Chryseoglobus indicus, Pei & Xie & Wang & Zhang & Zhang, 2021

Pei, Shengxiang, Xie, Fuquan, Wang, Wenjing, Zhang, Shuang & Zhang, Gaiyun, 2021, Chryseoglobus indicus sp. nov., isolated from deep sea water, International Journal of Systematic and Evolutionary Microbiology (004564) 71 (1), pp. 1-5 : 4-5

DESCRIPTION OF CHRYSEOGLOBUS INDICUS SP. NOV.

Chryseoglobus indicus (in‘di.cus. L. masc. adj. indicus pertaining to India, Indian).

Following incubation for 3 days at 37 °C on modified MA, the slender rod-shaped cells (~0.2–0.3 µm×0.7–3.2 µm) are Gram-stain-positive, motile and non-spore-forming. Colonies are entire, convex, slimy, clear, yellowish in colour and approximately 1.0 mm in diameter. Good growth on modified MA and TSA, moderate growth on ISP 2 agar, and weak growth on LB and ISP 4 agar plates. Growth is observed at 10–45 °C, pH 5.0–10.0 and in the presence of 0–11% (w/v) NaCl. Optimum growth occurs at 37 °C, pH 6.0 and 0–1 % (w/v) NaCl. The activities of cellulase, amylase, xylanase and proteinase are negative. Positive for esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, cystine arylamidase, naphthol-AS-BI-phosphohydrolase and β -glucosidase. Negative for alkaline phosphatase, lipase (C 14), trypsin, α -chymotrypsin, acid phosphatase, α -galactosidase, β -galactosidase, β -glucuronidase, α -glucosidase, N - acetylβ -glucosaminidase, α -mannosidase and β -fucosidase. Analysis using the API 20 N system returns positive results for the Voges–Proskauer reaction, arabinose fermentation and gelatinase production, but negative results for citrate utilization, glucose fermentation, the production of H 2 S and indole, and catabolism of mannitol, inositol, sorbitol, sucrose, rhamnose, melibiose and amygdalin. The major cellular fatty acids are anteiso-C, anteiso-C A, iso-C 16:0 and anteiso-C 17:0. The total polar lipids are phosphatidylglycerol, diphosphatidylglycerol and three unknown glycolipids. The predominant menaquinones are MK-11 (8%), MK-12 (48%), MK-13 (31%) and MK-14 (13%). The cell-wall peptidoglycan contains lysine as a diamino acid. The DNA G+C content is 69.6mol% and the genome size is 2.59 Mb.

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The type strain, CTD02-10-2 T (=JCM 33842 T =MCCC 1A16619 T), was isolated from a deep sea water sample collected from the Indian Ocean. The NCBI GenBank accession numbers for the 16S rRNA gene sequence and the complete genome sequence of strain CTD02-10-2 T are MN463006 and CP058670 , respectively .

The 16S rRNA gene sequence and the complete genome sequence of strain CTD02-10-2 T have been deposited in the GenBank/EMBL/DDBJ databases under accession numbers MN463006 and CP058670 , respectively .