Kockovaella ischaemi Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 81-83

Kockovaella ischaemi Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828738. Fig. 7C, D .

Etymology: the specific epithet ischaemi refers to Ischaemum , the plant genus from which the type strain was isolated.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal or ovoid, 2.0–3.8 × 2.3– 6.2 μm and single or pairs, budding is polar ( Fig. 7C ), blastoconidia are produced on short stalk-like conidiophores, a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is yellowish-cream, butyrous, smooth and glistening. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are ellipsoidal or some what kidney-shaped, 2.0–3.7 × 4.2– 6.7 μm ( Fig. 7D ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, inulin, soluble starch, D-xylose, L-arabinose, D-arabinose (weak), Dribose, L-rhamnose, D-glucosamine, N-Acetyl-D-glucosamine, glycerol (variable), ribitol (variable), galactitol, D-mannitol, Methyl-α- D-glucoside (variable), salicin (weak), succinate (weak), citrate (variable) and myo-Inositol (variable) are assimilated as sole carbon sources. L-sorbose, methanol, ethanol, erythritol, D-glucitol, DL-lactate and hexadecane are not assimilated. Ammonium sulfate, sodium nitrite and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Potassium nitrate, L-lysine and ethylamine hydrochloride are not assimilated. Maximum growth temperature is 30 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive. The major ubiquinone is Q-10.

Physiologically, Koc. ischaemi differs from the closely related species Koc. haikouensis in its inability to assimilate ethylamine and its ability to assimilate inulin, D-arabinose, L-rhamnose and sodium nitrite ( Table S1.1 ).

Typus: China, Jinghong, Yunnan province, obtained from a leaf of Ischaemum sp. , Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3565 T preserved in a metabolically inactive state, ex-type CBS 15500 = JH5.17)

Figures

Fig. 7. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Koc. haikouensis CGMCC 2.3443T; (C, D) Koc. ischaemi CGMCC 2.3565T; (E) Koc. nitrophila CGMCC 2.3465T; (F) G. pseudoamylolytica CGMCC 2.5809T; (G) Tr. shuangheensis CGMCC 2.5615T; (H) V. melezitolyticum CGMCC 2.3472T; (I) V. pseudopenaeus CGMCC 2.3165T; (J) V. europaea CGMCC 2.3099T; (K) Ca. follicola CGMCC 2.3447T; (L) Ca. simaoensis CGMCC 2.3580T; (M) Kwoni. ovata CGMCC 2.3439T; (N) Te. helanensis CGMCC 2.4450T; (O) Te. globosa CGMCC 2.5648T; (P) Te. korlaensis CGMCC 2.3835T. Bars = 10 μm.