Cladosporium caprifimosum Iturrieta-Gonzalez , Dania Garcia , Gene, 2021

Iturrieta-Gonzalez, Isabel, Garcia, Dania & Gene, Josepa, 2021, Novel species of Cladosporium from environmental sources in Spain, MycoKeys 77, pp. 1-25 : 1

Cladosporium caprifimosum Iturrieta-Gonzalez, Dania Garcia, Gene sp. nov. Fig. 3

Etymology.

The name refers to goat dung, the substrate where the species was isolated (capra = goat and fimus = dung, with the adjectival Latin suffix -osus, indicating abundance or full or marked development).

Type.

Spain, Catalonia, Tarragona province, La Fatarella, from goat dung, Mar. 2017, I. Iturrieta-González, M. Guevara-Suarez & J. Guarro (holotype CBS H-24469; cultures ex-type FMR 16532, CBS 146918).

Description.

Mycelium in vitro superficial and immersed, composed of septate, branched, subhyaline, smooth to verruculose hyphae, 1-2 μm wide. Conidiophores dimorphic, micronematous or macronematous, arising from lateral or terminal hyphae, erect to slightly flexuous, non-nodulose, septate, branched or unbranched, 8-137 μm long, 2-4 μm wide, pale brown, slightly verrucose. Conidiogenous cells integrated, terminal, cylindrical, sometimes geniculate at the apex, 22-44 × 3-4 μm, bearing up to four conidiogenous loci, darkened and refractive. Ramoconidia aseptate, almost cylindrical, 10-24 × 2-4 μm [av. ( ± SD) 15.8 ( ± 3.4) × 3.1 ( ± 0.45)], olive to pale brown, smooth. Conidia forming branched chains, with up to five conidia in the terminal unbranched part, aseptate, olive to pale brown, smooth; small terminal conidia ellipsoidal to obovoid, 3-7 × 2-3.5 μm [av. ( ± SD) 5.7 ( ± 0.83) × 2.4 ( ± 0.43)]; intercalary conidia ellipsoidal to somewhat fusiform, 6-11.5 × 2-3 μm [av. ( ± SD) 7.8 ( ± 1.06) × 2.6 ( ± 0.39)]; secondary ramoconidia ellipsoidal to almost cylindrical, 9-14 × 2.5-3.5 μm [av. ( ± SD) 11.3 ( ± 1.6) × 2.9 ( ± 0.26)].

Culture characteristics

(14 d at 25 °C). Colonies on OA reaching 24-25 mm diam., dark green (30F8), flat, slightly dusty, aerial mycelium scarce, margin regular; reverse dark green (30F8) to black. On PDA attaining 34-35 mm diam., olive (3E6/3F4), slightly umbonate, radially folded, velvety, aerial mycelium scarce, margin slightly lobate; reverse dark green (30F8) to olive (3E4). On SNA reaching 25-26 mm diam., olive (3E8), flat, dusty, aerial mycelium scarce, margin regular; reverse dark green (30F8) to black.

Cardinal temperature for growth.

Optimum 20 °C, maximum 30 °C, minimum 5 °C.

Distribution.

Spain.

Notes.

Although C. caprifimosum clearly belongs to the C. cladosporioides species complex, our multi-locus analysis did not reveal any phylogenetic relationship with other species in the complex. It is represented by a single branch placed distance from other Cladosporium species (Fig. 1 ). Cladosporium caprifimosum differs from the other novel species proposed here mainly by its aseptate and smooth conidia.

Figures

Figure 1. Maximum likelihood (ML) tree obtained from the combined analysis of ITS, tef 1 and act sequences of 101 strains from the C. cladosporioides complex. The tree is rooted with C. sphaerospermum CBS 193.54 and C. longissimum CBS 300.96. Numbers on the branches represent ML bootstrap support values (MLBS) ≥ 70 %, followed by Maximum Parsimony bootstrap support values (PBS) ≥ 70 % and Bayesian posterior probabilities (PP) ≥ 0.95, lower values are indicate as " - ". Bold branches indicate MLBS / PBS / PP of 100 / 100 / 1. Names of species newly described are indicated in bold. Branch lengths are proportional to distance. T Ex-type strain. ET Ex-epitype strain.

Figure 3. Cladosporium caprifimosum (ex-type FMR 16532) a-c colonies on PDA, OA and SNA after 14 days at 25 ° C d-e conidiophores f ramoconidia and conidia. Scale bars: 10 mm (a-c); 10 μm (d-f).