Celoporthe paradisiaca S.F. Chen & Marinc., 2020

Roux, Jolanda, Kamgan Nkuekam, Gilbert, Marincowitz, Seonju, van der Merwe, Nicolaas A., Uchida, Janice, Wingfield, Michael J. & Chen, ShuaiFei, 2020, Cryphonectriaceae associated with rust-infected Syzygium jambos in Hawaii, MycoKeys 76, pp. 49-79 : 49

Celoporthe paradisiaca S.F. Chen & Marinc. sp. nov. Fig. 5

Etymology.

The species name refers to the fact that Hawaii, where the holotype of this fungus was collected, is regarded as a paradise by travellers.

Types.

Holotype: USA, Hawaii, O’ahu Island, Ho’omaluhia, isolated from bark of Psidium cattleianum , 24 July 2012, J. Roux (PREM 63205; Ex-type culture CMW38360 = CBS 147169); GenBank accession numbers KJ027498 (ITS), KJ027474 (BT1), KJ027483 (TEF1). Paratype: Hawaii, O’ahu Island, Waimea Valley Botanical Gardens, isolated from bark of Syzygium jambos , 23 July 2012, J. Roux (PREM 63206; living culture CMW38368 = CBS 147170).

Sexual morph.

Not observed.

Asexual morph.

Produced after two months on Eucalyptus stem sections placed on water agar. Conidiomata superficial or with base embedded, singular or gregarious, pulvinate or conical with or without necks, often covered with mycelia, unilocular or multilocular, convoluted, 354-841 µm long, 185-654 µm wide. Conidiomatal wall outer or inter-locular stratum prosenchymatous; inner fertile layers pseudoparenchymatous, composed of several layers of flattened, thick-walled, pigmented cells, 8-19 µm thick. Paraphyses present, rarely observed. Conidiophores produced along inner layer of locule, simple or scarcely branched from basal cell, 8-11 µm long. Conidiogenous cells enteroblastic, lageniform, tapering towards apex, 5-11 × 1-2 µm. Conidia hyaline, oblong, straight or occasionally curved, 3-4 × 1-1.5 (3.2 ± 0.3 × 1.2 ± 0.07) µm.

Culture characteristics.

Colonies on 2% MEA, when young, showing circular growth with smooth edges, above white, reverse pale to dark brown (30 °C) and yellow (25 °C); with age, above becoming brown and reverse dark yellow; optimal growth at 30 °C (7.7 mm/d), followed by 25 °C (7.0 mm/d) and 20 °C (4.6 mm/d), minimal growth at 35 °C (0.1 mm/d), no growth at 5 °C; mycelia fluffy, density-sparse in centre, becoming thicker towards the edge, aerial hyphae more abundant at 25 °C than at 30 °C when young.

Habitat.

On/in bark of Psidium cattleianum and Syzygium jambos

Distribution.

Hawaii, USA

Notes.

Celoporthe paradisiaca is morphologically similar to its phylogenetically closest relatives, Cel. hawaiiensis and Cel. guangdongensis , but can be differentiated from them by DNA sequences. In the ITS, BT1 and TEF1 datasets, Cel. paradisiaca differs from Cel. hawaiiensis by 6, 3 and 3 base pairs and from Cel. guangdongensis by 7, 4 and 2 base pairs, respectively (Tables 6 , 7 ).

Figures

Figure 5. Micrographs of Celoporthe paradisiaca sp. nov. (holotype: PREM 63205, ex-holotype CBS 147169 = CMW 38360) A culture morphology on 2 % MEA at 25 ° C and 30 ° C at 8 and 34 days B conidiomata produced on Eucalytpus stem sections on water agar C, D vertical section of conidioma E inner wall of conidioma F conidiomatal walls (ps, pseudoparenchymatous inner wall; pr, prosenchymatous outer or interlocular wall) G conidiogenous cells H conidia. Scale bars: 1 mm (B); 100 µm (C, D); 10 µm (E-H).