Neohormodochis septispora D. P. Wei & K. D. Hyde, 2022

Wei, De-Ping, Gentekaki, Eleni, Wanasinghe, Dhanushka N., Hyde, Kevin D., To-Anun, Chaiwat & Cheewangkoon, Ratchadawan, 2022, Neohormodochis septispora gen. et sp. nov. (Stictidaceae) from Yunnan Province, China, Phytotaxa 573 (2), pp. 247-261 : 254-255

Neohormodochis septispora D. P. Wei & K. D. Hyde , sp. nov. ( Figure 2 )

Index Fungorum number: IF 559772

Faces of Fungi number: FoF12298

Etymology: The epithet “ septispora ” refers to the septate conidia.

Holotype: China, Yunnan Province, Kunming, Panlong district, Kunming Institute of Botany , on an unidentified dead twig, 31 March 2021, Cuijinyi Li, KLCJY54 ( HKAS 124171 , holotype), ( KUNCC 22-10806 , ex-type culture).

Saprobic on an unidentified dead twigs. Sexual morph: Undetermined. Asexual morph: Conidiomata 165–360 × 140–340 (x̄ = 282 × 252, n = 10) μm, perithecial, immersed, conical to subglobose, white-pruinose, gregarious, with olivaceous green content. Conidiomatal wall 11–30 (x̄ = 19, n = 30) μm, consisting of hyaline, intricate hyphae, being encompassed by a layer of irregularly-shaped crystals. Conidiophores reduced to conidiogenous cells. Conidiogenous cell 3.5–10 × 0.8–2.4 (x̄ = 7 × 1.6, n = 25) μm, arising from stromatic cells, hyaline, cylindrical, thin-walled, the first conidium becoming conidiogenous by apical wall-building to form connected chain. Conidia 7.6–12 × 2–3.8 (x̄ = 9.5 × 3.3, n = 40) μm, hyaline, ellipsoidal, two-celled, occasionally asepate, slightly curved, arranged in branched chain.

Culture characteristics: isolates were obtained from germinating conidia. Colony slow-growing, reaching 2 cm after 8 weeks on PDA, white, circular, mycelia dense, velvety, reverse creamy-yellow.

Additional materials examined: China, Yunnan Province, Kunming, Panlong district , Heilongtan Park, 31 March 2021, Cuijinyi Li , HLCJY57 ( HKAS 124172 ), ( KUNCC22-10807 , living culture) ; HLCJY58 ( HKAS 124174 ), ( KUNCC22-10808 , living culture) ; HLCJY59 ( HKAS 124173 ), ( KUNCC22-10809 , living culture); ibid. Songhuaba reservoir, 11 December 2021, De-Ping Wei , SHB1225 ( HKAS 124170 ), ( KUNCC22-10810 , living culture) .

Notes: Phylogenetically, Neohormodochis septispora has a sister affiliation to a clade comprising Hormodochis aggregata , H. melanochlora and H. eucalypti ( Figure 1 ). Morphologically, N. septispora is similar with H. aggregata by catenulate conidia but differs by the hyaline, ellipsodial and septate conidia while it is olivaceous brown, subcylindrical to doliiform, and aseptate in the latter ( Crous et al. 2020a). Hormodochis melanochlora is distinct by erumpent, globose, brown conidiomata that are immersed, conical to globose, white-pruinose from N. septispora ( Crous et al. 2020a) . Hormodochis eucalypti (syn. Phacidiella eucalypt ) has dark brown to black conidiomata and subcylindrical to barrel-shaped conidia that are dramatically different from N. septispora ( Crous et al. 2007, 2020b).

Figures

FIGURE 1. Phylogram generated from a maximum likelihood analysis based on concatenated ITS, LSU, mtSSU and RPB2 sequence dataset. Bootstrap support (MLBS) equal to or greater than 60% and Bayesian posterior probabilities (PP) equal to or higher than 0.95 are given above the nodes. Generic names are noted on the right side. Newly generated sequence are highlighted in blue bold font.

FIGURE 2. Neohormodochis septispora (HKAS 124171, holotype). a Substrate. b, c Conidiomata. d, e Vertical section through conidiomata. f Crystals. g Conidiomatal wall. h, i Conidiogenous cells bearing conidia. j, k Conidial chains. l, m Developing conidia. n, o Conidia. p Germinal conidium. q, r Upper and lower view of cultures. Scar bar: d, e = 150 um, f, g, j, p = 30 um, h, i, k, n, o = 10 um, l, m = 5 um.