Neodidymelliopsis tinkyukuku E.C. Keirnan, M.H. Laurence, R.G. Shivas & Y.P. Tan, 2021

Keirnan, Elizabeth C., Tan, Yu Pei, Laurence, Matthew H., Mertin, Allison A., Liew, Edward C. Y., Summerell, Brett A. & Shivas, Roger G., 2021, Cryptic diversity found in Didymellaceae from Australian native legumes, MycoKeys 78, pp. 1-20 : 1

Neodidymelliopsis tinkyukuku E.C. Keirnan, M.H. Laurence, R.G. Shivas & Y.P. Tan sp. nov. Fig. 3

Type.

Australia, South Australia, Clare, Hardenbergia violacea , 17 Sep. 2017, E.C. Keirnan (holotype BRIP 69592, includes culture ex-type).

Description.

Colonies on OA, 26-28 mm diam. after 7 d, dense low aerial mycelium, buff with numerous grey patches, darker with abundant pycnidia at centre; reverse buff to rosy buff with darker concentric rings towards centre; on MEA, 28-30 mm after 7 d, margin entire, dense low aerial mycelium, vinaceous buff paler at margin; reverse rosy buff to buff at margin with abundant scattered pycnidia; on PDA, 35-38 mm after 7 d, margin entire, dense low aerial mycelium, pale mouse grey lighter at margin; reverse cinnamon with concentric dark rings, darker at centre. NaOH spot test: light yellow. Conidiomata on CLA pycnidial, globose to ampulliform, 250-350 μm diam., brown becoming black, solitary, abundant in centre of colony, zonate, glabrous, non-papillate; ostiole c. 25 μm diam.; pycnidial wall composed of textura angularus, pale brown, cells 5-8 μm diam. Conidiogenous cells phialidic, cylindrical, thin-walled, hyaline. Conidia occasionally septate, 6-9 × 2-3 μm, cylindrical, hyaline, thin-walled.

Etymology.

From the language of the Indigenous Australian Kaurna people, meaning leaf disease. The Kaurna people are from the Adelaide plains region, which includes Clare, the locality where the holotype was collected.

Notes.

Neodidymelliopsis tinkyukuku (strain BRIP 69592) is sister to a clade that includes N. farokhinejadii (strain CBS 142853), N. longicolla (ex-type strain CBS 382.96) and N. ranunculi (strain CBS 286.72) (Fig. 1 ). Neodidymelliopsis conidial dimensions are distinct from N. farokhinejadii (4.6-7.5 × 2.4-3.9 μm), N. longicolla (12-15 × 4-7 μm), and N. ranunculi (3-5 × 7.5-10 μm). Neodidymelliopsis tinkyukuku can be easily distinguished from these three species by DNA sequences of the rpb2 locus.

Figures

Figure 1. Phylogenetic tree based on maximum likelihood analysis of the combined multilocus (rpb 2 and ITS) alignment. RAxML bootstrap values (bs) greater than 70 % and Bayesian posterior probabilities (pp) greater than 0.95 are given at the nodes (bs / pp). Genera are delimited in coloured boxes, with the genus name indicated to the right. Isolates identified in this study are in bold, and novel taxa are in red bold. Ex-type isolates are marked with T. The outgroup is Neoascochyta desmazieri (CBS 297.69).

Figure 3. Neodidymelliopsis tinkyukuku: a leaf lesions on Hardenbergia violacea b 12 - d old colonies top to bottom on PDA, MEA, OA (left, top to bottom) and lower surface (right) c upper surface d pycnidia on CLA e pycnidia f pycnidial wall g conidia. Scale bars: 300 µm (d, e); 10 µm (f); 7 µm (g).