Loxosceles misteca Gertsch, 1958

Valdez-Mondragon, Alejandro, Navarro-Rodriguez, Claudia I., Solis-Catalan, Karen P., Cortez-Roldan, Mayra R. & Juarez-Sanchez, Alma R., 2019, Under an integrative taxonomic approach: the description of a new species of the genus Loxosceles (Araneae, Sicariidae) from Mexico City, ZooKeys 892, pp. 93-133 : 93

Loxosceles misteca Gertsch, 1958 Figs 29-31 , 38-41 , 42-47 , 62-69

Type material.

MEXICO: Guerrero: male holotype (examined) (AMNH_IZC00327631) from Taxco, Municipality Taxco de Alarcón, Guerrero, Mexico, Date? 1946, Collected in the fall, Leo Isaacs leg.

Material examined.

MEXICO: Guerrero: 1 male, 1 female (CNAN-AR008985) from Cueva del Diablo, Acuitlapan (18.60106, -99.54318, 1581 m) Municipality Taxco de Alarcón, 04-VI-2010, O. Francke, D. Barrales, J. Cruz, A. Valdez Cols. 2 males (LATLAX-Ara 0158) from Cueva del Jardín Botánico, Parque Nacional Grutas de Cacahuamilpa (18.67038, -99.51134, 1145 m) Municipality Pilcaya, 15-IX-2017, A. Valdez, P. Solís, I. Navarro, J. Valerdi Cols. 2 males (LATLAX-Ara 0161) from Grutas del General Pacheco (18.66562, -99.50943, 1086 m) Municipality Pilcaya, 19-IX-2017, A.Valdez, P. Solís, I. Navarro, J. Valerdi Cols. 6 females (LATLAX-Ara 0162) from Cueva Agustín Lorenzo, Mexcaltepec (18.431,-99.55013, 922 m) Municipality Taxco de Alarcón, 20-IX-2017, A. Valdez, P. Solís, I. Navarro, J. Valerdi Cols. 3 males, 5 females (LATLAX-Ara 0526) from Jardín Botánico, Parque Nacional Grutas de Cacahuamilpa (18.67038, -99.51134, 1145 m) Municipality Pilcaya, 15-X-2019, A. Valdez, P. Solís, I. Navarro, A. Juaréz, A. Cabrera Cols. Morelos. 1 male (CNAN-Ar009069) from Lomas de Cortés, Municipality Cuernavaca, 11-II-2013, P. Bernard leg. 1 male (CNAN-Ar009070) from Tlaltenango (18.946414, -99.24392, 1660 m) Municipality Cuernavaca, III-2013. R. Rosas leg. 1 male (CNAN-Ar009071) from Boulevard Cuahutémoc #33, Lomas de Cortés (18.951125, -99.22408, 1640) Municipality Cuernavaca, 24-II-2012.

Diagnosis. Loxosceles misteca Gertsch, 1958 resembles L. tenochtitlan sp. nov. ( Figs 23-28 , 42-47 ); however, in L. misteca , the curvature of the basal-ventral part of the tibia of the male palp is more pronounced than in the new species ( Figs 23 , 25 , 42 , 44 , 48-55 , 62-65 , 76-77 ). Both species have a spatula-shaped embolus; in L. misteca , the embolus is slightly thinner than that of the new species ( Figs 23 , 25 , 42 , 44 , 48-55 , 62-65 , 76-77 ). Leg I length of males of L. misteca is longer than legs I of L. tenochtitlan sp. nov. ( Fig. 81 ). The seminal receptacles of the females of L. misteca and L. tenochtitlan sp. nov. are similar, however in L. misteca the distance between the base of the receptacles is shorter than in the new species ( Figs 56-61 , 66-69 ), also, the genitalia of L. misteca does not have small accessory lobes receptacles on each side, which are present in L. tenochtitlan sp. nov. ( Figs 56-61 , 66-69 ).

Figures

Figures 29 - 34. 29 - 31 Loxosceles misteca Gertsch. Male 29 left palp, retrolateral view, detail of tarsus, bulb and embolus 30 detail of bulb and embolus, retrolateral view 31 detail of the embolus 32 - 34 Loxosceles tenochtitlan sp. nov. Male paratype 32 right palp, retrolateral view, detail of tarsus, bulb and embolus 33 detail of bulb and embolus, retrolateral view 34 detail of the embolus. Arrows indicate the canal along the embolus.

Figures 38 - 41. Male holotype (examined) of Loxosceles misteca Gertsch, 1958 (AMNH _ IZC 00327631), from Taxco, Municipality Taxco de Alarcon, Guerrero, Mexico; Date? 1946, collected in the fall, Leo Isaacs leg. 38, 39 habitus of male holotype, dorsal and ventral views, respectively 40 carapace 41 label of the holotype. Scale bars: 1 mm (38 - 40).

Figures 42 - 47. Male holotype (examined) of Loxosceles misteca Gertsch, 1958 (AMNH _ IZC 00327631), from Taxco, Municipality Taxco de Alarcon, Guerrero, Mexico; 1946, collected in the fall, Leo Isaacs leg. 42 - 44 left palp, prolateral, dorsal and retrolateral views respectively 45 - 47 detail of the bulb and embolus, dorsal, retrolateral and apical views, respectively. Scale bars: 0.5 mm (42 - 44), 0.2 mm (45 - 47).

Figures 62 - 69. Loxosceles misteca Gertsch, 1958 62 - 65 variation of the male palps, left palps, prolateral views 62 Grutas General Carlos Pacheco, Municipality Pilcaya, Guerrero 63 Cueva del Diablo Acuitlalpan, Municipality Taxco, Guerrero 64 boulevard Cuauhtemoc 99, Colonia Lomas de Cortes, Municipality Cuernavaca, Morelos 65 Grutas de Cacahuamilpa National Park, Municipality Pilcaya, Guerrero 66 - 69 variation of the seminal receptacles of the females, dorsal views 66, 67 Agustin Lorenzo Cave, Mexcaltepec, Municipality Taxco de Alarcon, Guerrero 68, 69 Botanical Garden Cave, Grutas de Cacahuamilpa National Park, Municipality Pilcaya, Guerrero.

Figures 23 - 28. Loxosceles tenochtitlan sp. nov. Male Holotype 23 - 25 left palp, prolateral, dorsal and retrolateral views respectively 26 - 28 detail of the bulb and embolus, dorsal, retrolateral and apical views, respectively. Scale bars: 0.5 mm (23 - 25), 0.2 mm (26 - 28).

Figures 48 - 55. Loxosceles tenochtitlan sp. nov. Variation of the male palps, left palps, prolateral views 48 turiello Guerra, Street Cuitlahuac S / N, Tlalpan, Mexico City 49 Cruz Verde 132, Tlalpan, Mexico City (type locality) 50 Street Tepocatl 61, Pedregal de Santo Domingo, Coyoacan, Mexico City 51 Los Reyes Copilco, Frac. Areada Dpto. 102 - A, Coyoacan, Mexico City 52, 53 Street Reforma 5, Santiago Tlacochcalco, Municipality of Tepeyanco, Tlaxcala 54, 55 Street Juarez Norte 214, Huamantla, Municipality Huamantla, Tlaxcala, Mexico. Scale bars: 0.5 mm.

Figures 74 - 77. Geometric morphometry of the tibia shape on retrolateral view of the palps of males of L. tenochtitlan sp. nov. (N = 12) and L. misteca (N = 9) 74 CVA plot showing a significant difference (x 2 = 10.2555, df = 2, p = 0.00593003, λ = 0.5988) between both species in the tibiae shape 75 deformation grid, the vectors indicate the direction of change in the tibia with respect to the average shape of the 21 individuals analyzed of both species 76, 77 palps of the males of L. misteca and L. tenochtitlan sp. nov. respectively, retrolateral views (red arrows indicate the change in the shape of the tibiae of the species analyzed).

Figures 78 - 81. Sexual dimorphism of Loxosceles tenochtitlan sp. nov. (T test) 78 box plots showing the variation of leg length 1 between males and females (t = - 1.3106, p = 0.1981, df = 37, α = 0.05) 79, 80 box plots showing variation of carapace length (79) and width (80) between males and females (length: t = 1.498, p = 0.142, df = 38, α = 0.05; width: t = 0.6955, p = 0.4912, df = 36, α = 0.05) 81 linear morphometric variation of leg I length between males and females of L. tenochtitlan and L. misteca (T test) (males: t = 3.6174, p = 0.00331, df = 13, α = 0.05; females: t = 0.274, p = 0.787, df = 17, α = 0.05).

Figures 56 - 61. Loxosceles tenochtitlan sp. nov. Variation of the seminal receptacles of the females, dorsal views 56 Street Cruz Verde 132, Tlalpan, Mexico City (type locality) (female paratype) 57 Los Reyes Copilco, Fracc. Areada Dpto. 102 - A, Coyoacan, Mexico City 58 Street Juarez 23, San Mateo Ixtacalco, Municipality Cuautitlan Izcalli, Estado de Mexico 59 Street Reforma 5, Santiago Tlacochcalco, Municipality of Tepeyanco, Tlaxcala 60, 61 Street Juarez Norte 214, Huamantla, Municipality of Huamantla, Tlaxcala, Mexico.