Sporothrix resoviensis R. Jankowiak & A. Ostafinska, 2021

Ostafinska, Agnieszka, Jankowiak, Robert, Bilanski, Piotr, Solheim, Halvor & Wingfield, Michael J., 2021, Six new species of Sporothrix from hardwood trees in Poland, MycoKeys 82, pp. 1-32 : 1

Sporothrix resoviensis R. Jankowiak & A. Ostafinska sp. nov.

Fig. 8

Etymology.

From Latin, referring to the capital of Podkarpackie Voivodeship (Resovia in Latin, Rzeszów in Polish), the region from which this fungus was collected.

Type.

Poland, Podkarpackie Province, Borownica , from the wound on Betula pendula , June 2016, A. Ostafińska, (O-F- 258632 holotype, culture ex-type CBS 147927) .

Description.

Sexual and asexual structures produced on sterilised birch twigs and on surface of malt agar in Petri dishes. Ascomata abundant, superficially or partly embedded in the agar, single or in groups; ascomatal bases black, globose, (87-)113-184(-232) μm diam., with brown hyphal hairs, 14 to 44 μm long and 0.9 to 2.2 μm wide at the base; ascomatal necks black, straight or curved, often extended at the base, (228-)378-624(-700) μm long, diameter (10-)11.2-17(-20.2) μm at the apex and (26.2-)34-47.7(-56) μm at the base. Ostiolar hyphae present, pale brown, septate, straight or curved, tapering towards the apex and often swollen at the tip, (7-)9-15(-18) in number, (15.7-)26.1-47.7(-67.6) μm long, (0.3-)0.7-1.5(-2.5) μm at the apex and (1.3-)2-3-(3.4) μm at the base. Asci evanescent. Ascospores one-celled, kidney-shaped to almost triangular in side view (2.7-)3.2-3.9(-4.4) × (1.4-)1.7-2.1(-2.3) μm, oblong-elliptical in front view (2.6-)3-3.8(-4.9) × (1.4-)1.7-2.2(-2.6) μm, without residual sheath accumulated in white-colored mass at the tip of the neck. Conidiophores hyaline, micronematous, straight, simple and bearing several conidiogenous cells, either borne on vegetative hyphae or on upright hyphae. Conidiogenous cells blastic, cylindrical, terminal, lateral or intercalary, straight or curved, swollen apical part forming conidia by sympodial proliferation on easily visible denticles, (3.1-)9.3-57(-120.1) μm long, (1-)1.1-1.6(-2.2) μm wide at the base. Apical part (1.3-)1.9-3.5(-4.4) μm long and (1.4-)2.4-3.9(-4.5) μm wide. Conidia hyaline, unicellular, smooth, obovate to ellipsoidal, pointed at the base, (3.9-)4.3-6.7(-8.5) × (2.1-)2.4-3.4(-4) μm, formed singly on denticles or on the side of vegetative hyphae. Culture characteristics: Cultures showing optimum growth at 25 °C (1.8 mm/d) followed by at 30 °C (1.7 mm/d), white, growing in a circular pattern with smooth margins, funiculose and woolly.

Host trees.

Betula pendula .

Insect vector.

unknown.

Distribution.

Poland.

Note.

Sporothrix resoviensis is phylogenetically distinct from the other Sporothrix species based on the ITS, βT, CAL and TEF1-α sequences. This species grouped most closely with S. stenoceras but can be distinguished by its larger ascospores ( S. resoviensis : 2.7-4.4 × 1.4-3.3 μm; S. stenoceras : 2.0-2.9 × 1.3-1.4 μm ( Robak 1932). Perithecia developing on the agar medium and twigs have significantly shorter necks ( S. resoviensis : 228-700 μm; S. stenoceras : 450-1500 μm ( Robak 1932). Sporothrix resoviensis has larger conidia (3.9-8.5 × 2.1-4 μm) compared to S. stenoceras (3.4-6.9 × 2-3.4 μm). This new species also differs from S. stenoceras based on culture morphology, where S. resoviensis produces wooly cultures, different to the sparse and flat mycelium of S. stenoceras ( Robak 1932).

Sporothrix resoviensis was represented by one isolate collected from a wound on Betula pendula . It corresponds to Sporothrix sp. 10 in the study of Jankowiak et al. (2019b).

Figures

Figure 8. Sporothrix resoviensis sp. nov. (CBS 147927) a ascoma b, c ostiolar hyphae d ascospores e-g conidiogenous cell with an inflated cluster of denticles at the apex h conidia i fourteen-day-old culture on MEA. Scale bars: 250 μm (a), 25 μm (b, c), 10 μm (d), 25 μm (e), 10 μm (f-h).