Blastobotrys malaysiensis Kurtzman, 2007

Ferreira de Sa, Antonio Sergio, Leonardo-Silva, Lucas & Xavier-Santos, Solange, 2022, Expanding the geographical distribution of Blastobotrys malaysiensis (Saccharomycetales) beyond the Asian continent - a cave fungus first reported in the Americas, Biodiversity Data Journal 10, pp. 80226-80226 : 80226

Blastobotrys malaysiensis Kurtzman, 2007

Materials

Type status: Other material. Occurrence: occurrenceDetails: Isolated from cave sediments; recordNumber: URM 8507/SXS 675; associatedSequences: MZ702867 ; occurrenceID: 0CC4B08A-70E3-5DE6-9209-1F6A530E0BBF; Taxon : order: Saccharomycetales ; scientificNameAuthorship: Blastobotrys malaysiensis Kurtzman , 2007; Location : higherGeography: South America : Brazil: Goiás: Parque Estadual de Terra Ronca ; continent: South America ; country: Brazil; countryCode: Brazil /BR; stateProvince: Goiás; municipality: Sao Domingos de Goiás; locality: Cave Lapa do Angelica ; decimalLatitude: -13.5173; decimalLongitude: 46.388077; Identification: identifiedBy: Sá-Ferreira A.S., Leonardo-Silva, L. Xavier-Santos, S.

Description

At five days of growth at 25, 28 and 30℃, in PDA medium, the colony showed opaque white colouration, with a mycelial fringe and lobed margin; when growing in MEA, at 27 ºC, yellowish colony, with cottony aerial mycelium in the centre and dense and opaque margin was observed. In both media, growth of septate hyphae and pseudohyphae was noted. In MEA medium, in samples from the margin of the colony, abundant spherical cells (2.74 - ˗4.50 µm) with multilateral budding were observed (Fig. 3 A˗ B); blastoconidia were also observed, formed from small pedicels (Fig. 3 C˗ D). In our cultures, ascospore production was not observed. At 37 ºC, this strain showed good growth on MEA and Mycosel agar, with a yellowish-white colony of dense aspect after seven days more abundant yeast cells were observed, with few pseudohyphae and setae. At 40 ºC, in the same culture media, after 10 days of incubation, the colony grew less than 0.5 cm beyond the inoculum, presenting a yellowish color, with a wrinkled aspect.

Habitat and distribution

Isolated from cave sediments environments. The current knowledge about its distribution reveals that the species is restricted to tropical environments, with only two records: Malaysia ( Kurtzman 2007) and Brazil (this study).

Note

B. Malaysiensis showed extensive growth at 37 ºC, with discrete development at 40 ºC. Besides to growing on medium supplemented with cyloheximide. We observed that temperature did not affect the fermentative capacity of B. malaysiensis , as the results were the same regardless of the temperature (27 or 30 ºC) (Table 2 ).

Figures

Figure 3. Morphology of Blastobotrys malaysiensis (URM 8507 / SXS 675). A Colony of B. malaysiensis; B Yeast cells, with multilateral budding (arrows); C Blastoconidia stained with lactophenol blue; D Blastoconidia attached to hyphae from pedicels (arrows). A-B grown on PDA (Potato Dextrose Agar) after eight days of growth at 27 ° C and C-D grown on MEA (Malt Extract Agar) at 27 ° C for five days. Scale bars: 10 mm (A), 10 µm (B, C, D).