Pista anneae, Nogueira, João Miguel Matos, Hutchings, Pat & Carrerette, Orlemir, 2015

Nogueira, João Miguel Matos, Hutchings, Pat & Carrerette, Orlemir, 2015, Terebellidae (Annelida, Terebelliformia) from Lizard Island, Great Barrier Reef, Australia, Zootaxa 4019 (1), pp. 484-576 : 510-516

Pista anneae n. sp.

( Figs 1 D, 16–20)

Type material. Holotype: AM W.44513, MI QLD 2370, Vicki´s Reef, 14°41'16"S, 145°26'35"E. Paratypes: AM W.44958, MI QLD. 2424 (mounted on SEM pin), reef on north eastern side of South Island, 14°42'13"S, 145°27'37"E, 21 Aug 2013; AM W.47711, CReefs, LI–10–041, MI QLD 2202, MacGillivray Reef, 14°39'24"S, 145°29'34"E, complete, in excellent state, 92 segments, ~ 18 mm long, ~ 1 mm wide.

Comparative material examined. Holotype of Pista curtiuncinata Hartmann-Schröder, 1981 , HZM P16500. Holotype of Pista kimberliensis Hutchings & Glasby, 1990 , AM W.203525. Holotype of Pista pectinata Hutchings, 1977 , AM W.6795. Holotype of Pista pegma Hutchings & Smith, 1997 , AM W.22574. Holotype of Pista trina Hutchings, 1977 , AM W.6798. Holotype of Pista trunca Hutchings, 1977 , AM W.6973. Holotype of Pista typha Grube, 1878 , MPW 518. Holotype of Pista violacea Hartmann-Schröder, 1984 , NTM W.1644.

Description. In life, orange to red anterior body with bright red branchiae, brownish after termination of notopodia, colourless buccal tentacles ( Fig. 1 D). Transverse prostomium attached to dorsal surface of upper lip; basal part with eyespots in thin, continuous row, mid-dorsal gap absent; distal part shelf-like. Relatively elongate buccal tentacles lacking any pigmentation, longer tentacles longer than region with notopodia ( Figs 1 D; 16A–I, K– M, O –Q, 17A–M, 18A). Peristomium forming lips, hood-like upper lip, short, wider than long; short and swollen lower lip, button-like ( Figs 16 A, F, I, P; 17B, G–H, M). Segment 1 narrow, forming low ventral lobe marginal to mouth; anterior segments with thickened anterior margins dorsally, forming low crests; segment 2 also protruding as a ventral crest, with pair of triangular, distally rounded ventro-lateral lobes; segment 3 with pair of developed lateral lobes, larger than those segment 2 and covering them, with straight lateral and distal margins; segment 4 with pair of much shorter rounded lobes, almost inconspicuous ( Figs 16 A–I, K–M, O –Q; 17A–M; 18A). Anterior segments not remarkably inflated dorsally. Paired dorso-lateral plumous branchiae present on segments 2–3, usually first pair distinctly larger and inserted more dorsally, but frequently missing one or both branchiae of a pair, and different sizes within a pair; each branchia with relatively long, crenulate basal stem, and branchial filaments originating in a spiral at tip; branchial filaments dichotomously branching for few levels, with lateral ciliary track and short tips ( Figs 16 B– E, G–H, K–M, O, Q; 17A, C–F, I–L; 18A–C). Smooth, slightly corrugated anteriorly mid-ventral shields present from segment 2 to termination of notopodia, on segment 20, rectangular shields, of uniform width except for last 3– 4 pairs ( Figs 16 C, E–F, I, L, O –P; 17B, G–J, L–M). Notopodia beginning on segment 4, extending until segment 20; notopodia short, rectangular, all longitudinally aligned, first two pairs slightly shorter and dorsally aligned to following pairs ( Figs 16 A–E, G–H, K–M, O, Q; 17A–M; 18A–B, D–F, J–K). Broadly-winged notochaetae in both rows, rounded geniculate wings, broader on one side, those from posterior row with wings at tips

only ( Figs 18 E–G, J–K; 19A–F). Neuropodia present from segment 5, as low, almost sessile ridges until termination of notopodia, as low rectangular pinnules thereafter ( Figs 16 A–G, I–L, N–Q; 17A–C, G–M). Neurochaetae as long-handled avicular uncini on segments 5–10, thin handles originating from heel only, progressively less developed; on segments 11–20, short-handled uncini, arranged in completely intercalated double rows; avicular uncini throughout, with short, triangular and distally rounded heel, rounded prow, dorsal button at mid-length, conspicuous throughout, and crest with 4–5 transverse rows of numerous, progressively shorter secondary teeth ( Figs 19 G–L; 20A–G). Nephridial papillae at base of branchiae of segment 3, genital papillae on segments 6–7, dorsal and posterior to notopodia. Pygidium smooth to slightly crenulate, with rounded ventral papilla ( Fig. 16 A–D, J, N). Tube unknown.

Remarks. Of the 14 species of Pista known to occur in Australian waters, seven have plumous branchiae: P. anthela Hutchings & Glasby, 1990 , P. australis Hutchings & Glasby, 1988 , P. curtiuncata Hartmann-Schröder, 1981 , P. gwoyarrma Hutchings, 1997b , P. kimberliensis Hutchings & Glasby, 1990 , and P. typha Grube, 1878 .

Of those, P. curtiuncata and P. typha clearly differ from P. anneae n. sp. in having segment 1 with large lobes, directed anteriorly and covering partially or completely the upper lip. Pista anthela , on the other hand, has a single pair of branchiae on segment 2 and short-handled uncini throughout, which are diagnostic features for the genus Pistella Hartmann-Schröder, 1996 and this species is herein transferred to that genus (see below).

Pista australis , described from southern Australia, differs from P. anneae n. sp. in lacking prostomial eyespots, and in having branchiae with different branching pattern, with large secondary stems; oblique, laterally higher lobes of segment 3; and broadly-winged, not geniculate notochaetae, with wings of similar size at both sides ( Hutchings & Glasby 1988). In P. anneae n. sp., on the other hand, eyespots are present in a thin continuous row across prostomium; branchial filaments are of uniform width, without enlarged secondary stems; lobes of segment 3 are rectangular, with straight lateral and distal margins; and notochaetae are geniculate, with rounded wings broader at one side.

Prostomial eyespots are also absent among specimens of P. gwoyarrma and, in addition, members of this species have lobes of anterior segments of different morphology in relation to P. anneae n. sp., those of segment 2 are larger, rectangular and those of segment 3 are oblique; and long-handled uncini are only present on segments 5 and 6 ( Hutchings 1997b). In P. anneae n. sp. the lobes of segment 2 are distally rounded and lobes of segment 3 are rectangular and larger, completely covering lobes of segment 2 in lateral view, while long-handled uncini are present until segment 10.

Pista kimberliensis also lacks prostomial eyespots and has lobes of anterior segments with different morphology, those of segment 2 are rectangular, visible all around, lobes of segment 3 distally rounded and inserted laterally, and long-handled uncini are only present until segment 9 ( Hutchings & Glasby 1990).

Etymology. This species is named “ anneae ” after Dr Anne Hoggett, a Co-Director of Lizard Island Research Station who strongly supported the workshop and helped in the development of the funding proposal to the LIRRF .

Type locality. Big Vicki´s Reef, 14°41'16"S, 145°26'35"E, Lizard Island, GBR, Australia.

Distribution. Known only from the Lizard Island region.

Figures

FIGURE 1. Live specimens. A – B. Nicolea murrayae n. sp. AM W. 44603, MI QLD 2397; C. Nicolea vaili n. sp. AM W. 44487, MI QLD 2335; D. Pista anneae n. sp. AM W. 44513, MI QLD 2370; E – H. Reteterebella lirrf n. sp. AM W. 44545, MI QLD 2416; I – J. Pista chloroplokamia n. sp., AM W. 44613, MI QLD 2400. Photos: A – J—Alexander Semenov.

FIGURE 16. Pista anneae n. sp. AM W. 44513: A – D. Entire worm, ventral, left dorso-lateral, left and right lateral views, respectively; E – H. Anterior end, left lateral, ventral, right lateral, and dorsal views, respectively; I, K – M. Close ups of the anterior end, ventral, right and left lateral, and dorsal views, respectively; J, N. Posterior end, right and left lateral views, respectively; O – Q. Progressively closer views of the anterior end, left lateral, ventral and right lateral views, respectively. Numbers refer to segments. Scale bars: A – D = 1 mm, E – H = 0.5 mm, I – N = 0.3 mm, O – Q = 0.2 mm.

FIGURE 18. Pista anneae n. sp. AM W. 44958: A. Close up of the anterior end, dorsal view; B – C. Closer views of the branchiae; D. Transition between anterior and posterior body, right lateral view; E. Notopodium of the segment 20; F – G, J – K. Progressively closer views of notochaetae, segment 10; H – I. Close ups of neuropodia, segments 5 – 6 and 10 – 11, respectively Scale bars: A, D = 200 µm, B = 100 µm, C, H = 40 µm, E – F = 30 µm, G, J = 10 µm, I = 50 µm, K = 15 µm.

FIGURE 19. Pista anneae n. sp. AM W. 44513: A – C. Notochaetae, segment 6; D – F. Notochaetae, segment 20; G – H. Uncini, segment 5; I, K. Uncini, segment 10; J, L. Uncini, segment 20. Scale bars: A = 30 µm, B – C, G = 15 µm, D = 40 µm, E – F, I, L = 20 µm, H, J – K = 10 µm.