Limosilactobacillus fastidiosus, Li & Cheng & Zheng & Liu & Quevedo & Li & Roos & Gänzle & Walter, 2021

Li, Fuyong, Cheng, Christopher C., Zheng, Jinshui, Liu, Junhong, Quevedo, Rodrigo Margain, Li, Junjie, Roos, Stefan, Gänzle, Michael G. & Walter, Jens, 2021, Limosilactobacillus balticus sp. nov., Limosilactobacillus agrestis sp. nov., Limosilactobacillus albertensis sp. nov., Limosilactobacillus rudii sp. nov. and Limosilactobacillus fastidiosus sp. nov., five novel Limosilactobacillus species isolated from the vertebrate gastrointestinal tract, and proposal of six subspecies of Limosilactobacillus reuteri adapted to the gastrointestinal tract of specific vertebrate hosts, International Journal of Systematic and Evolutionary Microbiology (004644) 71 (2), pp. 1-21 : 17

DESCRIPTION OF LIMOSILACTOBACILLUS FASTIDIOSUS SP. NOV.

Limosilactobacillus fastidiosus (fas.ti.di.o′ sus. L. masc. adj. fastidious, fastidious, referring to the fastidious growth requirements of the type strain).

Cells are Gram-positive, non-motile, non-spore-forming, catalase-negative and heterofermentative. Cells are rodshaped, measuring 0.9–3.0×0.6–0.9 µm. Colonies of the type strain WF-MO7-1 T on MRS agar plate incubated at the anaerobic condition at 37 °C for 2 days are whitish, opaque, raised, circular and entire, with a diameter of 1.2–2.2 mm; no colony appears on MRS agar plate incubated at the aerobic condition at 37 °C for 2 days. D-Lactate,L-lactate and gas are produced from glucose fermentation by the type strain WF-MO7-1 T. No growth occurs in 96-well microplate at 15, 30, 37 or 45 °C; in Falcon 15 ml tubes, cell growth occurs at 30, 37 and 45 °C (optimum) but not at 15° C. Growth occurs at pH 4.5–7.5 in MRS broth. The most abundant fatty acids of WF-MO7-1 T are C 16:0, summed feature 7 (combination of C 19:1 ω 6 c and/ or C 19:0 cyclo ω 10 c) and C 18:1 ω 9 c. Acid is produced from L-arabinose and aesculin; acid production from D-galactose, D-glucose,D-fructose, maltose, lactose, melibiose and raffinose is strain-specific; acid is not produced from D-ribose, D-xylose,D-mannose, methylα- D-glucopyranoside, sucrose, potassium gluconate, glycerol, erythritol, D-arabinose, L-xylose,D-adonitol, methyl β -D-xylopyranoside,L-sorbose, L-rhamnose, dulcitol, inositol, D-mannitol,D-sorbitol, methyl α- D-mannopyranoside, N -acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, trehalose, inulin, melezitose, starch, glycogen, xylitol, gentiobiose, turanose, D-lyxose, D-tagatose,D-fucose,L-fucose,D-arabitol,L-arabitol, potassium 2-ketogluconate or potassium 5-ketogluconate. The cellwall peptidoglycan of WF-MO7-1 T contains the amino acids alanine (Ala), glutamic acid (Glu), aspartic acid (Asp) and ornithine (Orn), with the molar ratio 1.5 (Ala):0.9 (Asp):1.0 (Glu):1.1 (Orn), suggesting the cell-wall peptidoglycan type A4α L-Orn–D-Asp by [ 38]. The DNA G+C content of WF-MO7-1 T is 39.1mol%.

The type strain is WF-MO7-1 T (=DSM 110576 T =LMG 31630 T), which was isolated from the jejunum of root vole ( Microtus oeconomus ) caught in the Vilnius area in Lithuania [ 13].