Pseudamnicola (Corrosella) hauffei, Delicado, Diana & Ramos, Marian A., 2012

Delicado, Diana & Ramos, Marian A., 2012, Morphological and molecular evidence for cryptic species of springsnails [genus Pseudamnicola (Corrosella) (Mollusca, Caenogastropoda, Hydrobiidae)], ZooKeys 190, pp. 55-79 : 63-67

Pseudamnicola (Corrosella) hauffei sp. n.

Type locality.

Los Nogales spring, Benafer, Castellón, Spain, 30°55.80'N, 0°34.34'W.

Type material.

Holotype MNCN 15.05/60026a (SEM preparation, Figure 5A) and paratypes (Figures 5 D–G, 6, 7) MNCN 15.05/60026b (SEM preparation, Figures 5 D–G, 6, and 70° ethanol, Figure 7) and MNCN/ADN 54952-54969 (frozen material and 70° ethanol), D.D. & C.N., 19 March 2009; MNCN 15.05/60027 (70° ethanol), 26 May 1998, B.A.

Material examined.

Four males and four females from type locality were examined for anatomical study. In addition, some populations from provinces of Castellón and Valencia (Spain) were also found and studied, dissecting likewise two males and two females from each for their identification.

Localities.

Los Nogales spring, Benafer, Castellón, Spain (type locality), 30°55.80'N, 0°34.34'W, B.A., 26 May 1998, MNCN 15.05/60027 (70° ethanol); D.D. & C.N., 19 March 2009, MNCN 15.05/60026 (70° ethanol and ESEM preparation, Figures 5A, D–G) and MNCN/ADN 54952-54969 (frozen material); Agadín spring, Benafer, Castellón, Spain, 39°56.38'N, 0°34.54'W, D.D. & C.N., 19 March 2009, MNCN 15.05/60028 (70° ethanol and ESEM preparation, Figure 5C) and MNCN/ADN 54970-54974 (frozen material); irrigation ditch in Navajas, Castellón, Spain, 39°52.09'N, 0°30.37'W, R.A., D.M. & J.M.R 7 March 1990, MNCN 15.05/60029 (70° ethanol); Curso spring, Navajas, Castellón, Spain, 39°52.43'N, 0°30'W, B.A., 25 May 1998, MNCN 15.05/60030 (70° ethanol) and MNCN/ADN 54975-54989 (frozen material); La Peña spring, Navajas, Castellón, Spain, 39°52.77'N, 0°30.03'W, R.A., D.M. & J.M.R, 7 March 1990, MNCN 15.05/60031 (70° ethanol); La Esperanza spring, Navajas, Castellón, Spain, 39°52.19'N, 0°30.43'W, R.A., D.M. & J.M.R, 7 March 1990, MNCN 15.05/60032 (70° ethanol); Del Prado spring, Viver, Castellón, Spain, 39°56.23'N, 0°36.81'W, D.D. & C.N., 19 March 2009, MNCN 15.05/60033 (70° ethanol) and MNCN/ADN 54990-54992 (frozen material); San Miguel spring, Viver, Castellón, Spain, 39°55.68'N, 0°36.64'W, B.A., 25 May 1998, MNCN 15.05/60034 (70° ethanol); D.D. & C.N., 19 March 2009, MNCN 15.05/60035 (70° ethanol and ESEM preparation, Figure 5B) and MNCN/ADN 54997-54999 (70° ethanol); San Miguel ditch, Viver, Castellón, Spain, 39°55.68'N, 0°36.64'W, D.D. & C.N., 19 March 2009, MNCN 15.05/60036 (70° ethanol) and MNCN/ADN 54993-54996 (frozen material); Font Nova, Benifaió, Valencia, Spain, 39°0.55'N, 0°5.87'W, B.A., 26 May 1998, MNCN 15.05/60037 (70° ethanol); Cortés de Pallás, Valencia, Spain, 39°14.61'N, 0°26.01'W, B.A., 26 May 1998, MNCN 15.05/60038 (70° ethanol).

Material examined for morphometry.

Shell, anatomical, operculum and radular measurements (Tables 2-7) were made on specimens from the type locality, Los Nogales spring in Benafer, Castellón.

Etymology.

Dedicated to the malacologist and ecologist Torsten Hauffe, for his help and support during the stay of the first author in Germany.

Diagnosis.

Shell yellowish with body whorl occupying 2/3 shell length; umbilicus slightly visible; protoconch microsculpture grooved; central radular tooth formula 5-C-5; style sac protruding below non-pigmented intestine; elongate bursa copulatrix J-shaped; renal oviduct pigmented until seminal receptacle, which has a pigmented short duct; penis triangular with a wide base attached to central area of head; nervous system brown pigmented with supraoesophageal connective about three times longer than suboesophageal.

Description.

Shellovate-conic (Figure 5 A–C), yellowish periostracum with 4-4.5 spire whorls, height around 2.0-3.0 mm (Table 2); protoconch approximately 450 µm wide with 1.5 whorls and a nucleus around 200 µm long (Figure 5E, F); protoconch microsculpture grooved (Figure 5G); body whorl about 2/3 total length; whorls convex with deep suture; peristome frontal, complete, oval, with thick inner lip partly hiding umbilicus; outer peristome simple, straight (Figure 5D).

Operculum corneous, yellowish, thin, pliable, ellipsoidal, paucispiral, with nucleus submarginal (Figure 6A, B; Table 3); oval muscle attachment near nucleus.

Radula with around 50 rows of teeth, medium in size (25% total shell length) (Figure 6C, Table 4); central tooth with a tongue-shaped median cusp and five lateral cusps, slightly sharpening towards central one (Figure 6D, E); lateral teeth with a long tongue-shaped median cusp and three tapered laterals; inner and outer marginal teeth bear 15 and 19 sharp cusps respectively (Figures 6D, F).

Pigmentation and anatomy: Head intensely brown pigmented from snout to neck (Figure 7D); pigment on neck clearer than on head; brown band of pigment also on tentacles, but not on ocular lobes; snout as long as wide, with medial lobation; foot intermediate length, pigmented on dorsal region. Ctenidium in the anterior region of pallial cavity with about 15 gill filaments; osphradium ellipsoidal under central gill filaments (Figure 7C, Table 5). Stomach slightly longer than wide (Figure 7F); style sac barely shorter than stomach, protruding below intestine (Table 5).

Female genitalia with a pallial oviduct about four times longer than wide (Figure 7G; Table 6); capsule gland slightly longer than albumen gland and denser in posterior region; genital aperture in the anterior extreme of pallial oviduct; elongate bursa copulatrix, J-shaped folded with a duct less than 50% bursa length; renal oviduct scarcely pigmented from the insertion point of bursal duct to where it begins to fold and black pigmented, making two or three loops; elongate seminal receptacle with pigmented short duct (Figure 7H) joining renal oviduct slightly above the point where the bursal duct joins the renal oviduct.

Male genitalia bearing a bean-like prostate gland about three times longer than wide (Figure 7E, Table 6); penis triangular with a wide base attached to central area of head with some folds in middle section and a narrow patch of black pigment on distal surface (Figure 7D); vas deferens uncoiled in penis running straight close to the external margin.

Nervous system brown pigmented, but ganglia darker than connectives and commissures; cerebral ganglia equal in size; supraoesophageal and suboesophageal ganglia similar in shape and size; supraoesophageal connective around three times longer than suboesophageal (Figure 7A, B; Table 7). Mean RPG ratio 0.51 (elongated).

Remarks.

Some of the localities where this species was found were cited by Gasull (1981) incorrectly as inhabited by Pseudamnicola (Corrosella) astieri . Both species show marked differences such as: 1) Pseudamnicola (Corrosella) astieri has a longer shell, longer spire (SL-LBW) (Table 2) and the protoconch microsculpture is more granulated than in Pseudamnicola (Corrosella) hauffei sp. n. Moreover, the inner lip of the shell aperture in Pseudamnicola (Corrosella) hauffei sp. n. is thicker than in Pseudamnicola (Corrosella) astieri and partly hides the umbilicus; 2) central radular tooth with seven lateral cusps in Pseudamnicola (Corrosella) astieri , five in Pseudamnicola (Corrosella) hauffei sp. n. (Table 4); 3) style sac surrounded by black pigmented intestine in Pseudamnicola (Corrosella) astieri yet lacks pigment and protrudes under the intestine in Pseudamnicola (Corrosella) hauffei sp. n. (Figures 4F and 7F); 4) bursa copulatrix J-shaped and seminal receptacle with a short duct in Pseudamnicola (Corrosella) hauffei sp. n., while bursa copulatrix is U-shaped and seminal receptacle is shorter and lacks a duct in Pseudamnicola (Corrosella) astieri (Figures 4H and 7H); 5) penis triangular with a wide base in Pseudamnicola (Corrosella) hauffei sp. n. and slender in Pseudamnicola (Corrosella) astieri (Figures 4D and 7D); 6) nervous system elongated (RPG= 0.51) in Pseudamnicola (Corrosella) hauffei sp. n. yet moderately concentrated (RPG= 0.42) in Pseudamnicola (Corrosella) astieri .

Compared to the other Pseudamnicola (Corrosella) species living in nearby areas, Pseudamnicola (Corrosella) hinzi and Pseudamnicola (Corrosella) navasiana , Pseudamnicola (Corrosella) hauffei sp. n. has a shorter and more ovate shell shape, a longer bursa copulatrix, bursa duct and seminal receptacle, and a more triangular wider-based penis.