Neophasis spp

Kremnev, Georgii, Gonchar, Anna, Krapivin, Vladimir, Uryadova, Alexandra, Miroliubov, Aleksei & Krupenko, Darya, 2021, Life cycle truncation in Digenea, a case study of Neophasis spp. (Acanthocolpidae), International Journal for Parasitology: Parasites and Wildlife 15, pp. 158-172 : 167-168

3.4. Phylogenetic position of Neophasis spp

Concatenated SSU and LSU dataset comprised 24 sequences and yielded 2977 characters, including gaps. Phylogenetic trees inferred with ML and Bayesian approaches revealed the same topology. The

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Bayesian tree is presented in Fig. 8 ; ML bootstrap support values are additionally mapped onto it next to the posterior probabilities (PP) values for all the ML-supported nodes.

There are three well-supported major groups on the tree: Brachycladiidae + Acanthocolpidae clade A and Acanthocolpidae clade B. This is in agreement with previous studies ( Bray et al., 2005; Curran and Pulis, 2014; Fraija-Fern´andez et al., 2015; Kremnev et al., 2020). Two Neophasis spp . group together, with high PP and bootstrap support values, within the Acanthocolpidae clade A. This clade also includes Tormopsolus orientalis Yamaguti, 1934 and Pleorchis spp. , but the branching order of these three lineages is unresolved.

Figures

Fig. 8. Phylogenetic position of Neophasis oculata and N. anarrhichae based on the concatenated 18S and 28S rDNA sequence data, inferred with Bayesian inference. Newly generated sequences are indicated in bold. Posterior probabilities are printed at nodes, followed by bootstrap values for the nodes that were also supported in the tree inferred with Maximum likelihood method. Scale bar shows the substitution rate. GenBank accession numbers for the 18S and 28S rDNA sequences are listed in the Supplementary Table S1.