Polyplosphaeria guizhouensis X. Tang, Jayaward., R. Jeewon & J.C. Kang, 2023

Tang, Xia, Jeewon, Rajesh, Lu, Yong-Zhong, Alrefaei, Abdulwahed Fahad, Jayawardena, Ruvishika S., Xu, Rong-Ju, Ma, Jian, Chen, Xue-Mei & Kang, Ji-Chuan, 2023, Morphophylogenetic evidence reveals four new fungal species within Tetraplosphaeriaceae (Pleosporales, Ascomycota) from tropical and subtropical forest in China, MycoKeys 100, pp. 171-204 : 171

Polyplosphaeria guizhouensis X. Tang, Jayaward., R. Jeewon & J.C. Kang sp. nov.

Fig. 2

Etymology.

The specific epithet ' guizhouensis ' refers to the place where the fungus was collected, Guizhou Province, China.

Holotype.

GZAAS 23-0600.

Description.

Saprobic on unidentified decaying wood in the forest. Teleomorph not observed. Anamorph Hyphomycetous. Colonies effuse, gregarious on host substrate, brown to dark brown. Mycelium semi-immersed or immersed, pale brown, branched, septate. Conidiophores absent. Conidiogenous cells forming directly on creeping hyphae, integrated, monoblastic,determinate. Conidia 34-61 × 41-63 μm (x̅ = 51 × 51 μm, n = 20), globose to subglobose to turbinate, solitary, olivaceous-green to brown, verrucose and darker at base, with setose appendages on surface. Appendages with two forms, solitary, cylindrical, unbranched, septate, smooth, brown at base and paler towards to apex, long appendages 51-152 × 3-5 μm (x̅ = 89 × 4.0 μm, n = 20), wide at the base, 2-6-septate, arising from apical part of conidia; short appendages 13-38 × 2.5-6 μm (x̅ = 25 × 4 μm, n = 20), wide at the base, 0-3-septate, arising randomly from conidial apex.

Culture characteristics.

Conidia germinated on PDA and incubate at room temperature (25 ˚C). Colonies circular, cottony, flat, slightly grey with an undulate margin, forming three concentric zonation, margin regular, brownish grey. The reverse side is greenish grey in the centre, with a dark brown margin and pigment.

Material examined.

China, Guizhou Province, Xingyi City, Xianheping National Forest Park, on unidentified decaying wood, 25 September 2021, Xia Tang, xhp08 (GZAAS 23-0600, holotype), ex-type culture GZCC 23-0598 .

Notes.

The phylogenetic results (Fig. 1 ) showed that Polyplosphaeria guizhouensis is sister to Po. pandanicola within Polyplosphaeria with high support (ML = 100, BPP = 1). The comparison of pairwise nucleotides showed that Polyplosphaeria guizhouensis is different from Po. pandanicola in 2/801 bp (0.2%) in LSU and 11/460 (2.5%) in ITS. Thus, we describe Polyplosphaeria guizhouensis herein as a novel species in Polyplosphaeria following recommendations proposed by Jeewon and Hyde (2016) and Chethana et al. (2021).

Figures

Figure 1. Phylogenetic construction of Tetraplosphaeriaceae using RAxML-based maximum likelihood analysis of a combined LSU, ITS, SSU, and tub 2 DNA sequence dataset. Bootstrap support values for maximum likelihood (ML) equal to or greater than 70 % and Bayesian posterior probabilities (PP) equal to or greater than 0.95 PP are shown above the nodes. The tree is rooted with Amniculicola immersa (CBS 123083) and A. parva (CBS 123092). Newly generated strains are in red, and the type strains are indicated using " T " in superscript.

Figure 2. Polyplosphaeria guizhouensis (GZAAS 23 - 0600, holotype) a colonies on decaying wood b, c colonies on natural substrates d-n conidia bearing appendages o germinating conidium p colony on PDA (front at right, reverse at left). Scale bars: 50 μm (d-o).