Duplominona bocasana Curini-Galletti, 2019

Curini-Galletti, Marco, Stocchino, Giacinta A. & Norenburg, Jon L., 2019, New species of Duplominona Karling, 1966 and Pseudominona Karling, 1978 (Platyhelminthes: Proseriata) from the Caribbean, Zootaxa 4657 (1), pp. 127-147 : 134

Duplominona bocasana Curini-Galletti n. sp.

( Fig. 3 F–J)

Holotype. Panama: Caribbean Sea : Bocas del Toro Is. (Lat. 9.364545, Lon. -82.238835), lower intertidal in coarse sand, sheltered by coral reef, June 2010: one whole mount ( USNM 1569256 ).

Other material. Same data as holotype, three specimens studied karyologically.

Etymology. The species is named after the type locality, Bocas del Toro.

Description. A small species, similar to the previous species in general morphology ( Fig. 3 F ).

Male genital system. With 8–10 testes in one irregular row. Cirrus about 40–70 μm long, provided with 8–15 rows of spines, with relatively few spines per row ( Figs 3 G–J). Proximal spines larger, 5-6 μm long, straight, with narrow bases, 2–2.5 μm wide, with a somewhat obtuse distal tip. More distally, spines become more curved in shape, with proportionally wider bases. Distalmost spines become progressively smaller, to 2.5–3 μm high, and 2.5–3 μm wide. Near the top, spines are small, 1.5–2.5 μm high, 1.5–2.5 μm wide, only slightly curved.

Prostatoid organ just posterior to copulatory bulb, opening to the outside through it own pore. Prostatoid stylet about 20 μm long.

Female genital system. Ovaria and vitellaria as in previous species. With a distinct bursa just in front of copulatory bulb, with external vagina ( Fig. 3 F ). Female pore posterior to prostatoid organ pore.

Karyotype. With n=3, and all chromosomes acrocentric, different in size. Karyotype formula: FN=3; Chromosome I: 57.8; 10.8 (a); Chromosome II: 25.14; 10 (a); Chromosome III: 17.08; 17.24 (st) (based on one plate).

Diagnosi s. Small Duplominona species with up to 10 testes in one row. Cirrus long and narrow, with 8–15 rows of narrowly triangular spines up to 6 μm long proximally, becoming progressively more curved and with wider bases medially. Distal spines progressively smaller, to 1.5 μm long. With a prostatoid organ provided with a stylet 20 μm long. With a bursa and an external vagina opening close to male pore. Female duct and prostatoid organ open to the outside independently. Karyotype with three pairs of acrocentric chromosomes

Figures

FIGURE 3. (A–E) Duplominona pusilla n. sp. (A) Habitus of a living animal; see Fig. 1 for interpretation of anatomical features. (B) Reconstruction of the inverted cirrus showing the sclerotised copulatory structures. (C, D) Micrographs of sagittal sections of the genital area. (E) Reconstruction of the genital organs from sagittal sections. (F–J) Duplominona bocasana n. sp. (F) Habitus of a living animal; see Fig. 1 for interpretation of anatomical features. (G, H) Reconstructions of cirri (G: inverted; H: everted) showing the sclerotised copulatory structures. (I, J) Micrographs of inverted (I) and everted (J) cirri from a karyological slide (Scale bars = 10 m).