Celoporthe hawaiiensis Kamgan, Jol. Roux & Marinc., 2020

Roux, Jolanda, Kamgan Nkuekam, Gilbert, Marincowitz, Seonju, van der Merwe, Nicolaas A., Uchida, Janice, Wingfield, Michael J. & Chen, ShuaiFei, 2020, Cryphonectriaceae associated with rust-infected Syzygium jambos in Hawaii, MycoKeys 76, pp. 49-79 : 49

Celoporthe hawaiiensis Kamgan, Jol. Roux & Marinc. sp. nov. Fig. 4

Etymology.

The species name refers to the Hawaiian Islands where the holotype was collected.

Types.

Holotype: USA, Hawaii, Maui Island, Hana Road, 20 miles from Kahului, isolated from bark of Syzygium jambos , 30 July 2012, J. Roux (PREM61307; Ex-type culture CMW38610 = CBS140642); GenBank accession numbers KJ027499 (ITS), KJ027475 (BT1), KJ027484 (TEF1). Paratypes: Hawaii, Maui Island, Hana Road, 20 miles from Kahului, isolated from bark of Syzygium jambos , 30 July 2012, J. Roux (PREM 61308; living culture CMW38582 = CBS140643). Hawaii, Big Island, Rainbow Falls, Hilo, isolated from bark of Syzygium jambos , 26 July 2012, J. Roux (CMW38553).

Sexual morph.

Not observed.

Asexual morph.

Formed after two months on Eucalyptus stem sections placed on water agar. Conidiomata superficial or with base embedded, single or gregarious, uni- or multilocular, convoluted, base often covered with brown hyphal mass, dark brown to black, pulvinate to conical with or without necks, 450-1814 µm long, 329-1069 µm wide; necks attenuating towards apex, tip of neck paler than body. Conidiomatal wall outer-and inter-locular stratum prosenchymatous; inner fertile stratum pseudoparenchymatous, 5-19 µm thick. Paraphyses present, cylindrical, tapering towards apex, scarce, 16-29 µm long. Conidiophores formed along inner layer of locule, simple or branched, occasionally reduced to conidiogenous cell, 10-26 µm long. Conidiogenous cells enteroblastic, lageniform, tapering towards apex, 4-12 × 1-2 µm. Conidia hyaline, oblong, aseptate, exuding in yellow droplets or tendril, 2.5-4 × 1-1.5 (3.17 ± 0.27 × 1.27 ± 0.08) µm.

Culture characteristics.

Colonies on 2% MEA, when young showing circular growth with smooth margins, above white with yellow tint towards edge (25 °C), reverse pale brown, becoming darker in centre at 25 °C and 30 °C; with age above becoming darker yellow to brown, reverse dark brown, except at 20 °C, 25 °C having yellow with dark brown patches; optimal growth at 30 °C (6.6 mm/d), followed by 25 °C (6.0 mm/d) and 20 °C (4.1 mm/d), minimal growth at 35 °C (0.1 mm/d), growth at 5 °C restricted to mycelial plug; mycelia fluffy, density sparse in centre becoming thicker towards the edge.

Habitat.

On/in bark of Psidium cattleianum , Syzygium jambos and Syzygium sp. indet.

Distribution.

Hawaii, USA

Notes.

Celoporthe hawaiiensis is morphologically similar to Cel. guangdongensis and Cel. paradisiaca , its phylogenetic closest relatives, but can be differentiated by DNA sequences. In the ITS, BT1 and TEF1 datasets, Cel. hawaiiensis differs from Cel. guangdongensis by 3, 3 and 1 base pairs and from Cel. paradisiaca by 6, 3 and 3 base pairs, respectively (Tables 6 , 7 ).

Figures

Figure 4. Micrographs of Celoporthe hawaiiensis sp. nov. (holotype: PREM 61307, ex-holotype CBS 140642 = CMW 38610) A culture morphology on 2 % MEA at 25 ° C and 30 ° C at 9 and 27 days B, C conidiomata produced on Eucalyptus stem sections on water agar D, E vertical section of conidioma F conidiomatal wall G, H conidiogenous cells I conidia. Scale bars: 1 mm (B, C); 100 µm (D, E); 10 µm (F, G); 5 µm (H, I).