Cladosporium proteacearum Prasannath, Akinsanmi & R.G. Shivas, 2021

Lee, Wonjun, Kim, Ji Seon, Seo, Chang Wan, Lee, Jun Won, Kim, Sung Hyun, Cho, Yoonhee & Lim, Young Woon, 2023, Diversity of Cladosporium (Cladosporiales, Cladosporiaceae) species in marine environments and report on five new species, MycoKeys 98, pp. 87-111 : 87

Cladosporium proteacearum Prasannath, Akinsanmi & R.G. Shivas

Figs 2E , 3E

Holotype.

BRIP 72301a ( Prasannath et al. 2021).

Description.

Asexual morphology: Mycelium immersed, composed of septate, branched, pale brown, smooth to verruculose hyphae, 2-5 μm wide. Conidiophores macronematous and micronematous, arising laterally from hyphae, septate, erect to slightly flexuous, unbranched, up to 216 μm long, 2-4.5 μm wide, pale brown, verruculose. Conidiogenous cells integrated, terminal, cylindrical to subcylindrical, 18-59 × 2-4.5 μm, bearing up to three conidiogenous loci, slightly darkened and refractive. Ramoconidia 0-1(-2)-septate, subcylindrical to cylindrical, 9.2-38 × 2.3-4.7 μm [av. ( ± SD)24.1 ( ± 7.87) × 3.3 ( ± 0.58)], pale brown, smooth to verruculose. Conidia forming branched chains, with up to eight conidia in the terminal unbranched part, septate, pale brown, smooth to verruculose, slightly darkened and refractive hila. Small terminal conidia aseptate, ellipsoidal, 3.2-4.8 × 1.9-2.7 μm [av. ( ± SD) 3.9 ( ± 0.34) × 2.4 ( ± 0.2)]. Intercalary conidia 0(-1)-septate, ellipsoidal, oblong-ellipsoidal, 3.4-7.6 × 2.1-3.6 μm [av. ( ± SD) 4.8 ( ± 0.83) × 2.5 ( ± 0.32)]. Secondary ramoconidia 0-1-septate, oblong-ellipsoidal, ellipsoidal, subcylindrical, 4.5-25.6 × 1.9-4.5 μm [av. ( ± SD) 10.2 ( ± 4.3) × 3 ( ± 0.5)].

Cultural characters: Colonies on PDA 28-44 mm diam after 14 d at 25 °C, olive (2F7) to grayish yellow (2B4), reverse dark gray (1F1), velvety, raised, radially furrowed, wrinkled; margin white edge, undulated or lobate; aerial mycelia sparse, without prominent exudates, sporulation profuse, crack formed. Colonies on MEA 20-40 mm diam after 14 d at 25 °C, grayish green (29D7), reverse olive brown (4F8), velvety, powdery, raised, radially furrowed, umbonate, wrinkled; margin white, slightly undulate; aerial mycelia abundantly formed in protruding area (in the center), regular, without prominent exudates, sporulation profuse. Colonies on OA 39-46 mm diam after 14 d at 25 °C, wax white (2B3) to olive (2E5), reverse concolorous, floccose, flat, sometimes wrinkled; margin yellowish white (1A2), regular; aerial mycelia formed in spot, without prominent exudates, sporulation profuse. Colonies on SNA 40-41 mm diam after 14 d at 25 °C, olive yellow (2C7) to olive (1E4), reverse olive (2F5), powdery, flat; margin hyaline, regular; aerial mycelia sparse, without prominent exudates, sporulation profuse.

Habitat and distribution.

Isolated from plant material (Racames of Macadamia integrifolia ), sea sands, and seaweeds; Australia and Eastern, Southern, and Western Korean seaside and in Republic of Korea.

Additional cultures examined.

Republic of Korea. Ulsan, Ulju-gun, 35°23′00″N, 129°20′46″E, sea sand, Jan 2021, M.S. Park & Y.W. Lim (SFC20230103-M55, stored in a metabolically inactive state); Jeju-do, Hallim-eup, 33°23′53″N, 126°14′24″E, seaweed, Jul 2021, M.S. Park & Y.W. Lim (SFC20230103-M53, stored in a metabolically inactive state); Incheon, Ganghwa-gun, 37°35′18″N, 126°26′33″E, sea sand, Jan 2021, M.S. Park & Y.W. Lim (SFC20230103-M54, stored in a metabolically inactive state).

Notes.

This strain grows slower and forms less flat colonies on PDA compared to the holotype colonies (70 mm diam, flat) of C. proteacearum ( Prasannath et al. 2021). The characteristics of this species on MEA, OA, and SNA media were unavailable because the description on these media is insufficient ( Prasannath et al. 2021). Septa are visible in the secondary ramoconidia and intercalary conidia of this strain, but they have not been reported for the holotype ( Prasannath et al. 2021). To our knowledge, this is the first report of this species from the Republic of Korea and from marine environments.

Figures

Figure 2. Morphological characteristics of the new and unrecorded species on PDA, MEA, OA, and SNA media A Cladosporium lagenariiforme sp. nov. B Cladosporium maltirimosum sp. nov. C Cladosporium marinisedimentum sp. nov. D Cladosporium marinum sp. nov. E Cladosporium proteacearum F Cladosporium snafimbriatum sp. nov. " F " and " R " represent the front and the reverse sides of the culture plate, respectively. PDA: potato dextrose agar; MEA: malt extract agar; OA: oatmeal agar; SNA: synthetic nutrient-poor agar.

Figure 3. Morphological characteristics of conidial structures on SNA A Cladosporium lagenariiforme sp. nov. B Cladosporium maltirimosum sp. nov. C Cladosporium marinisedimentum sp. nov. D Cladosporium marinum sp. nov. E Cladosporium proteacearum F Cladosporium snafimbriatum sp. nov. Scale bars: 10 μm (A-F).