Hemicycliophora undetermined

Subbotin, Sergei A., Chitambar, John J., Chizhov, Vlamidir N., Stanley, Jason D., Inserra, Renato N., Doucet, Marcelo E., Clure, Michael M, Ye, Weimin, Yeates, George W., Mollov, Dimitre S., Cantalapiedra-Navarrete, Carolina, Vovlas, Nicola, Berg, Esther Van Den & Castillo, Pablo, 2014, Molecular phylogeny, diagnostics, and diversity of plant-parasitic nematodes of the genus Hemicycliophora (Nematoda: Hemicycliophoridae), Zoological Journal of the Linnean Society 171 (3), pp. 475-506 : 493-494

HEMICYCLIOPHORA SPECIES

The D2-D3 of the 28S rRNA gene alignment included 102 sequences of Hemicycliophora and two Paratylenchus sequences selected as outgroup taxa and was 685 bp in length. Ninety-two new D2-D3 of 28S rRNA gene sequences were obtained in the present study. Intraspecific sequence diversity (uncorrected p-distance) for the populations of some species were: H. thienemanni – 0–0.8% (0–5 bp), H. gracilis – 0–0.5% (0–3 bp), H. iberica – 0.2–1.0% (2–7 bp), Hemicycliophora sp. 4 – 0–0.3% (0–2 bp), Hemicycliophora sp.9 – 0–0.4% (0–2 bp), H. poranga – 0–0.7% (0–7 bp), H. wyei – 0–0.7% (0–5 bp), H. conida – 0–0.6% (0–4 bp). The minimal interspecific differences observed were for Hemicycliophora sp. 9 vs. Hemicycliophora sp. 13 – 0.3– 0.4% (2–3 bp), vs. H. epicharoides – 0.3% (2 bp), and vs. H. typica – 0.4–0.6% (3–5 bp).

The ITS of the rRNA gene alignment included 97 sequences of Hemicycliophora and two sequences selected as outgroups from the genera Paratylenchus and Gracilacus and was 874 bp in length. Eighty-eight new ITS of rRNA gene sequences were obtained in the present study. Intraspecific sequence diversity for populations of H. thienemanni – 0.1–1.2% (1–9 bp), H. gracilis – 0.1–0.8% (1–6 bp), H. iberica – 0.4–1.7% (3–13 bp), Hemicycliophora sp. 4 – 0–1.5% (0–10 bp), H. epicharoides – 0–1.5% (0–11 bp), H. poranga – 0–1.3% (0–9 bp), H. wyei – 0–0.9% (0–7 bp). Minimal interspecific differences were for Hemicycliophora sp. 13 vs. H. epicharoides – 2.0–2.6% (15–19 bp) and vs. H. typica – 2.7–3.6% (20–26 bp).