Inocybe messapica Cervini, 2021

Cervini, Mario, 2021, Inocybe messapica (Inocybaceae, Agaricales, Basidiomycota), a new species in section Splendentes, from Mediterranean oak woods, Phytotaxa 480 (2), pp. 174-184 : 175-179

Inocybe messapica Cervini , sp. nov. ( Figs. 1–4 )

Mycobank: MB835459

Type:— ITALY. Apulia: Specchia, Cardigliano di Sopra ( LE), 160 m asl, 22 November 2019, on soil under Quercus ilex and Quercus coccifera, M. Cervini and G. Schirinzi (holotype AMB 12794!).

Diagnosis:— Inocybe messapica is characterized by the stout habit, the dark castaneous brown pileus, the pruinose stipe with pink shades and the bulbous base. The cortina is absent, but a whitish velipellis is present in young specimens, remaining on the pileus surface, especially at the margin. Smell is weak and spermatic. Spores are smooth, mostly amygdaliform, on average 10.6 × 5.9 µm. Metuloidal pleurocystidia, cheilocystidia and caulocystidia are present. Holotypus: Italy, Apulia, Specchia, Cardigliano di Sopra ( LE), 160 m asl., 22/11/2019, in open path under Quercus ilex and Quercus coccifera, Legit M. Cervini and G. Schirinzi. Stored in Herbarium AMB 12794. Etymology: the epithet messapica (from Messapi) refers to the name of the historical inhabitants of the Central Southern Apulia, Italy.

Description:— Pileus up to 55 mm, convex to hemispherical, not umbonate, dark castaneous brown (Mu 5YR 4/4–4/8; 7.5YR 3/4–3/6), glabrous to very minutely felty with involute felted margin in young specimens. Velipellis present, whitish, abundantly covering the pileus especially at the margin. Lamellae adnate-emarginate, close, whitish then ochre-argillaceous with whitish fimbriate margin. Stipe stout, central, fistulose, at first covered by the velipellis, beneath pruinose, pinkish (Mu 5YR 7/3–7/4; 7.5YR 3/4–3/6), up to 60 × 15 mm, until 22 mm at the non marginate bulb, slightly darkening at the base. Flesh white. Odor weak, spermatic.

Basidiospores (8.8)9.8–11.4(12.8) × (4.9)5.5–6.4(7.2) μm, Q = (1.4)1.6–2.0(2.5), on average 10.6 × 5.9 μm (n=106) smooth, asymmetrical, mainly amygdaliform, with subconical apex. Basidia 29–39 × 8–14 μm, tetrasporic, clavate. Pleurocystidia (36)44–57(65) × (12)16–20(24) µm, on average 51 × 19 µm (n=30) Q = (2.1)2.5–3.3(3.7), abundant, variable, slightly ventricose or subusiform, rarely elongated, thick-walled up to 3.1 μm especially at the apex, with abundant apical crystals; ammonia reaction positive. Cheilocystidia frequent, similar to pleurocystidia. Paracystidia 19–26 × 5–11 μm, subcylindric to clavate, abundant. Caulocystidia 38–54 × 13–19 µm, on average 46 × 16 µm (n = 15) similar in shape to hymenial cystidia, present all over the stipe surface, more concentrated in the upper half; arranged in tufts and mixed with cauloparacystidia. Clamp connections present in all tissues.

Specimen:— AMB 12794! ITALY. Apulia, Specchia, Cardigliano di Sopra ( LE), 22 November 2019, 160 m asl., N: 39,944° 27,035 E: 18,26722941°66,666. Legit M. Cervini and G. Schirinzi .

Ecology:—On open path, on calcareous soil under Quercus ilex and Quercus coccifera . Other occurring species: Inocybe oblectabilis (Britzelm.) Sacc. , Lactarius zugazae G. Moreno, Montoya, Band. -Muñoz & Heykoop, Russula messapica Sarnari.

Etymology:—The chosen epithet messapica (from Messapi) refers to the historical inhabitants of that area, corresponding to the actual Salento. Other tributes having the same specific name are Russula messapica Sarnari ex Sarnari (1990: 42) and Clavaria messapica Agnello, Kautman. & M. Carbone (2015: 197) .

Figures

FIGURE 1. Phylogram inferred from maximum likelihood analysis of ITS and LSU sequences of Inocybe. Species names are provided by GenBank/Unite accession numbers and country of collection. The type species is in bold. Bootstrap support values of the ML analysis ≥ 80% are displayed near each node.

FIGURE 2. Inocybe messapica: basidiomata in habitat (AMB 12794!, holotype). Photo M. Cervini.

FIGURE 3. Inocybe messapica: CH) Cheilocystidia PL); Pleurocystidia; SP) Basidiospores; VE) detail of velipellis (AMB 12794!, holotype). Photos by M. Cervini.

FIGURE 4. Inocybe messapica: BA) Basidia; CA) Caulocystidia; CH) Cheilocystidia; PA) Paracystidia; PL) Pleurocystidia; SP) Basidiospores (AMB 12794!, holotype). Drawings by M. Cervini.