Campiglossa melaena (Hering)

Han, Ho-Yeon & Ro, Kyung-Eui, 2019, DNA barcoding reveals a species group of the genus Campiglossa (Diptera, Tephritidae, Tephritinae) with recognition of a new species from East Asia and previously unknown females of Campiglossa coei (Hardy), ZooKeys 899, pp. 1-36 : 1

Campiglossa melaena (Hering) Figs 5 G–K , 9 A–C

Sinotephritis melaena Hering, 1941: 27 (Type-locality: China: Manchuria, Sjaolin. Holotype ♂, allotype ♀, NHMUK).

Campiglossa melaena : Korneyev 1990: 443 (new combination); Wang 1998: 255, 265 (in the East Asian Campiglossa key, diagnosis); Norrbom et al., 1999: 112 (in world Tephritidae catalog); Korneyev and Ovchinnikova 2004: 545 (in the Russian Far East Tephritidae key).

Material examined

. Russia: Primorsky-Krai : Khasansky-District , Barabash , 43°10'46.9"N, 131°28'20.0"E, 61 m, 22.VI.2008, H.Y. Han and H.S. Lee, 3♂ (YSUW); Nadezhdinsky-District , Vol’no-Nadezhdinskoye, grassland near restaurant, 43°22'31.6"N, 132°01'43.1"E, 61m, 22.VI.2008, H.Y. Han and H.S. Lee, 3♂ ( YSUW).

Diagnosis.

This is the darkest species of the misella group, showing the least wing dimorphism ( Fig. 7G, I, J vs. K). Head largely brown with dark grey upper occiput. Thorax with dark grey scutum with five brownish longitudinal stripes ( Fig. 7H ); scutellum dark grey; Legs with coxae and femora largely dark grey but tibiae and tarsi brown; fore femur with 5-7 dark brown posteroventral setae. Wing almost entirely brown to dark brown with numerous hyaline spots; male with large dark mid-anterior marking covering from pterostigma to posterior end of crossvein R-M; male pterostigma almost completely dark brown, at most with tiny hyaline spot ( Fig. 7I-a ); female pterostigma with larger hyaline spot ( Fig. 7K-a ); cell r1 posterior to pterostigma with three large hyaline spots in both sexes; cell r2+3 with posteroapical hyaline spot ( Fig. 7I-b ). Abdomen almost entirely dark grey.

Distribution.

North east China, the Russian Far East.

Remarks.

Hering’s (1941) original description and wing drawing of the holotype from north east China fall clearly within the variation range of the specimens we obtained from the Russian Far East. Unfortunately, we were not able to collect any female specimens, but Korneyev and Ovchinnikova’s (2004) illustrations ( Fig. 7J, K ) show a similar sexual dimorphism of the wing pattern as in the other misella group species. Individuals of C. melaena have DNA barcodes ( Figs 1 , 2 ) indistinguishable from those of C. paramelaena sp. nov. (see the Remarks of the latter species for further discussion).

Figures

Figure 1. The genus Campiglossa portion of the neighbor-joining tree based on the Kimura 2 - parameter distances of 7,223 tephritid DNA barcode sequences mostly extracted from BOLD Systems (www. boldsystems. org, as of Jan 2019), including 55 newly obtained Campiglossa sequences (names prefixed with Z). All 211 Campiglossa, Homoeotricha, and Dioxyna (regarded to be the genus Campiglossa, sensu lato, in this study) sequences were recovered as a monophyletic clade in this analysis. Putative species group names (in red) are marked on the respective branches.

Figure 2. MEGA X analysis produced a maximum-likelihood (ML) phylogram of the 76 selected Campiglossa (ingroup) and ten Tephritis (outgroup) DNA barcode sequences using the general time reversible model. The first number on each branch is the bootstrap support from ML analysis (pb); the second number represents posterior probability (pp) from Bayesian inference (BI). Samples in green letters were extracted from BOLD systems (www. boldsystems. org).

Figure 5. Genitalia of Campiglossa coei A epandrial complex, lateral view B epandrial complex, caudal view C glans and preglans of distiphallus D female postabdomen with aculeus and eversible membrane pulled out, ventral view E magnified view of aculeus tip F magnified view of oviscape and eversible membrane G spermatheca.

Figure 7. A-E Campiglossa paramelaena sp. nov. A male, lateral view B male, dorsal view C male wing D female, lateral view E female, dorsal view F female, wing G-K C. melaena G male, lateral view H male, dorsal view I male, wing J holotype male, wing K female, wing J, K Reproduced from Korneyev and Ovchinnikova (2004) with permission from Valery Korneyev.

Figure 9. Male genitalia of Campiglossa melaena A epandrial complex, lateral view B epandrial complex, caudal view C glans and preglans of distiphallus.