Periconia byssoides Pers., Syn.

Madagammana, Ashani D., Phukhamsakda, Chayanard, Bhunjun, Chitrabhanu S., Rathnayaka, Achala R., Bhat, D. Jayarama, Alotibi, Fatimah & Hyde, Kevin D., 2024, Novel Hosts, Geographical Records, and A Global Checklist of molecularly confirmed Periconia species, Phytotaxa 674 (1), pp. 18-54 : 24-27

Periconia byssoides Pers., Syn. meth. fung. (Göttingen) 2: 686 (1801)

Index Fungorum number: IF 144538, Figure 3

Saprobic on dead twigs of Tephrosia cinerea (L.) Pers. Sexual morph: Undetermined. Asexual morph: Colonies superficial on the host substrate, effuse, hairy, dark brown. Conidiophores 200–350 × 10–12 μm wide (x̄ = 288 × 11.5 µm, n = 10), macronematous, mononematous, solitary, arising from an inconspicuous stromatic base, straight or slightly flexuous, 2−4 septate, dark brown to black at base, pale brown towards apex, with spherical conidial head, slightly constricted near conidial head and knot-like near the base, smooth or slightly rough-walled. Conidiogenous cells 5–9 × 7–11 μm (x̄ = 8 × 9 μm, n = 10), polyblastic, discrete, terminal, subglobose to ellipsoidal, light brown, located at nodose apices of conidiophores. Conidia 13–17 × 12–18 μm (x̄= 15 × 14.7 μm, n = 10), globose to subglobose, orangish brown to brown, echinulate or verruculose, solitary or catenate.

Culture characters: Colonies on PDA reaching 50 mm diam. after 14 days at 25 °C, irregular with smooth margin, slightly woolly flattened mycelia, circular, cottony, hairy at the margin, white; pale yellow at the margin and mud yellow at the middle in reverse.

Material examined: Thailand, Chiang Rai, Tha Sut sub district, Mueang District, on recently dead twigs of Tephrosia cinerea (L.) Pers. ( Fabaceae ), 28 January 2023, collected and identified by Ashani Madagammana, AD 0408 (MFLU 23–0453); living cultures MFLUCC 23-0294.

Known host and distribution: Angelica sylvestris L. ( Apiaceae ), Conium maculatum L. ( Apiaceae ), Heracleum sosnowskyi Manden. ( Apiaceae ) in Lithuania ( Markovskaja and Kačergius 2014); Ampelopsis brevipedunculata (Maxim.) Trautv. ( Vitaceae ), Benthamidia japonica (Siebold & Zucc.) H.Hara ( Cornaceae ), Prunus verecunda (Koidz.) Koehne ( Rosaceae ) in Japan ( Tanaka et al. 2015); Celtis formosana Hayata ( Cannabaceae ), Macaranga tanarius (L.) Müll.Arg. ( Euphorbiaceae ) in Taiwan region in China ( Tennakoon et al. 2021); Imperata cylindrica (L.) Raeusch. ( Poaceae ), Prunus armeniaca L. in China ( Su et al. 2023; Yang et al. 2022); Magnolia grandiflora L. ( Magnoliaceae ), Peltophorum sp. ( Fabaceae ) in Thailand ( Jayasiri et al. 2019); Mimosa diplotricha C.Wright ( Fabaceae ) in Thailand ( Hyde et al. 2024); Vigna unguiculata (L.) Walp. ( Fabaceae ) in India ( Jayawardena et al. 2022); Tephrosia cinerea (L.) Pers. ( Fabaceae ) in Thailand (this study).

Notes: Based on the BLAST results, our sequences showed high similarity to different strains of P. byssoides for the ITS, LSU, SSU, tef 1-α and rpb2 sequences. Also, based on the phylogenetic analyses, our fungal strain (MFLUCC 23- 0294) grouped well with the type (MFLUCC 20-0172) and other authentic strains (MFLUCC 19-0134, MFLUCC 22- 0132, MFLUCC 20-0137, MFLUCC 18-1548, UESTCC 18-1548, MFLUCC 18-1553, MFLUCC 17-2292, UESTCC 22-0138, NCYUCC 19-0314) of P. byssoides ( Figure 1 ). Morphologically, our collection (MFLU 23-0453) is similar to P. byssoides , which was collected from dead culms of Imperata cylindricain in China ( Su et al. 2023). We identified our fungal strain (MFLUCC 23-0453) as P. byssoides based on morpho-molecular evidence. Previously, P. byssoides have been recorded on Magnolia grandiflora , Peltophorum species, and Mimosa diplotricha in Thailand ( Jayasiri et al. 2019). Therefore, our fungal collection is the first record of P. byssoides on Tephrosia cinerea ( Fabaceae ).

Figures

FIGURE 1. Phylogram generated from maximum likelihood based on the SSU-ITS-LSU-tef1-α-rpb2 matrix. The tree was rooted with Massarina cisti (CBS 266.62) and Massarina eburnea (CBS 473.64). Bootstrap support values for ML ≥ 75% and PP ≥ 0.90 are provided at the nodes. Strains isolated in this study are in red and ex-type strains are in bold. Strains synonymized in this study are indicated in blue.

FIGURE 3. Periconia byssoides (MFLU 23-0453). a. Dead branch of Tephrosia cinerea; b, c. Colonies on the natural substrate; d–f. Conidiophores with heads of conidia; g, h. Conidiogenous cells and conidia; i–l. Conidia; m, n. Colony on PDA from above and below. Scale bars: b = 500 µm, c = 200 µm, d–f = 20 µm, g, h = 50 µm, i–l = 10 µm.