Neomonodictys aquatica D.F. Bao, S.P. Huang & Z.L. Luo, 2022

Huang, Si-Ping, Bao, Dan-Feng, Shen, Hong-Wei, Su, Hong-Yan & Luo, Zong-Long, 2022, Neomonodictys aquatica sp. nov. (Pleurotheciaceae) from a plateau lake in Yunnan Province, China, Biodiversity Data Journal 10, pp. 76842-76842 : 76842

Neomonodictys aquatica D.F. Bao, S.P. Huang & Z.L. Luo sp. nov.

Materials

Type status: Holotype. Occurrence: recordedBy: Longli Li; Siping Huang ; Taxon : scientificName: Neomonodictys aquatica; kingdom: Fungi ; phylum: Ascomycota ; class: Sordariomycetes ; order: Pleurotheciales ; family: Pleurotheciaceae ; genus: Neomonodictys ; Location : waterBody: Erhai Lake ; locality: Baitaiyi ; verbatimElevation: 1966 m; locationRemarks: China, Yunnan Province, Dali , saprobic on submerged decaying wood in Erhai Lake ; verbatimLatitude: 25 44 29.65N; verbatimLongitude: 100d 09' 49.33'' E; Event : year: 2020; habitat: freshwater, submerged decaying wood; Record Level : collectionID: 2EH 3-17-1 H; collectionCode: L127.

Description

Sexual morph Undetermined. Asexual morph Hyphomycetous (Fig. 1 ) sporodochia. Colonies on natural substratum superficial, scattered, black, glistening. Mycelium immersed in the substrate, composed of septate, smooth, thin-walled, light to dark brown, 2-3 μm wide hyphae. Conidiophores lacking. Conidiogenous cells short or occasionally missing, suborbicular, holoblastic, monoblastic, integrated, terminal, determinate, hyaline to pale brown. 3.7-6.4 × 2.9-4.7 μm (x̄ = 5.1 × 3.8 μm, n = 10). Conidia 23.1-29.5 × 8.5-11.5 μm (x̄ = 26 × 10 μm, n = 30), acrogenous, acrospore, oval, ellipsoidal to obovoid, muriform, smooth-walled, hyaline when young, becoming dark brown at maturity sometimes with one pale basal cell.

Culture characteristics: Conidia germinate on PDA in 36 h. Colonies growing on PDA, subglobose, with flat surface, edge jagged, reaching 3 cm long and 2.5 cm wide in 12 weeks at 28°C, dark grey in PDA medium. Mycelium superficial and partially immersed, branched, septate, hyaline to pale brown, smooth.

Material examined: China, Yunnan Province, Dali, sprobic on submerged decaying wood in Erhai Lake, September 2020, S. P. Huang, L-127 (KUN-HKAS 115806, holotype), ex-type living culture, KUNCC 21-10708 = CGMCC3.20681.

Etymology

Name reflects the aquatic habitat of this fungus

Notes

Morphologically, Neomonodictys aquatica is easily distinguished from N. muriformis . Neomonodictys muriformis has wider conidia than N. aquatica (15-25 vs. 8-12.2 μm). In addition, conidia of N. aquatica are oval or ellipsoidal to obovoid, while N. muriformis has subglobose to globose conidia. In the phylogenetic analysis, N. aquatica clustered with N. muriformis with strong support (99% ML and 1.00 PP) (Fig. 2 ). ITS comparison between our strain and MFLUCC 16-1136 revealed 57 bp difference in a total of 539 bp. LSU comparison between our strain and MFLUCC 16-1136 revealed 13 bp difference in total of 829 bp ( Jeewon and Hyde 2016). Therefore, we introduce our new isolate as a new species.

Figures

Figure 1. Neomonodictys aquatica (KUN-HKAS 115806, holotype). a Colonies on submerged wood; b-e Conidiophores with conidia; f-j Conidiogenous cells with conidia; k-p Conidia; q Germinating conidium; r, s Colony on PDA. Scale bars: b-c, e, k = 25 μm; d, f-i, l-p = 20 μm; j, q = 30 μm.

Figure 2. Phylogenetic tree based on RAxML, generated from a combined ITS and LSU dataset. Bootstrap support values for Maximum Likelihood (ML, black) higher than 75 % and Bayesian posterior probabilities (BYPP, red) greater than 0.95 are indicated above the nodes as ML / PP. The tree is rooted to Leotia lubrica. The type-derived sequences are indicated in bold and new isolates are in red. Bootstrap values for Maximum Likelihood (ML) equal to or greater than 75 % and clade credibility values greater than 0.90 from Bayesian-inference analysis labelled on the nodes. Ex-type strains are in bold and black, the new isolate is indicated in bold and red. (Fig. 2).