Amphibalanus sp.

Pitriana, Pipit, Valente, Luis, von Rintelen, Thomas, Jones, Diana S., Prabowo, Romanus E. & von Rintelen, Kristina, 2020, An annotated checklist and integrative biodiversity discovery of barnacles (Crustacea, Cirripedia) from the Moluccas, East Indonesia, ZooKeys 945, pp. 17-83 : 17

Amphibalanus sp. Figure 25a-o , Table 1: species no. 91

Material examined.

Ambon Island: 1 specimen, MZB Cru Cir 135, Talake, 3°41'59.4"S, 128°10'19.2"E, coll. P. Pitriana & D. Tala, 5 Sep 2016; 1 specimen, MZB Cru Cir 136, Waitatiri, 3°37'04.0"S, 128°16'20.3"E, coll. Adin, 19 Sep 2017. Seram Island: 2 specimens, MZB Cru Cir 137, Dermaga Pelita Jaya, 3°00'13.5"S, 128°07'09.2"E, coll. P. Pitriana & D. Tala, 21 Sep 2017.

GenBank accession numbers.

COI gene (MK995349-MK995351, MK995353), 18S (MK981356-MK981358, MK981360).

Diagnosis.

Primary parietal tubes with transverse septa, exterior of shell with longitudinal striations; orifice toothed; scutum without adductor ridge; anterior margin of cirri III with conical denticles, erect hooks below posterior angles of distal articles of rami present; cirrus IV with erect hooks on posterodistal angles of articles; basidorsal of penis absent.

Description.

Shell six-plated, conical, whitish with dark purple transverse stripes (Fig. 25a, b ); orifice slightly toothed; scutum trigonal, occluding margin toothed; tergum with closed spur furrow (Fig. 25c, d ); mandible with five teeth (Fig. 25n ); maxilla bilobed with dense setae only on lower margin (Fig. 25l ). Basal length 7.4-12.2 mm, basal width 6.3-11.8 mm, height 5.5-9.4 mm; orifice length 3.6-8.3 mm; orifice width 2.6-5.9 mm (measurements for four specimens are presented in Suppl. material 1: Table S24).

Distribution.

In this study, Amphibalanus sp. was found on Ambon (at Talake and Waitatiri) and Seram islands (at Dermaga Pelita Jaya) (a map with the occurrence of Amphibalanus sp. in the Moluccas is shown in Suppl. material 1: Fig. S9).

Remarks.

In the molecular phylogeny, Amphibalanus sp. forms a well-supported clade in both, the COI and the 18S tree (Figs 28 , 29 ). This species also has different maxilla than those of A. amphitrite , A. reticulatus and A. variegatus , which have setae on its upper and lower margins.

Figures

Figure 25. Amphibalanus sp. (MZB Cru Cir 135) a upper view b side view c external view of scutum and tergum d internal view of scutum and tergum e cirrus I f cirrus II g cirrus III h cirrus IV i cirrus V j cirrus VI k penis l maxilla m maxillule n mandible o mandibular palp. Scale bars: 3 mm (a, b); 1 mm (c-g); 2 mm (h-k); 0.5 mm (l-o).

Figure 28. Bayesian phylogeny of 18 S gene sequences. High Bayesian posterior probabilities (≥ 0.85) are indicated by an asterisk at the respective node. Families with relevance for this study are highlighted by coloured rectangles. Sample labels in red indicate sequences newly generated for this study. Species names in bold indicate potential new species.

Figure 29. Bayesian phylogeny of COI gene sequences. High Bayesian posterior probabilities (≥ 0.85) are indicated by an asterisk at the respective node. Families with relevance for this study are highlighted by coloured rectangles. Sample labels in red indicate sequences newly generated for this study. Species names in bold indicate potential new species.