Klebsormidium mirabile Tukhtaboeva, Krivina, Redkina & Temraleeva, 2025

Tukhtaboeva, Yulduzkhon, Krivina, Elena, Redkina, Vera, Portnov, Aleksey, Sinetova, Maria & Temraleeva, Anna, 2025, Species delimitation in the genus Klebsormidium (Klebsormidiophyceae, Charophyta), including description of Klebsormidium mirabile sp. nov. with high content of polyunsaturated fatty acids, PhytoKeys 266, pp. 53-74 : 53-74

Klebsormidium mirabile Tukhtaboeva, Krivina, Redkina & Temraleeva sp. nov.

Description.

Filaments predominantly uniseriate and unbranched, curved, with a medium or long length (over 100 cells), easily disintegrated into short fragments (sometimes unicellular) and forming flake-like colonies in liquid culture and felt-like colonies with clusters of air filaments on agar plate. In young cultures cells mostly cylindrical or barrel-shaped with moderately thickened cell walls (sometimes with H-pieces) and slightly visible or prominent constrictions near the cross walls. Cell sizes (5.7) 7.1–18.6 (24) × 7.2–10.5 µm with a length to width ratio of 0.6–3. In old cultures cells elongated, barrel- or bean-like, often curved or asymmetrical. Chloroplast single and parietal, girdle-shaped with a wavy or smooth margin, which occupied more than one half of cell periphery. Pyrenoid single, spherical, small and compact, surrounded by several distinct starch grains. Nucleus clearly visible and situated opposite to the pyrenoid. Mucilage present. Vegetative multiplication by fragmentation of filaments into unicellular or few-cellular fragments. Zoospores, aplanospores, gametes, akinetes not observed.

Holotype.

Cryopreserved material in a metabolically inactive state from authentic culture VKM Al-436 stored under designation VKM Al-436 cryo in the All-Russian Collection of Microorganisms ( VKM) at the Skryabin Institute of Biochemistry and Physiology of Microorganisms at the Pushchino Biological Research Center of the Russian Academy of Sciences, Pushchino, Russia .

Authentic culture.

Culture of authentic strain VKM Al-436 was kept at the All-Russian Collection of Microorganisms ( VKM) at the Skryabin Institute of Biochemistry and Physiology of Microorganisms at the Pushchino Biological Research Center of the Russian Academy of Sciences, Pushchino, Russia.

Representative illustration.

Fig. 1 A – H .

Molecular analyses.

Phylotype ( OR 838744 ) differs from other species of genus Klebsormidium in rbc L phylogeny.

Habitat.

Soil.

Type locality.

Uzbekistan, the Chust district of the Namangan region, from soil of the Chust-Pop Hills in the Fergana Valley , 40°52'37.14"N, 70°59'44.76"E .

Etymology.

The specific epithet is coined for the wonderful appearance of our new species.

Figures

Figure 1. Morphology of strain Klebsormidium sp. VKM Al- 436. A. General view of filaments and solitary cells; B. Cell with “ papilla ” (marked with arrow); C. H-pieces of cell wall; D. Cells with clearly visible nucleus and nucleolus; E. Formation of biseriate filaments; F. Flake-like colonies in liquid culture; G. Felt-like colonies on agar plate; H. Clusters of air filaments on agar plate; I. Clusters of filaments stained with India ink. Scale bars: 10 μm (A – E), 50 μm (H – I).