Neomicrosphaeropsis cylindrica S. M. Fu & Yong Wang bis, 2024

Fu, Shamin, Sun, Jing-E, Tarafder, Entaj, Wijayawardene, Nalin N., Hu, Yan, Wang, Yong & Li, Yan, 2024, Pezizomycotina species associated with rotten plant materials in Guizhou Province, China, MycoKeys 106, pp. 265-285 : 265-285

Neomicrosphaeropsis cylindrica S. M. Fu & Yong Wang bis sp. nov.

Fig. 6

Etymology.

In reference to the fungus, which produced cylindrical conidia.

Diagnosis.

Neomicrosphaeropsis cylindrica is characterised by broadly cylindrical conidia (15.4 × 3.4; L / W = 4.574) with moderate growth rate.

Type.

China, Guizhou Province, Guiyang City , 26 ° 57 ' N, 106 ° 72 ' E, from rotten dead leaves, 19 July 2023, S. M. Fu, HGUP 23-0015 (holotype), ex-type culture GUCC 23-0048 .

Culture characteristics.

Saprobic on dead leaves. Colony on PDA, 35–38 mm diameter, after 7 days, dense low-altitude hypha, light yellow, centre with abundant stigma; Turning light yellow to rose light yellow, the centre of concentric circles is darker; on MEA, after 7 days, 28–30 mm, the edge is intact, dense hypoxic mycelium, the edge is yellowish; reverse rose-yellowish to yellowish at margin with abundant scattered on stigma; Conidia cylindrical, spherical to kettle-shaped, 200–350 μm in diameter, tan to black, solitary, population centre abundant, banded, glabrous, without papillae; the cell wall is angular textured, light brown, bifid, cylindrical, thin-walled, transparent. Conidia occasionally septate, 12.5–18.5 × 2.4–4.0 μm (x ̄ = 15.4 × 3.4 μm, n = 30), cylindrical, transparent, thin-walled.

Sexual stage.

Not observed.

Habit.

On rotten dead leaves.

Distribution.

China, Guizhou Province, Guiyang City.

Other materials examined.

China, Guizhou Province, Guiyang City , 26 ° 57 ' N, 106 ° 72 ' E, from rotten dead leaf, 19 July 2023, S. M. Fu, HGUP 23-0015 , living culture GUCC 23-0048 , GUCC 23-0049 and GUCC 23-0050 .

Notes.

Neomicrosphaeropsis cylindrica ( GUCC 23-0048 ) formed a clade with N. rossica ( MFLUCC 14-0586 ) and N. alhagi-pseudalhagi ( MFLUCC 17-0825 ) (Fig. 3 ). However, by morphological comparison, our species produced obviously longer conidia than N. rossica (12.5–18.5 × 2.4–4.0 μm vs. 4.4–5.7 × 2.9–3.9 μm) and smaller conidia than N. alhagi-pseudalhagi (12.5–18.5 × 2.4–4.0 μm vs. 30–45 × 18–22 μm) ( Thambugala et al. 2017; Wanasinghe 2018).

Figures

Figure 3. Trees resulting from ML analysis of the combined ITS, SSU and LSU sequence alignment for twelve isolates in Neomicrosphaeropsis and eight isolates in Microsphaeropsis. RAxML and MP bootstrap support values (ML, MP ≥ 60 %) and Bayesian posterior probability (PP ≥ 0.65) are denoted on the nodes (ML / MP / PP). The tree was rooted to Neodidymelliopsis ranunculi (MFLUCC 13-0490). New species are highlighted in red. The scale bar indicates 0.002 expected changes per site. T = ex-holotype strain.

Figure 6. Neomicrosphaeropsis cylindrica (GUCC 23-0048) a appearance on host surface b – e culture characteristics on PDA, WA (b – d above view c – e reverse view) f – g colonies on PDA h – l conidiophores, Conidiogenous cells and Conidia m conidia. Scale bars: 100 µm (h); 50 µm (i – k); 20 µm (l – m).