, 1812 ( PUPB).- 1 female, 1 male; Vitosha Mt., “Smilyo” area, 42°29’38”N / 23°15’33”E, 1286 m; 30.X.2011; emerged 01-31. V.2012, from galls of D. rosae , on R. canina (PUPB) .- 3 females; Vitosha Mt., near “Terziyski dol” area, 42°33’26”N / 23°21’00”E, 1121 m; 16.XI.2011; emerged 01-31. V.2012, from galls of D. rosae , on R. dumalis (PUPB) .- 3 females, 1 male; Vitosha Mt., near “Shevovitsa” area, 42°32’35”N / 23°20’54”E, 1249 m; 16.XI.2011; emerged 11-20.I.2012, from galls of D. rosae , on R. dumalis (PUPB) .

DIAGNOSIS: Close to Stepanovia eurytomae (Nees, 1834) but differing in having longer gaster and ovipositor (in rosae gaster 2.8-4 times as long as broad, ovipositor sheaths plus postcercale 0.66-0.69 length of hind tibia, sheaths 1.0-1.14 length of postcercale; in eurytomae gaster 2.0-2.6 times as long as broad, ovipositor sheaths plus postcercale 0.4-0.55 length of hind tibia, sheaths 0.5-0.8 length of postcercale); male antenna with ventral plaque situated about in the middle third of scape (in eurytomae situated hardly above middle of scape), body darker, head with U-shaped yellow stripe on lower face, gaster entirely brownish (in eurytomae head with at most mouth edge yellowish, gaster dorsally reddish at base, ventrally more or less yellowish).

Female: Head 1.1-1.33 times as broad as mesoscutum, 1.89-2.0 times as broad as long; temples 0.13-0.17 length of eyes; POL about 1.31-1.43 OOL, OOL about 2.0- 2.33 OD. Eyes about as long as broad, 1.23-1.31 times as high as wide, separated by 1.29-1.32 times their height and about 1.56 their length. Malar space approximately 0.75 length of eye and 0.57-0.63 height of eye, sulcus with triangular fovea extending about 0.4-0.42 length of gena. Mouth 1.33-1.5 malar space. Sides of face, near malar sulcus, with large punctures (Fig. 8). Vertex with numerous dark setae whose length is 0.52-0.68 about 0.7 OD. Head in front view 0.86-0.88 times as height as wide. Antenna (Fig. 1) with scape 3.13-3.5 as long as broad, 0.94-1.07 length of eye and 0.79-0.83 height of eye, not quite reaching median ocellus; pedicellus plus flagellum 1.18-1.29 breadth of mesoscutum; with four anelli (Fig. 2); pedicellus 1.81-1.84 times as long as broad and 0.79-0.82 as long as Fl; funicle proximally somewhat stouter than pedicellus, its segments decreasing in length, Fl 2.13-2.36 times, F2 about 1.56-0.69 times, F3 1.3-1.53 times as long as broad; clava broader than F3, 2.14-2.4 times as long as broad, about as long as F2 plus F3, with C1 as long as broad, C2 hardly shorter, C3 shorter than C2, spine about 0.33-0.4 length of C3 with apical seta about as long as spine; sensilla not numerous, irregularly biseriate, moderately long, decumbent. Thorax 1.35-1.44 times as long as broad; propodeal slope about 45°. Pronotum extremely short, 0.2 as long as mesoscutum, crescentic, with a row of setae near hind margin, these 0.71-0.83 as long as scutellar setae. Mid lobe of mesoscutum 0.85 as long as broad, convex and relatively dull; median line virtually absent, sometimes obscurely indicated by weaker reticulation of mesoscutum; 4 adnotaular setae on each side, hindmost about as long as first pair of scutellar setae. Scutellum 1.24-1.26 times as broad as long, about 0.77 as long as mesoscutum; submedian lines slightly nearer to sublateral lines than to each other, enclosing a space 2.4-2.6 times as long as broad; setae subequal, hindmost setae slightly greater than distance between submedian lines, anterior pair placed slightly before middle. Dorsellum 2.2 times as broad as long, hind edge curved; about 3.4 times shorter than scutellum. Propodeum medially about as Stepanovia rosae sp. n. (female). (1) Antennae. (2) Antennal anelli. (3) Forewing venation. (4) Hypopygium. (5) Genitalia. Scale lines = 0.01 mm for Fig. 2; for others = 0.1 mm.

long as dorsellum, moderately shiny, with fine, very slightly raised reticulation; median carina thin; callus with 2-3 setae. Legs of medium length; hind coxae oblique, about twice as long as broad, with hind edge curved; hind femora about 4.22 times as long as broad; spur of mid tibia about as long as or slightly shorter than basitarsus, fourth tarsomere 0.83-0.88 times shorter than basitarsus. Forewing (Figs 3, 10) about 2.29- 2.36 times as long as broad, reaching tip of postercale; costal cell shorter than M, 10 times as long as broad, distally with row of 4-5 setae on lower surface; SM with 3-4 dorsal setae; M rather thin, 4 times length of ST, its front edge with 12 setae; ST at about 45°, thin proximally and expanding a little distally to form poorly defined stigma; PM a distinct stub; speculum small, hardly extended below parastigma, closed below; wing beyond it moderately thickly pilose, more so distad; with extremely small bare spot just beyond stigma; cilia 0.25-0.29 length of ST. Hindwing (Fig. 6) narrowly rounded at apex; cilia 0.25-0.28 breadth of wing. Gaster (Figs 6, 9) lanceolate, acuminate, 2.0-2.15 times longer than thorax; 1.5-1.65 times longer than head plus thorax, about as broad as thorax, 2.8-4.0 times as long as broad; last tergite acuminate and forming a sublinear postcercale, 1.15-1.21 times as long as broad; ovipositor sheaths plus postcercale 0.66-0,69 length of hind tibia, sheaths 1.0-1,14 length of postcercale; longest seta of each cercus about 1.83-1.92 length of next longest, slightly sinuate or kinked; tip of hypopygium (Fig. 4) situated distinctly before half length of gaster. Genitalia (Fig. 5): outer plates of ovipositor (T 9 +T 10) 3.4 times as long as broad, 1.15 times as long as inner plates of ovipositor, and 1.7 times as long as ovipositor sheaths.

Colour: Head and thorax black with hardly perceptible metallic tinge; gaster entirely brownish; mouth edge, upper angle of mesopleuron, tegulae, dorsellum and antennae fuscous or brownish testaceous. Following parts yellow or fuscous: U-shaped stripe above clypeus to lateral parts of antennal torulli (Fig. 7), suture of frons just in front of median ocellus, mesothoracic spiracles, sometimes a small rhomboidal spot on lateral panel of pronotum above fore coxa, sutures of lateral part of mesothorax and distal sutures of scapular flange. Legs yellowish or very lightly fuscous with blackish coxae; dorsolateral surface of basal third of fore femur, dorsolateral surface of basal half of mid femur and up to two thirds of hind femur brownish; fore tarsi fuscous, fourth segment of mid and hind tarsi brownish or dark fuscous. Wings hyaline, venation yellowish. Length 1.48-2.2 mm.

Male: Differing from female as follows: POL 1.67 OOL. Antenna (Fig. 10) with scape 2.34-2.68 times as long as broad, its ventral plaque about 0.3 length of scape and placed about in the middle third; pedicellus plus flagellum 1.81 breadth of mesoscutum; with three anelli; pedicellus 1.33-1.75 as long as broad, slightly longer than Fl; funicle proximally as wide as pedicellus, filiform or tapering very slightly distad; Fl 0.63 as long as F2, 1.1 times as long as broad; F2 to F4 subequal in length, 1.83-2.0 as long as broad; clava 6 times as long as broad, 1.24 times longer than F3 plus F4; whorled setae moderately long, decreasing in length distally, those of Fl reaching tip of F3, that of C3 3.75 times longer than apical seta of terminal spine (Fig. 10, inset). Propodeum slightly longer than dorsellum. Gaster elliptic, 1.18 longer than thorax, 0.89 shorter than head plus thorax, 0.77 narrower than thorax, with ventral plica. Genitalia usually with two moderate-sized digital spines, but sometimes one digitus have three spines (Figs 12-13). Length 1.2-1.49 mm.

Stepanovia rosae sp. n. (female). (6) Lateral view of body (paratype). (7) Fronto-lateral view of head (paratype). (8) Punctures on lower face near malar sulcus (holotype). Scale lines = 0.1 mm. Stepanovia rosae sp. n. (9) Dorsal view of gaster (holotype). (10) Male antenna. (11) Male genitalia. (12) Paramere, digitus and aedeagus. Scale lines = 0.01 mm for Fig. 12 and for inset of Fig. 10; for others = 0.1 mm.

HOST: Probably Diplolepis rosae ( Hymenoptera , Cynipidae ). More detailed information about relationships on the gall community of D. rosae is given in Todorov et al. (2012).

ETYMOLOGY: Named after the host plant.