Cladosporium yunnanensis H.W. Shen, Y.X. Xu, H.Y. Su & Z.L. Luo, 2021

Xu, Yue-Xin, Shen, Hong-Wei, Bao, Dan-Feng, Luo, Zong-Long, Su, Hong-Yan & Hao, Yu-E, 2021, Two new species of Cladosporium from leaf spots of Paris polyphylla in north-western Yunnan Province, China, Biodiversity Data Journal 9, pp. 77224-77224 : 77224

Cladosporium yunnanensis H.W. Shen, Y.X. Xu, H.Y. Su & Z.L. Luo sp. nov.

Materials

Type status: Holotype. Occurrence: recordedBy: Yue-Xin Xu; Taxon : scientificName: Cladosporium yunnanensis; kingdom: Fungi ; phylum: Ascomycota ; class: Dothideomycetes ; order: Capnodiales ; family: Cladosporiaceae ; genus: Cladosporium ; Location : locationRemarks: China, Yunnan Province, Dali , on diseased leaves of Paris polyphylla, 2 October 2020; Event: day: 2020; habitat: leaf spots of Paris polyphylla; Record Level: collectionID: 1CL JD 5-1-4; collectionCode: Y-23

Description

Asexual morph: hyphomycetous (Fig. 2 ). Mycelium superficial and immersed, composed of septate, branched, subhyaline, smooth-walled, 1-3 μm wide. Conidiophores macronematous, 127-190 × 4-6 μm (x̄ = 158.2 × 5.1 μm, n = 15), solitary or in small loose groups, erect to slightly flexuous, non-nodulose, sometimes subnodulose at the uppermost apex, unbranched, 0-6 septate, sometimes slightly constricted at septa, pale brown, smooth, sometimes somewhat irregularly rough-walled or verruculose. Conidiogenous cells terminal and intercalary, loci crowded at the apex forming clusters of pronounced scars, 1-2 conidiogenous loci formed at about the same level, loci often situated at lateral shoulders due to sympodial proliferation, loci 1-2 μm diam. Conidia solitary or in short unbranched chains, straight to slightly curved, cylindrical-oblong, 7-19 × 5-7 μm (x̄ = 13.2 × 5.7 μm, n = 30), 0-3 septate, sometimes slightly constricted at the septa, pale to pale medium olivaceous-brown. Sexual morph: Undetermined.

Culture characteristics: Colonies on PDA attaining 25 mm diam. after 7 d, 45 mm diam. after 14 d and covering the whole Petridish after 30 d, dark green to olive green, velvety, furrowed; reverse dark green to black.

Material examined: China, Yunnan Province, Dali, on diseased leaves of Paris polyphylla , 2 October 2020, Y.X. Xu, Y-23. (KUN-HKAS 121704, holotype), ex-type living culture CGMCC 3.20622 = KUNCC 21-10712

Etymology

" Cladosporium yunnanensis " refers to Yunnan Province, China, where the species was collected.

Distribution

China, Yunnan Province, Dali, on diseased leaves of Paris polyphylla

Notes

Based on the multi-locus phylogenetic analysis (Fig. 1 ), Cladosporium yunnanensis grouped in a well-supported clade, together with C. cladosporioides and C. magnoliigena . However, the genetic distance allows it to be considered a distinct species within the clade (Fig. 1 ). Morphologically, C. yunnanensis has much shorter conidiophores than C. cladosporioides (up to 190 μm vs. up to 350 μm), but longer than C. magnoliigena (up to 190 μm vs. up to 150 μm). Moreover, the new species differs from C. cladosporioides by the smaller conidiogenous cells (7-19 × 5-7 μm vs. 4-18 × 2-5 μm), but larger than C. magnoliigena (7-19 × 5-7 μm vs. 4-18 × 2-5 μm) ( Bensch et al. 2012, Jayasiri et al. 2019). The BLAST analysis of TEF1-α and ACT shows that C. yunnanensis (KUN-HKAS 121704) is different from C. cladosporioides (CBS 112388) by 16 and 10 nucleotide differences, respectively and the comparison of TEF1-α between C. yunnanensis (KUN-HKAS 121704) and C. magnoliigena (CBS 140463) reveals 33 nucleotide differences.

Figures

Figure 1. Maximum Likelihood (ML) tree obtained from the combined analysis of ITS, TEF 1 - α and ACT sequences of 161 strains from Cladosporium. The tree is rooted with Toxicocladosporium irritans (CBS 185.58) and T. protearum (CBS 126499). Numbers on the branches represent ML bootstrap support values (MLBS) ≥ 70 %, followed by Bayesian posterior probabilities (PP) ≥ 0.95, lower values are indicated as " - ". Names of species newly described are indicated in red and ex-type strains and reference specimens are indicated in bold. Branch lengths are proportional to distance.

Figure 2. Cladosporium yunnanensis (KUN-HKAS 121704, holotype). a Colonies; b-c Conidiophores; d-g Conidiogenous cells with conidia; h-m Conidia; n Germinating conidium; o Culture on PDA from above and reverse. Scale bars: b-d = 20 μm; e-k = 15 μm; l = 30 μm.