Pseudosinella hartnerae Winkler & Fiera, 2021

Winkler, Daniel, Nemeth, Marton Tamas & Fiera, Cristina, 2021, A new species of Pseudosinella Schaeffer, 1897 (Collembola, Entomobryidae) from Hungary and Romania, with redescription of the related species Pseudosinella huetheri Stomp, 1971, ZooKeys 1063, pp. 121-137 : 121

Pseudosinella hartnerae Winkler & Fiera sp. nov.

Figures 1 , 2 , 3 , 4 , 5

Type material.

Holotype: Hungary. ♀ on slide (Nr. HNHM collpr-896), Bátorliget, Szabolcs-Szatmár-Bereg county, 161 m a.s.l., 47°46'11"N, 22°16'19"E, from litter, hand collecting, 8 Oct. 2017, leg. D. Winkler. Paratypes: Two ♀ and one ♂ on slide (Nr. HNHM collpr-896); two ♀ and one specimen with sex not visible (Nr.: HNHM collpr-897); two ♀ (Nr. WD-coll-113 and WD-coll-114); same data as holotype. The holotype and four paratypes are deposited in the Hungarian Natural History Museum (HNHM), Budapest. Two paratypes are preserved in the first author’s collection at the University of Sopron, Faculty of Forestry, Sopron, Hungary; one paratype is kept in C. Fiera’s collection (IBB).

Other material.

Romania. Seven specimens (one ♂ on slide, Nr.: IBB Coll-12544; and six specimens of sex not determined in ethyl alcohol, vial Nr. IBB-26), Lake Saint Ana , Harghita County , Romania, 984 m a.s.l., 46°7'33"N, 25°53'28"E, 2 Nov. 2017, mixed forest with beech and fir, from litter, hand collecting, leg. C. Fiera.; 4 specimens (one ♀ on slide, Nr. IBB Coll-12545; and three specimens of sex not determined in ethyl alcohol, vial Nr. IBB-26), Mohoş peat bog, 1050 m a.s.l., 46°8'6"N, 25°53'59"E, 2 Nov. 2017, Scots pine forest, from peat moss Sphagnum , hand collecting, leg. C. Fiera. Preserved in the last author’s collection (IBB) .

Diagnosis.

5+5 ocelli. Colour bluish-grey. Labial chaetotaxy M1m2rel1L2, r vestigial. Dorsal macrochaetae formula R0R1R2001/00/0101+2. Abd II chaetotaxy: paBq1q2.Abd IV accessory chaeta s, anteriorly to trichobothrial complex, absent. Antennae and legs without scales. Unguis inner side with two paired basal teeth and one unpaired tooth. Unguiculus outer lamella smooth.

Description.

Habitus (Fig. 1 ). Body length (without head and furca) 1.01-1.27 mm (holotype: 1.08 mm). Colour: Head, antennae, trunk and legs bluish-grey, blue shades also on manubrium and ventral tube. Eye patches dark blue.

Head. With 5+5 eyes (ABCDH, with H only slightly smaller). Dorsal cephalic main macrochaetae formula R0R1R2P (according to AMS notation A0, A2, A3 and Pa5) (Fig. 2A ). Maximum number of macrochaetae “An” on head 9+9. Antennal length to head diagonal length (measured from cervical edge to apex of labrum) ratio 1.2-1.4 (holotype: 1.3). Relation of antennal segments I-IV as 1: 1.7: 1.5: 2.9 (holotype). Ant III sensillary organ composed of two rod-like sensilla partially behind a cuticular fold, guarded by three sensilla, one of them short, with spiny morphology (Fig. 2B ). Ant IV without apical bulb. Clypeus with eleven subequal ciliated chaetae (three in row pf, four in row ft, two in row l1 and two in row l2) (Fig. 2C ). Arrangement of chaetae on labrum 4/554, prelabral chaetae smooth; first (p), second (m) and apical (a) rows of labral chaetae also smooth, chaetae of p and m series about the same in size, not enlarged, a1-a2 thicker but not enlarged; labral edge with no differentiated papillae (Fig. 2D ). Outer maxillary palp with two smooth chaetae and three smooth main sublobal hairs. Lateral process (sensu Fjellberg 1999) on papilla E finger-shaped, barely reaching the top of papilla (Fig. 2E ). Labial anterior row with five smooth chaetae (a1-a5); formula of basal row M1m2rel1L2 with M1 and L2 ciliated and all other chaetae smooth (Fig. 2F ). Chaeta r short (ratio of r/m2 0.2-0.3). Ventral chaetotaxy with about 15 ciliate chaetae, postlabial formula 4 (G1-4), 2 (X, X4), 4 (H1-4), and 2 (J1-2) chaetae; b.c. present (Fig. 2F ).

Body. Body dorsal macrochaetae from Th II to Abd IV 00/0101+2. Mesothorax without macrochaetae. Two anterolateral S-chaetae (al and ms) present. Th III without Mac, anterolateral sensillum al present. Abd I with lateral S-microchaeta (ms). Chaetotaxy of Abd II-III as in Fig. 3A, B . Abd II chaetotaxy between two dorso-medial trichobothria paBq1q2 using Gisin’s symbols ( Gisin 1967); following Szeptycki’s (1979) notation p=a2p, a=a2, B=m3, q1=m3e and q2=p4. Chaeta a as ciliated mic. Abd III chaeta d3 present. Chaetotaxy and trichobothrial complex on Abd IV as in Fig. 4A, B . Mac B5, B6, C1, E2, E3, F1 and F2 broader with broad sockets, D2, D3, De3, E4, E4p, E4p2, F3, F3p, Fe4, Fe5, T6 and T7 thinner with smaller sockets. Abd IV with five fan-shaped chaetae (D1, a, m, pe and pi) associated with two trichobothria. Accessory chaeta s, associated with trichobotrium T2, absent. Nine S-chaetae (as, ps, and seven long dorsal S-chaetae) present. Dorsal chaetotaxy of Abd V as in Fig. 4C . Three S-chaetae (as, acc.p4 and acc.p5) typical for Pseudosinella present. Legs without scales. Trochanteral organ with up to 12 smooth spiny chaetae forming a V-shaped pattern (Fig. 5A ). Unguis and unguiculus as in Fig. 5B . Unguis with subequal paired basal teeth at 35% from inner edge, and with a median unpaired inner tooth at 60% from inner edge, apical tooth absent. Outer side with paired lateral teeth and a short external tooth. Unguiculus lanceolate, external lamella smooth. Tibiotarsal tenent hair clavate, supraempodial chaeta on tibiotarsus III smooth and acuminate. Ratio of supraempodial chaeta / unguiculus around 0.9. Ventral tube without scales; with 6+6 subequal ciliated chaetae on anterior side and 4+4 subequal ciliated chaetae on posterior side; lateral flap with 1 ciliated and a maximum of 6 smooth chaetae (Fig. 5C ). Manubrium ventrally with scales and 2+2 terminal ciliated chaetae. Manubrial plate with 2 larger inner chaetae and 2 chaetae external to the 2 pseudopores (Fig. 5D ). Length of not ringed terminal dens about 4 times the length of mucro. Mucro with distal tooth equal to anteapical one; basal spine reaching tip of anteapical tooth (Fig. 5E ). Ratio manubrium/dens/mucro as 16:15:1.

Ecology and distribution.

The type locality ( Bátorliget Pasture Nature Conservation Area, Szabolcs-Szatmár-Bereg county, Hungary) of Pseudosinella hartnerae sp. nov. is a special relict mire and forest area with high biodiversity. The new species was collected from the upper layer and litter of a forest clearing with pioneer vegetation including silver birch ( Betula pendula ) trees. Specimens in Romania were collected from litter in the surrounding forest of the volcanic lake Saint Ana, and from peat mosses in the nearby Mohoș bog. Both Romanian sites are located in the Mohoş Nature Reserve, Harghita County. This new Pseudosinella is a phytodetriticolous, bryophilous and hygrophilous species.

Etymology.

The name of the new species is dedicated to former zoologist colleague and friend Dr. Anna Fenyősiné Hartner (1965-2006), an excellent specialist in myrmecology.

Figures

Figure 1. Pseudosinella hartnerae sp. nov. Habitus. Scale bar: 0.5 mm.

Figure 2. Pseudosinella hartnerae sp. nov. A head, dorsal chaetotaxy (left side) B antennae, Ant III with sensillar organ C clypeus, clypeal chaetae D labrum E labial papilla E with lateral process F labial triangle (right side) and ventral cephalic groove with labial and postlabial chaetotaxy. Scale bars: 0.05 mm (A); 0.03 mm (B-D, F); 0.01 mm (E).

Figure 3. Pseudosinella hartnerae sp. nov. Abdomen dorsal chaetotaxy: A Abd II (left side) B Abd III (left side). Scale bar: 0.03 mm (A, B).

Figure 4. Pseudosinella hartnerae sp. nov. Abdomen dorsal chaetotaxy: A Abd IV (left side) B Abd IV trichobothrial complex (left side) C Abd V. Scale bars: 0.05 mm (A); 0.02 mm (B); 0.03 mm (C).

Figure 5. Pseudosinella hartnerae sp. nov. A trochanteral organ B leg III unguis and unguiculus C ventral tube anterior view (right side) and posterior view (left side) D manubrial plate E mucro and apical part of dens. Scale bars: 0.02 mm (A, B, D, E); 0.03 mm (C).