Sporothrix silvicola R. Jankowiak & P. Bilanski, 2023

Bilanski, Piotr, Jankowiak, Robert, Solheim, Halvor, Fortuna, Pawel, Chyrzynski, Lukasz, Warzecha, Paulina & Taerum, Stephen Joshua, 2023, Soil-borne Ophiostomatales species (Sordariomycetes, Ascomycota) in beech, oak, pine, and spruce stands in Poland with descriptions of Sporothrix roztoczensis sp. nov., S. silvicola sp. nov., and S. tumida sp. nov., MycoKeys 97, pp. 41-69 : 41

Sporothrix silvicola R. Jankowiak & P. Bilanski sp. nov.

Fig. 7

Etymology.

Referring to the Latin silva (forest) and - cola (inhabiting), with reference to its woody habitat.

Diagnosis.

Sporothrix silvicola differs from the phylogenetically closely related species S. dimorphospora and S. roztoczensis with respect to its conidia dimensions.

Type.

Poland, Lubelskie Province, Józefów, from wood buried in soil under 43-year- old managed Pinus sylvestris forest, July 2015, Ł. Chyrzyński, (O-F- 259451 holotype, culture ex-type CBS 149241) .

Description.

Sexual morph not observed. Asexual structures produced on sterilized Scots pine twigs placed on the surface of malt agar in Petri dishes. Conidiophores hyaline, one-celled, micronematous, simple, either borne on vegetative hyphae or on upright hyphae. Conidiogenous cells blastic, cylindrical, terminal, lateral or intercalary, straight or curved, constricted at the base and tapering towards the apex, (2.2-)11.6-35.6(-60.5) μm long, (0.7-)1-1.5(-1.8) μm wide at the base, apical part forming conidia by sympodial proliferation on swollen cluster of conidium-bearing denticles, (1.4-)2.6-4.4(-5.5) μm long and (1.5-)2.1-3.4(-4.1) μm wide, denticles often arise below the swollen cluster. Conidia of two types: 1) abundant in cultures hyaline, unicellular, smooth, guttuliform, ellipsoid, pointed at the base, sometimes curved (3.2-)3.6-6.4(-10.4) × (1.4-)1.6-2.5(-3.6) μm, formed directly on denticles; 2) abundant in cultures, subhyaline to lightly pigmented, unicellular, smooth, subglobose to broadly ellipsoidal, sometimes pointed at the base, (2.6-)3.1-4.1(-4.8) μm × (1.4-)2.1-3.4(-3.9) μm diam., formed singly, on lateral or intercalary conidiogenous cells or denticles directly emerging from vegetative hyphae.

Culture characteristics.

Colonies with optimal growth at 20 °C on 2% MEA reaching an average of 32 mm ( ± 1.86 mm) after 14 days, with radial growth rate 0.89 ( ± 0.07) mm/d, growth somewhat slower at 15 °C (26.6 mm diameter), no growth at 30 and 35 °C; dark grey to olivaceous with white margins, floccose, lanose with abundant white aerial hyphae, flat, growing in a circular pattern with entire margins.

Distribution.

Known only from the type location (Poland).

Additional specimen examined.

Poland, Lubelskie Province, Józefów, from wood buried in soil under 93-year old managed Pinus sylvestris forest, July 2015, Ł. Chyrzyński (O-F-259450, culture CBS 149240).

Notes.

This species is phylogenetically distinct from the other Sporothrix species based on the TUB 2, TEF 1, and CAL sequences. The morphological differences between S. dimorphospora and S. roztoczensis are described in the section treating S. roztoczensis . Sporothrix silvicola had identical ITS and TUB 2 sequences as two isolates of ' S. inflata 2' (CBS 156.72, CBS 427.74) obtained from greenhouse soil and isolated from Lilium sp. in the Netherlands ( Aghayeva et al. 2005; de Beer et al. 2016).

Figures

Figure 7. Sporothrix silvicola sp. nov. (CBS 149241) a, b conidiogenous cell with an inflated cluster of denticles at the apex c conidia d globose conidia arising on conidiophore e globose conidia arising on denticles formed directly from hyphae f fourteen-day-old culture on MEA. Scale bars: 10 μm.