Distoseptispora tectonae Doilom & K.D. Hyde, Fungal Diversity 80: 222 (2016)

Chen, Xue-Mei, Tang, Xia, Ma, Jian, Liu, Ning-Guo, Tibpromma, Saowaluck, Karunarathna, Samantha C., Xiao, Yuan-Pin & Lu, Yong-Zhong, 2024, Identification of two new species and a new host record of Distoseptispora (Distoseptisporaceae, Distoseptisporales, Sordariomycetes) from terrestrial and freshwater habitats in Southern China, MycoKeys 102, pp. 83-105 : 83

Distoseptispora tectonae Doilom & K.D. Hyde, Fungal Diversity 80: 222 (2016)

Fig. 4

Description.

Saprobic on dead twigs of Edgeworthia chrysantha . Sexual morph: Undetermined. Asexual morph: Colonies on natural substrate abundant, superficial, dark brown, hairy. Conidiophores 35-80 μm × 4-7.5 μm (x- = 58 × 5.5 μm, n = 20), macronematous, mononematous, simple, erect to slightly curved, solitary, pale brown to dark brown, cylindrical, 2-4-septate, slightly constricted at the septa, unbranched, thick-walled. Conidiogenous cells 6-10 μm × 3.5-6.5 μm (x- = 8 × 4.5 μm, n = 20), holoblastic, monoblastic, integrated, terminal, cylindrical, slightly tapering towards the apex, brown to reddish brown, percurrent. Conidia 190-255 μm × 9.5-16 μm (x- = 220 μm × 13 μm, n = 20), 5-16 μm (x- = 13 μm, n = 20) wide at the protruding truncate base; 4.5-8 μm (x- = 6.5 μm, n = 20) wide in the tapering part, acrogenous, solitary, obclavate, elongate, rostrate, straight or curved, tapering towards the apex, 9-39-distoseptate, olivaceous-green when young, dark reddish brown at maturity, verrucose.

Culture characteristics.

Conidia germinating on PDA within 24 h, colonies circular, dense, umbonate, spreading, fluffy. The surface is slightly rough with reddish-gray mycelium, colonies somewhat raised in the middle, and with a filiform edge. The reverse side is dark gray with a circular, pale reddish-gray edge, not pigmented.

Material examined.

China, Guizhou Province, Guiyang City, Guiyang Medicinal Botanical Garden , on dead twigs of Edgeworthia chrysantha , 20 August 2022, Xia Tang, JX 30 (GZAAS 22-2046), living culture, GZCC 22-2046 .

Known host and distribution.

Tectona grandis (Thailand, Hyde et al. 2016), on dead stems (Thailand, Sun et al. 2020), on dead, submerged, decaying wood of unidentified plants (China & Thailand, Luo et al. 2019; Dong et al. 2021; Zhang et al. 2022), and dead twig and branch of Edgeworthia chrysantha (China, this study).

Notes.

Distoseptispora tectonae was first isolated from a dead twig of Tectona grandis in Thailand ( Hyde et al. 2016). Since then, this species has been identified in various countries on different substrates and hosts ( Hyde et al. 2016; Sun et al. 2020; Dong et al. 2021; Zhang et al. 2022). In the phylogenetic tree (Fig. 1 ), our new isolate forms a close lineage to D. tectonae (GZCC 22-2046) with statistical support of 98% ML and 0.96 PP. Based on pairwise nucleotide comparisons of ITS, LSU, tef 1-α, and rpb 2, our new isolate diverges from D. tectonae (MFLUCC 12-0291, ex-type) by 6/554 bp (1%) for ITS, 1/852 bp (0.01%) for LSU, 0/980 bp (0%) for tef 1-α, and 2/899 bp (0.2%) for rpb 2. In addition, the morphological characteristics of our isolate match well with the holotype description of D. tectonae ( Hyde et al. 2016). This study reports a new host record of Distoseptispora tectonae on dead twigs of Edgeworthia chrysantha in China.

Figures

Figure 1. Phylogenetic tree generated from ML analysis based on a combination of LSU, ITS, tef 1 - a, and rpb 2 sequence data. Bootstrap support values of ML and MP equal to or greater than 75 %, and PP value equal to or greater than 0.95 are given near the nodes as ML / PP / MP. The tree is rooted with Aquapteridospora aquatica (MFLUCC 17 - 2371). Ex-type strains are indicated by the superscript T. The new collections are in bold red text.

Figure 4. Distoseptispora tectonae (GZAAS 22 - 2046) a, b colonies on substrate c, d conidiophores and conidia e, f conidiophores g-k conidia l germinated conidium m, n colonies on PDA (m from front n from reverse) Scale bars: 50 μm (c, d, g-l); 20 μm (e, f).