Actinomadura harenae, Hu, Jiangmeihui, Han, Chuanyu, Yu, Bing, Zhao, Junwei, Guo, Xiaowei, Shen, Yue, Wang, Xiangjing & Xiang, Wensheng, 2020

Hu, Jiangmeihui, Han, Chuanyu, Yu, Bing, Zhao, Junwei, Guo, Xiaowei, Shen, Yue, Wang, Xiangjing & Xiang, Wensheng, 2020, Actinomadura harenae sp. nov., a novel actinomycete isolated from sea sand in Sanya, International Journal of Systematic and Evolutionary Microbiology 70 (2), pp. 766-772 : 771

DESCRIPTION OF ACTINOMADURA HARENAE SP. NOV.

Actinomadura harenae (ha.re′ nae. L. gen. n. harenae of sand).

The cells of the strain are found to be Gram-positive and aerobic. White aerial mycelium is produced abundantly and differentiates into flexuous or straight spore chains consisted of cylindrical spores (0.4–0.6×0.8–1.0 µm), the spore surface is rough on ISP 3 medium. Good growth on ISP 1 agar, ISP 2 agar, ISP 3 agar, ISP 6 agar, ISP 7 agar, NA and BA media; moderate growth on ISP 4 agar; and poor growth on ISP 5 agar and CA media. The colony colours vary from brilliant yellow to grey-green yellow on agar media and the strain produces moderate olive brown soluble pigment on ISP 7 medium. Strain NEAU-Ht49 T grows at 10–45 °C (optimum, 28 °C), pH 5–10 (pH 7) and NaCl tolerance of 0–3% (0 %). Positive for decomposition of cellulose, hydrolysis of aesculin and Tweens (40 and 80) and production of urease, but negative for hydrolysis of starch and Tween 20, liquefaction of gelatin, peptonization and coagulation of milk, production of H 2 S, and reduction of nitrate. Dulcitol, D-fructose, D-glucose, meso-inositol, maltose, D-mannitol, D-mannose, L-rhamnose and sucrose are utilized as sole carbon sources, but not L-arabinose, D-galactose, lactose, raffinose, D-ribose, D-sorbitol or D-xylose. L-Alanine, L-arginine, L-asparagine, L-aspartic acid, L-glutamic acid, L-glutamine, glycine, L-proline, L-serine, L-threonine and L-tyrosine are utilized as sole nitrogen sources, but not creatine. The diagnostic diamino acid of the cell wall is meso-diaminopimelic acid. Whole-cell sugars contain glucose, madurose, mannose and ribose. The polar lipid profile consists of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside and two unidentified lipids. The menaquinones are MK-9(H 6), MK-9(H 4) and MK-9(H 8). Major fatty acids are C 16:0, C 18:1 ω9 c, 10-methyl C 18:0 and iso- C 16:0 (>8%). The G+C content of the DNA of the type strain is 72.1 mol%.

The type strain is NEAU-Ht49 T (=CGMCC 4.7499 T =JCM 32659 T), isolated from sea sand collected from Wuzhizhou island in Sanya , Hainan Province, PR China. The GenBank/ EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain NEAU-Ht49 T is MK203829 . This Whole Genome Shotgun project has been deposited at DDBJ/ENA/ GenBank under the accession number RFFG00000000. The version described in this paper is version RFFG01000000.1 .