Osmylus fulvicephalus ( Scopoli, 1763 )

Mao, Haohan, Liu, Zhiqi, Wu, San-An & Xu, Han, 2024, Re-description of 1 st instar larva of Osmylus fulvicephalus (Scopoli, 1763) (Neuroptera, Osmylidae) based on naming system of sclerites, Biodiversity Data Journal 12, pp. e 129392-e 129392 : e129392-

Osmylus fulvicephalus ( Scopoli, 1763)

Materials

Type status: Other material. Occurrence: recordedBy: Xu Han; individualCount: 2; sex: exs; lifeStage: first instar larva; occurrenceID: B616DC45-2212-5973-878D-7264A0E78B72; Taxon: scientificName: Osmylus fulvicephalus ; Location: country: Germany; stateProvince: Bayern; locality: surroundings of Frauenberg Hostel, Freyung, Bayern in Germany ; decimalLatitude: 48.7942; decimalLongitude: 13.7584; georeferenceProtocol: GPS; Record Level: collectionCode: Insects; basisOfRecord: PreservedSpecimen

Description

Colouration. Head capsule brown, except for black coloured ocular region; legs light yellow; dorsal side of thorax and abdomen basically light yellow to brown (Fig. 1 ).

Head capsule (Figs 2 , 3): Cranium bulb-like shaped, strongly sclerotised dorsally and laterally, surface smooth. Anterior edge of labrum with two pairs of short setae and two pores; frontal edge of ocular region with two long setae; posterior margin of acetabulum of antenna with two long setae and two pores; antenna (Fig. 3 a ) four-segmented, first segment trapezoidal, with two pores, second segment annulated, much longer than first one, third segment little longer than first one, with a pore and a long seta on median area, terminal segment short, apex narrow, bearing a long and two short setae; frons with two pairs of short setae and two pores along ecdysial line; four pairs of long setae and two pairs of pores on vertex; three pairs of short setae and a pair of pores on occiput. Mandible long, curved outwards and upwards, without distinct teeth, except for minute serrations at apex. Maxillary stylet long and smooth, without any serration and palpi, swollen at basal portion, bearing 11 pairs of pores. Intermediate maxillary elements ellipsoidal, attached to posterior margin of maxillary stylets, each laterally with two long setae. Proximal maxillary element similar to intermediate maxillary one in shape, without seta. Mentum large, antero-lateral angles protruding, with two long setae. Prementum inverted triangular, with two long setae. Labial palpi (Fig. 3 b ) four-segmented, first segment long distally with a long seta, a short seta and a pore; second one short without any seta and pore; third one short, slightly longer than second one, distally with two short setae; fourth segment long, gently tapered apically.

Thorax (Fig. 4 ) wider than head capsule, with numerous sclerites. Cervix (Fig. 4 ) narrower than cranium, very elastic, provided with a set of sclerites (paired L). L large, located in ventrolateral part, without any seta and pore.

Prothorax (Fig. 4 ): provided with three sets of sclerites (DPc; paired LPa + LPm + LPp, paired VP). DPc very large, located in the prothorax dorsally, with three pairs of short setae and two pairs of long setae anteriorly; a pair of long setae and a pair of middle setae medially; two pairs of middle setae and two pairs of long setae posteriorly. LPa + LPm + LPp large and long, three-branched, its anterior upside branch bearing three very short setae, middle branch attached with coxal cavity with a long seta and posterior branch bearing a long seta, located dorsally in coxal cavity. VP small, located in the interior part of coxal cavity, bearing a long seta.

Mesothorax (Fig. 4 ): provided with 16 sets of sclerites (paired DAa, paired DAm 1, paired DAm 2, paired DMa, paired DMp, paired DPa 1, paired DPa 2, paired DPm 2, paired DPp 1, paired DPp 2, paired LPa + LPm + LPp, paired VAa, VApc, paired VAm, paired VMa, paired VP). DAa large, located in the anterior margin of mesothorax dorsally, without any seta. DAm 1 and DAm 2 very small, located in the exterior and posterior part of DAa, each bearing a short seta. DMa large, located in the posterior part of DAm 1, with two short setae. DMp very small, located in the interior and posterior part of DMa, bearing a short seta. DPa 1 small, located in the interior and posterior part of DMp, bearing a long seta. DPa 2 large, located in the exterior and posterior part of DMa, bearing a long seta and two short setae. DPm 2 large, located in the posterior part of DMa, bearing three long setae. DPp 1 small, located in the posterior part of DPa 1, bearing a short seta. DPp 2 small, located in the posterior part of DPa 2, bearing a long seta. LPa + LPm + LPp large and long, three-branched, its anterior upside branch bearing three very short setae, middle branch attached with coxal cavity with a long seta and posterior branch bearing a long seta, located dorsally in coxal cavity. VAa small, located in lower part of spiracle, bearing a short seta. VAm small, located in the interior part of VAa, bearing a short seta. VApc small, located in the anterior margin of mesothorax centrally, without any seta. VMa very small, located in the exterior and posterior part of VApc, bearing a short seta. VP small, located in the interior part of coxal cavity, bearing a long seta.

Metathorax (Fig. 4 ) similar to mesothorax in conformation of sclerites, provided with 16 sets of sclerites (paired DAa, paired DAm 1, paired DAm 2, paired DMa, paired DMp, paired DPa 1, paired DPa 2, paired DPm 2, paired DPp 1, paired DPp 2, paired LPa + LPm + LPp, paired VAa, VApc, paired VAm, paired VMa, paired VP). DMa bearing only a short seta. DPp 1 large, bearing a long seta.

Legs (Fig. 5 ) well developed. Fore-leg (Fig. 5 ): coxa quadrate, bearing four long setae and four short setae on the exterior face, three long setae and five short setae on the interior face. Trochanter subtriangular, bearing a long seta, four short setae and a pore on the exterior face, a long seta, three short setae and two pores on the interior face. Femur long, exterior face with a long seta and a short seta on the proximal half and a long seta on the distal half, interior face with a short seta on the proximal half and a long seta and a short seta on the distal half. Tibia long, exterior face with a long seta on the proximal half and two long setae on the distal half, interior face with a pore on the proximal half and three long setae on the distal half. Tarsomeres fused forming one cylindrical segment, exterior face with a short seta, a long seta and many very short hairs at apex; two tarsal claws and one empodium long and triangular, bearing two short setae. Mid-leg (Fig. 5 ): coxa quadrate, bearing four long setae and four short setae on the exterior face, three long setae, seven short setae and a pore on the interior face. Trochanter subtriangular, bearing two long setae, a short seta and a pore on the exterior face, six short setae and a pore on the interior face. Femur long, exterior face with a long seta on the proximal half and two long setae on the distal half, interior face with a short seta on the proximal half and two short setae on the distal half. Tibia long, exterior face with three long setae on the distal half, interior face with a short seta on the proximal half and two long setae on the distal half. Tarsomeres fused forming one cylindrical segment, bearing many very short hairs at apex, exterior face with a short seta, a long seta and interior face with a short seta in the middle; two tarsal claws and one empodium long and triangular, bearing two short setae. Hind-leg (Fig. 5 ): coxa quadrate, bearing four long setae and four short setae on the exterior face, four long setae and five short setae on the interior face. Trochanter subtriangular, bearing a long seta, a short seta and a pore on the exterior face, a long seta, two short setae and a pore on the interior face. Femur long, exterior face with a long seta and a short seta on the proximal half and two long setae and a short seta on the distal half, interior face with a short seta on the proximal half and a long seta and two short setae on the distal half. Tibia long, exterior face with two long setae on the distal half, interior face with a short seta on the proximal half and three long setae on the distal half. Tarsomeres fused forming one cylindrical segment, bearing many very short hairs at apex, exterior face with a short seta and a long seta and interior face with a short seta in the middle; two tarsal claws and one empodium long and triangular, bearing two short setae.

Abdomen (Figs 6 , 7 ) 10 - segmented; spiracles present from first to eighth segments, each one partly sclerotised dorsolaterally. First abdominal segment: provided with 12 sets of sclerites (paired DA 1, paired DA 2, paired DM 2, paired DPp 1, paired DPp 2, paired DPp 4, paired LP, paired VA 1, paired VA 2, paired VM 1, paired VP 2, paired VP 4). DA 1 and DA 2 very small, located in the anterior part of segment dorsally, each bearing a short seta. DM 2 small, located in the posterior part of DA 1, bearing a short seta. DPp 1 large, located in the intero-posterior part of DPp 2, bearing a long seta. DPp 2 large, located in the posterior part of DM 2, bearing a long seta. DPp 4 large, size similar to DPp 1, located in the exterior part of DPp 2, bearing a long seta. LP large, located in the posterolateral part, bearing a long seta and a medium seta. VA 1 and VA 2 very small, located in the anterior part of segment ventrally, each bearing a short seta. VM 1 approximately oval, located in the posterior and interior part of VA 1, bearing a long seta. VP 2 and VP 4 large, located in the posterior part of VM 1, each bearing a long seta. Second to seventh abdominal segment: similar to first one in arrangements of sclerites, provided with 11 sets of sclerites (paired DA 1, paired DA 2, paired DM 2, paired DPp 1, paired DPp 2, paired DPp 4, paired LP, paired VA 2, paired VM 1, paired VP 2, paired VP 4). DPp 2 bearing a long seta, a medium seta and two pores. VP 2 and VP 4 each bearing a long seta and a medium seta. Eighth abdominal segment: similar to the seventh in conformation of sclerites, provided with eight sets of sclerites (paired DA 1, paired DA 2, paired DM 2, paired DPp 1 + DPp 2, paired LP, paired VA 2, paired VP 2, paired VP 4). DPp 1 + DPp 2 large, bearing two long setae, a medium seta and a pore. DPp 4 absent. LP bearing a long seta and two medium setae. VM 1 absent. Ninth abdominal segment: provided with three sets of sclerites (Dc, VPc; paired VA 2). Dc large, widely covering dorsalaterally, bearing two long setae and two short setae anteriorly; four short, six long setae and four pores posteriorly. VA 2 small, located in the antero-ventral part, bearing a short seta. VPc large, triangular, bearing six long setae. 10 th abdominal segment: provided with two sets of sclerites (Dc, VPc). Dc large, located in the segment ventral-posteriorly, bearing two short setae anteriorly; two long setae in the middle; two long setae and four short setae posteriorly. VPc large, widely covering ventrally, bearing five long setae, three short setae and three pores. Hook apparatus (Fig. 7 ) bearing nine lines of hooks on ventral surface of posterior half; hooks gradually becoming smaller basally.

Remarks

The morphology of the first instar larva of O. fulvicephalus differs greatly from the first instar larva of Stenosmylus tenuis (Walker, 1853) which was described by New (1974). The labial palpi of first instar larva of O. fulvicephalus is four-segmented, while three-segmented in latter species. The terminal segment of the antenna of O. fulvicephalus has a long and apical seta and two short setae while a long and apical seta and six short setae are present on that of latter species. Besides, the anal hooks of first instar larvae of O. fulvicephalus are arranged in nine transversal rows, while there is only a transversal row on anal apparatus of S. tenuis .

Based on the description of the third instar larva of Osmylus fulvicephalus recorded by previous studies ( Brauer 1851, Hagen 1852, Withycombe 1922, Rabaud 1927, Gepp 2003, Monserrat 2014) and our unpublished data, the morphological differences between the first and third instar larva of O. fulvicephalus are more than the numbers of setae and shape of empodium and the differences in the following morphological characteristics.

On the head capsule, two pairs of short setae are located along the ecdysial line of the first instar larva, while three pairs are present along that of the third instar larva. In addition, there are only two long setae present around the ocular region on each side, but three long setae present on the corresponding position of the third instar larva. The labial palpi of the 1 st instar larva is four-segmented, but five-segmented in the 3 rd instar larva.

On the thorax, the sclerite L on cervix of the first instar larva has no seta while a long seta presents this sclerite of the third instar larva. Three sets of sclerite DA 1, DA 2 and DM on the prothorax of the first instar larva are fused with DPc, but these sclerites are separated on that of the third instar larva. Moreover, the sclerites VAa and VAp on the prothorax of the first instar larva are absent while present on that of the third instar larva. The sclerite DPp 1 on mesothorax of the first instar is much smaller than DPa 1 while that of the third instar is approximately equal to Dpa 1 in size. VMp 1 and VMp 2 (mesothorax and metathorax) absent (existed). The sclerites LAa, LAm and LAp on the mesothorax and metathorax of 1 st instar larva are fused, but separated in the 3 rd instar larva. These three sets of sclerites are also separated in other osmylid larvae ( Matsuno and Yoshitomi 2016, Martins et al. 2018). On the legs, the empodium of the first instar larva is basally equipped with two slender setae, but that of the third instar is distally with numerous short setae as illustrated by Hagen (1852).

The dorsal side of abdomen is characterised by sclerite DM 2 on each of 1 st to 8 th abdominal segments of 1 st instar larva, much smaller than DPp 1, but same as DPp 1 of 3 rd instar larva in size. The sclerite DPa is absent on each of 1 st to 6 th abdominal segments of 1 st instar larva, but present on that of 3 rd instar larva. The sclerite DPp 3 is absent on each of 2 nd to 7 th abdominal segments of 1 st instar larva, but present on that of 3 rd instar larva. The sclerite DPp 4 is equipped with only one seta on each of 2 nd to 7 th abdominal segments of 1 st instar larva, but equipped with two setae on that of 3 rd instar larva. The sclerite LA is absent on each of 2 nd to 7 th abdominal segments of 1 st instar larva, but present on that of 3 rd instar larva. The sclerite LP is equipped with two setae on each of 1 st to 8 th abdominal segments of 1 st instar larva, but three on that of 3 rd instar larva. On the venter, the sclerite VA 3 is absent on each of 1 st to 8 th abdominal segments of 1 st instar larva, but present on that of 3 rd instar larva. The sclerite VM 2 on each of 2 nd to 5 th abdominal segments is absent in the first instar larva while present on that of the third instar larva. Additionally and likewise, the sclerite VM 3 on each of 2 nd to 6 th abdominal segments is also absent in the first instar larva, while present on that of the third instar larva. The sclerite VP 1 is absent on each of 1 st to 6 th abdominal segments of 1 st instar larva, but present on that of 3 rd instar larva. Similarly, the sclerite VP 3 is absent on each of 1 st to 7 th abdominal segments of the 1 st instar larva, but present on that of 3 rd instar larva. The sclerite VP 4 on each of 2 nd to 7 th abdominal segments of the 1 st instar larva is equipped with two setae, arranged in a longitude row, but only one seta on that of 3 rd instar larva.

Figures

Figure 1. The first instar larva of Osmylus fulvicephalus photographed in the laboratory rearing environment.

Figure 2. Head capsule of Osmylus fulvicephalus. a Dorsal view; b Ventral view.

Figure 4. Thorax of Osmylus fulvicephalus. a Dorsal view; b Ventral view.

Figure 5. Legs of Osmylus fulvicephalus. a Interior surface of legs; b Exterior surface of legs.

Figure 6. First to eighth abdominal segments of Osmylus fulvicephalus. a Dorsal view; b Ventral view.

Figure 7. 9 th to 10 th abdominal segments of Osmylus fulvicephalus. a Dorsal view; b Ventral view.

Figure 3 a. Antenna;

Figure 3 b. Labial palpi.