Smittina imragueni, Matsuyama, Kei, Titschack, Jürgen, Baum, Daniel & Freiwald, André, 2015

Matsuyama, Kei, Titschack, Jürgen, Baum, Daniel & Freiwald, André, 2015, Two new species of erect Bryozoa (Gymnolaemata: Cheilostomata) and the application of non-destructive imaging methods for quantitative taxonomy, Zootaxa 4020 (1), pp. 81-100 : 89-95

Smittina imragueni n. sp.

( Figs 6 , 7 )

Material examined. Holotype: SMF 40005, station 14904-1, 17°32.559ʹ N, 16°39.805ʹ W, 510 m, Tamxat Mound Complex. Paratypes: SMF 40006, station 14904-1, 17°32.559ʹ N, 16°39.805ʹ W, 510 m, Tamxat Mound Complex; SMF 40007, station 14904-1, 17°32.559ʹ N, 16°39.805ʹ W, 510 m, Tamxat Mound Complex; SMF 40008, station 14904-1, 17°32.559ʹ N, 16°39.805ʹ W, 510 m, Tamxat Mound Complex; SMF 40009, station 14904-1, 17°32.559ʹ N, 16°39.805ʹ W, 510 m, Tamxat Mound Complex; SMF 40010, station GeoB 14911-1, 17°28.910ʹ N, 16°41.509ʹ W, 450 m, Tamxat Mound Complex. Smittina cervicornis : NHMUK 1911.10.1.1428, Adriatic, 34 m; NHMUK 1887.12.9.585, Cape Verde Islands, 183–274 m; NHMUK 1944.1.8.281, Cape Verde Islands, 183–219 m; SMF 9811, Riou Island, 34 m; SMF 40011, Adriatic, 15 m.

Etymology. The species name is derived from the Imraguen, a tribe of Mauritanian fishermen inhabiting the area around Banc d’Arguin.

Diagnosis. Erect, bilaminar Smittina with an oval suboral avicularium on the lyrula within the peristome, orientated longitudinally, almost normal to the direction of growth. Spines absent.

Description. Colony erect, bilaminar, multiserial. Dichotomously branching, internodes flattened ( Fig. 6 A). The laminae of each branch are symmetrical to each other with respect to autozooidal arrangement. Autozooids arranged in diagonal series consisting of four autozooids ( Fig. 7 A). Neighbouring zooids on narrow side of branch are on same level ( Fig. 7 B). Zooids more or less rectangular, distal end rounded, surface convex, boundaries distinct. Sutures in primary calcification straight in cross-sections; serrated in area of secondary calcification ( Fig. 6 B). Septular pores simple. Frontal shield cryptocystidean, evenly porous. Primary orifice elongate-semicircular, deeply immersed, lyrula about half as wide as orifice, truncate. Condyles rounded, directed proximomedially. Oral spines absent ( Fig. 6 C–E). Proximally, but still on lyrula, distinct oval avicularium, nearly normal to direction of growth. Rostrum oval, rounded, proximal border finely serrate, crossbar complete, with bifid ligula ( Fig. 6 C–F). Thick cylindrical peristome develops through secondary calcification, projecting from colony surface, almost concealing primary orifice and in later ontogeny incorporating and almost concealing avicularium. Secondary calcification leads eventually to flattened colony surface, owing to immersion of peristomes and avicularia; peristomial rim forms circular or oval secondary orifice that eventually becomes constricted in middle by ‘pseudocondyles’, separating avicularium from orifice and resulting in keyhole shape ( Fig. 6 F). Zooids early in astogeny form encrusting base ( Fig. 6 G, H); these zooids have lyrula with median ridge, and peristomes sometimes closed by secondary calcification during ontogeny, leading to bulbous appearance ( Fig. 6 H). Ancestrula same shape as autozooids, but smaller and with fewer pores ( Fig. 6 G). Ovicells not observed.

Micro-CT imaging and quantification. Interior zooidal chamber larger transversely than basal-frontally ( Fig. 7 A, B). Avicularium connected mediofrontally to zooidal chamber, immediately proximal to orifice ( Fig. 7 C, D); avicularian chamber shaped like rugby ball, orientated transversely and perpendicular to rostrum, and tapering laterally around zooidal chamber. Two or three frontal pores arise from avicularian chamber, indistinguishable from other frontal pores originating from autozooidal chamber ( Fig. 7 E). Frontal pores cylindrical ( Fig. 7 C, D).

Values for the volume and the maximum extent of all three spatial axes of the zooidal and avicularian chambers clearly show two distinct groups (n = 16) ( Fig. 8 ). The variance for both groups differs depending on the character measured. Zooids show the smallest variation in the width of the zooidal chamber ( Table 6 ), whereas avicularia show the smallest variance in depth ( Table 7 ).

Orificial and frontal-pore measurements form three clusters ( Figs 7 F, 9): a large cluster of 441 data points plots between 20 and 50 µm; a second group of 18 data points between 180 and 240 µm, with a mean of 209 µm; and a third group of 56 data points ranging in size from 50 to 140 µm. This last group is most evident in the length plot ( Fig. 9 A), while in the width plot separation from the second group is less distinct ( Fig. 9 B).

Difficulties in species identification arise from intracolony variation owing to increasing calcification during ontogeny. While peristomes and avicularia are easily discernible in younger parts of the colony, they become scarcely discernible in older parts. The zooidal boundaries also become more irregular and partly occluded. These limitations can be overcome by using micro-CT imaging techniques or by viewing distal branch segments in the early ontogenic stage. As the internal basal and lateral walls were not completely resolved during the scan, owing to comparatively low acceleration voltage resulting in only a fragmentary reconstruction, we cannot describe the distribution and shape of communication pores. It is evident from the reconstructed transverse stack that new zooids originate from the median lamina, simultaneously on each side.

Remarks. Smittina imragueni n. sp. closely resembles S. cervicornis ( Pallas, 1766) . Smittina cervicornis is one of the bryozoans mentioned earliest in the literature, as references to this species can be traced back to Imperato (1599), who described and figured an erect, laminar, branching species from the Mediterranean as “ Porus cervinus ”. However, confusion exists concerning the actual type, as Pallas’s collection appears to be lost ( Kuklinski & Taylor 2008) and no lectotype has been designated. Pallas also did not provide any figures.

Smittina imragueni n. sp. differs from an Adriatic specimen of S. cervicornis (NHM 1911.10.1.1428) in the position of the avicularium. In the Adriatic colony, it is positioned in the same plane as the frontal surface, whereas in S. imragueni it extends from the lyrula along the proximal peristomial margin, resulting in a more oblique orientation relative to the direction of colony growth. The distal end of the rostrum is also more acute in the Adriatic specimen. It is noteworthy that this Adriatic specimen, which is part of the Canon Alfred Merle Norman collection, has a type label that is amended by “?part of type material”. A small included note states that it was originally described as “ Porella tubulifera Heller ” and was apparently sent by Camil Heller. Another species from the Adriatic (SMF 40011) has the avicularium oriented 35° to 45° from the direction of zooidal growth, which still appears less oblique than in S. imragueni . The avicularium itself also has an acute rostrum. Material from the Adriatic figured by Hayward & McKinney (2002) as Smittina cervicornis seems to be this species. A western Mediterranean specimen from Riou Island (SMF 9811) has the same more-frontal orientation of the avicularium as the Adriatic specimens, but the shape of the rostrum appears similar to that in S. imragueni , though it is even more acute. At present, it is uncertain whether the Riou Island specimen is conspecific with Adriatic S. cervicornis . Two other specimens at the NHM, from the Cape Verde Islands, also recorded as S. cervicornis , which were originally described as Porella laevis var. subcompressa Busk, 1884 (NHM 1887.12.9.585 and NHM 1944.1.8.281), show the same orientation of the avicularium as the Mediterranean species, but differ from S. imragueni and S. cervicornis in other aspects of zooid morphology and in having differently shaped and also dimorphic suboral avicularia.

Another similar species is the less well-known Smittina colletti ( Jullien & Calvet, 1903) , which differs from S. imragueni in the orientation and the relative size of the suboral avicularium and S. colletti also has a characteristic median denticle on top of the lyrula. The zooidal sizes given by Harmelin (1969) for S. colletti are significantly smaller than those for S. imragueni .

Strong secondary calcification and intracolony variation complicate identification for these three species, and close attention should be given to the shape and orientation of the suboral avicularium. Further studies might reveal the true identity of Smittina cervicornis s.s.

Distribution. Smittina cervicornis occurs mainly in the Mediterranean ( Zabala & Maluquer 1988; Rosso 2003), but it has also been reported along the West African coast, from Morocco ( Canu & Bassler 1925, 1928; d’Hondt, 1978) to Equatorial Guinea ( Cook 1968, 1985) and the Canaries ( Aristegui & Cruz 1986). Its northern extent is the coast of Brittany ( Harmelin et al. 1994). The bathymetric range seems to be from shallow coastal water down to 120 m ( Cook 1968; Harmelin et al. 1994; Hayward & McKinney 2002), but Canu & Bassler (1928) reported a depth of 400 m in the Bay of Biscay. Smittina colletti has also been reported from the Bay of Biscay ( Jullien & Calvet 1903; Harmelin 1969) and the Western Mediterranean ( Gautier 1962; Harmelin 1969), in waters deeper than 100 m. The short account of the different forms of “ Smittina cervicornis ” given above suggests that this is in fact a species complex, containing at least three species. Smittina imragueni n. sp. is so far known only from cold-water coral and adjacent habitats off Mauritania, at depths of 414 to 600 m, where it forms small, branched colonies attached to the dead coral framework in the basal part of small patches of living and dead colonies of the corals Lophelia pertusa ( Linnaeus, 1758) and Madrepora oculata ( Linnaeus, 1758) . In the area investigated, S. imragueni occurred south of 18.5° N and in the Arguin Canyon ( Table 1 ; Fig. 1 ).

Figures

FIGURE 1. Charts showing the positions of all stations investigated based on MSM 16 - 3 multibeam surveys. Stations in bold font are those where either C. bafouri n. sp. or S. imragueni n. sp. occurred. Black areas in the insets A – H denote data gaps. The bathymetry of the overview map is from CleanTOPO 2, http: // www. shadedrelief. com / cleantopo 2 /.

FIGURE 6. SEM micrographs of Smittina imragueni n. sp. A, aspect of colony fragments (right, holotype SMF 40005; left, paratype SMF 40006). B, cross-section through a branch, showing thick secondary calcification and different profiles of zooidal chambers (paratype SMF 40007). C, interior view of primary orifice and suboral avicularium (SMF 40007). D, autozooids showing a relatively early developmental stage of the peristome (SMF 40010). E, secondary orifice with an intermediately developed peristome (SMF 40006). F, immersed secondary orifice late in ontogeny; note the vertical orientation of the avicularium (SMF 40007). G, ancestrular area (paratype SMF 40008). H, transition between encrusting and erect growth (paratype SMF 40009). Scalebars: A, 2 mm; B, G, 500 µm; C, E, 100 µm; D, F, H, 200 Μm.

FIGURE 7. Virtual reconstructions of the internal structure of Smittina imragueni n. sp. (paratype SMF 40010). A, broad side of a branch. B, narrow side of a branch. C, single autozooid with suboral avicularium. D, same as C, without the suboral avicularium. E, a single suboral avicularian chamber; the two frontal protuberances are frontal pores. F, automatically detected orifices and frontal pores. Scalebars: A, B, F, 500 µm; C, D, 250 Μm; E, 50 Μm.

FIGURE 8. Scatter plots showing the volume (A), length (B), width (C) and depth (D) of zooidal and avicularian chambers automatically detected and measured on the virtual dataset (N = 16) for Smittina imragueni n. sp. As in Fig. 4, the horizontal axis represents an arbitrary numbering by the software Amira. A separation between zooidal (large values on the left) and avicularian chambers (small values on the right) is clearly visible.

FIGURE 9. Scatter plots showing length (A) and width (B) of orifices and frontal pores automatically detected and measured on the virtual dataset (N = 503) for Smittina imragueni, showing a distinction between those two entities, with some datapoints plotting in between (see Discussion). As in Fig. 4, the horizontal axis represents an arbitrary numbering by the software Amira.

Tables

TABLE 6. Measurements (in µm) of internal zooidal chambers for Smittina imragueni n. sp. (paratype, 40010), made from the virtual dataset.

Autozooid Volume (10 9 µm3)	Length (µm)	Width (µm)	Depth (µm)
1 0.070	1240	538	572
2 0.068	1237	579	569
3 0.092	1223	529	628
4 0.079	1185	439	637
5 0.082	1177	480	611
6 0.089	1168	489	578
7 0.069	1134	524	645
8 0.062	1080	493	565
Mean 0.076	1181	509	601
SD 0.010	51	40	31

TABLE 7. Measurements of avicularian chambers for Smittina imragueni n. sp. (paratype, 40010), made from the virtual dataset.

Avicularium Volume (10 9 µm³)	Width (µm)	Length (µm)	Depth (µm)
1 0.003	377	174	166
2 0.002	359	248	156
3 0.003	306	279	166
4 0.003	301	280	162
5 0.003	337	326	145
6 0.003	296	350	176
7 0.002	314	201	167
8 0.003	317	248	179
Mean 0.003	326	263	165
SD 0.0005	27	55	10