Ophiostoma massoniana Wang & Lu

Wang, HuiMin, Lun, YingYing, Lu, Quan, Liu, HuiXiang, Decock, Cony & Zhang, XingYao, 2018, Ophiostomatoid fungi associated with pines infected by Bursaphelenchusxylophilus and Monochamusalternatus in China, including three new species, MycoKeys 39, pp. 1-27 : 1

Ophiostoma massoniana Wang & Lu sp. nov. Figure 4

Etymology.

The epithet reflects the host tree, Pinus massoniana .

Type.

CHINA, Zhejiang Province, Fuyang City, from Monochamus alternatus gallery in Pinus massoniana infested by numerous PWN, November 2012, collected by Q Lu and YY Lun, culture ex-holotype, MUCL 55179 = CFCC 51648 = CXY1610.

Description.

Sexual form: Unknown. Asexual form: Hyalorhinocladiella -like. Conidiophores abundant, single, borne on aerial hyphae, (3.3 –)10.5–27.5(– 42.5) × (0.7 –)1.3–2.0(– 2.7) μm; conidia hyaline, single-celled, aseptate, obovoid or globose with pointed bases and rounded apices, (2 –)2.2–3.9(– 5) × (0.5 –)0.7–1.7(– 2) μm.

Culture characteristics.

Colonies on 2% MEA brown, the marginal hyphae sparse and radiating; some white mycelium produced early during growth that becomes black after 3-5 d. Colonies slowly growing, reaching 37.5 μm in diameter over 8 d at 25 °C, able to grow at 5 °C and 40 °C, with an optimal growth temperature of 30 °C; sporulation weak. On PDA culture medium, the colonies are dark brown; the mycelium is white, long and dense, with a daily growth of 4 μm at 25 °C.

Habitat and distribution.

Galleries of Monochamus alternatus in Pinus massoniana infested by PWN, in Zhejiang Province, China.

Additional specimens examined.

CHINA, Zhejiang Province, Yuyao City, from Monochamus alternatus galleries in Pinus massoniana infested by numerous PWN, November 2012, collected by Q Lu and YY Lun, MUCL 55180 = CFCC 51649 = CXY1611.

Note.

Ophiostoma massoniana , only known by its asexual, hyalorhinocladiella-like state, does not cluster in any of the 10 species complexes defined by de Beer and Wingfield (2013) in Ophiostoma s. l. According to the ITS and tub2 phylogenetic analysis, the species is related to O. saponiodorum and O. pallidulum (Figure 1). Ophiostoma pallidulum also only produces asexual hyalorhinocladiella-like morphs in vitro, whereas O. saponiodorum produces a sexual and two asexual morphs (pesotum-like and hyalorhinocladiella-like). In addition, O. massoniana differs from O. saponiodorum in producing smaller conidia [(2 –)2.2–3.9(– 5) × (0.5 –)0.7–1.7(– 2) μm vs. (3 –)4–6(– 7) × 1 –1.5(– 2) μm] ( Linnakoski et al. 2010). Further, the colour of O. massoniana colonies is different from that of the other two species. Namely, O. massoniana forms brown to dark brown colonies, while the other two species form pale colonies ( Linnakoski et al. 2010).