Carlosrosaea simaoensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 85-86

Carlosrosaea simaoensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828746. Fig. 7L .

Etymology: the specific epithet simaoensis refers to the geographic origin of the type strain, Simao county, Yunnan.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal, 2.0– 2.6 × 3.3– 4.2 μm and single, budding is polar ( Fig. 7L ), a sediment is present. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is white-cream, butyrous, smooth and glossy. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, cellobiose, trehalose, lactose, melibiose, raffinose, melezitose, soluble starch, D-xylose, L-arabinose, D-arabinose (delayed and weak), D-ribose, L-rhamnose (delayed and weak), D-glucosamine, ethanol, glycerol, ribitol, galactitol (delayed and weak), Dmannitol, D-glucitol, Methyl-α- D-glucoside, salicin, DL-lactate (delayed and weak), succinate (weak), citrate and myo-inositol (delayed and weak) are assimilated as sole carbon sources. Lsorbose, inulin, methanol, erythritol and hexadecane are not assimilated. Ammonium sulfate is assimilated as sole nitrogen sources. Potassium nitrate, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 28 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ca. foliicola and Ca. simaoensis , and their three closely related species, Ca. vrieseae , Ca. hohenbergiae and Ca. aechmeae , can be distinguished from each other by the ability to assimilate inulin, erythritol, L-lysine and cadaverine and form starch like compounds ( Table S1.5 ).

Typus: China, Simao county, Yunnan province, obtained from a leaf of an unidentified plant, Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3580 T preserved in a metabolically inactive state, ex-type CBS 15503 = SM8.1).

Figures

Fig. 7. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Koc. haikouensis CGMCC 2.3443T; (C, D) Koc. ischaemi CGMCC 2.3565T; (E) Koc. nitrophila CGMCC 2.3465T; (F) G. pseudoamylolytica CGMCC 2.5809T; (G) Tr. shuangheensis CGMCC 2.5615T; (H) V. melezitolyticum CGMCC 2.3472T; (I) V. pseudopenaeus CGMCC 2.3165T; (J) V. europaea CGMCC 2.3099T; (K) Ca. follicola CGMCC 2.3447T; (L) Ca. simaoensis CGMCC 2.3580T; (M) Kwoni. ovata CGMCC 2.3439T; (N) Te. helanensis CGMCC 2.4450T; (O) Te. globosa CGMCC 2.5648T; (P) Te. korlaensis CGMCC 2.3835T. Bars = 10 μm.