Procamallanus spiculastriatus, Pinheiro, Raul Henrique da Silva, Melo, Francisco Tiago de Vasconcelos, Scott Monks,, Santos, Jeannie Nascimento dos & Giese, Elane Guerreiro, 2018

Pinheiro, Raul Henrique da Silva, Melo, Francisco Tiago de Vasconcelos, Scott Monks,, Santos, Jeannie Nascimento dos & Giese, Elane Guerreiro, 2018, A new species of Procamallanus Baylis, 1923 (Nematoda, Camallanidae) from Astronotusocellatus (Agassiz, 1831) (Perciformes, Cichlidae) in Brazil, ZooKeys 790, pp. 21-33 : 22-26

Procamallanus spiculastriatus sp. n. Figures 1, 2, 3

Material.

Type specimens. Holotype male (MPEG 195), allotype female (MPEG 196), and four paratypes (MPEG 197; MPEG 198; MPEG 199; MPEG 200) were deposited in the Coleção de Invertebrados of the Museu Paraense Emílio Goeldi (MPEG), Belém, Pará, Brazil.

Type host.

Astronotus ocellatus (Agassiz) ( Perciformes : Cichlidae ). Average length = 24.7 ± 2.6 cm; average weight = 331.8 ± 96.3 g.

Type locality.

Iará Lake, Jardim Botânico Bosque Rodrigues Alves (1°25'49"S, 48°27'22"W), Belém, Pará, Amazon Biome, Brazil.

Site in host.

Mid-intestine.

Host-parasite data.

Prevalence 55% (22 infected, 40 examined); Mean intensity = 14.8; Mean abundance = 8.5; Range = 1-59.

Etymology.

The species name refers to the unique morphology of the spicules, which membranous alae that are supported by rays, giving them a striated appearance.

Description.

[Based on 10 males, 11 females, 20 eggs (from allotype female), and 20 intrauterine larvae (from allotype female)] Medium-sized nematodes, red while alive and white after fixation. Cuticle with fine transverse striations. Oral opening circular, surrounded by three concentric circles with four papillae each, inner circle with six small pores at base proximal to oral opening, pair of small lateral amphids present (Figure 2a). Buccal capsule, orange-brown, barrel-shaped with a well-developed basal ring armed with four sclerotized tooth-like structures (Figs 1a, b, 2b). Inner surface of capsule smooth (Figure 1a, b) without ridges. Muscular esophagus somewhat shorter than glandular esophagus. Deirids minute, simple with rounded tip, situated between the buccal capsule and nerve ring (Figure 2c).

Males (based on holotype and 9 paratypes): body 8-11 (9) mm long; maximum width at esophageal/intestinal junction 105-147 (130). Buccal capsule including basal ring 57-74 (65) long and 32-39 (36) wide, basal ring 5-8 (6.5) long, 22-29 (26) wide. Maximum length/width ratio of buccal capsule 1:0.55. Deirids, nerve-ring and excretory pore at 91-119 (104), 156-188 (171) and 248-292 (263), respectively, from anterior extremity. Muscular portion of esophagus 316-395 (353) long and 42-53 (48) wide; glandular portion of esophagus 421-558 (470) long and 42-63 (51) wide. Muscular/glandular esophagus length ratio 1:1.3. Length of entire esophagus and buccal capsule 9-12% of body length. Posterior end of body ventrally curved, provided with wide caudal alae bearing six pairs of pedunculated papillae: three precloacal pairs and three postcloacal pairs (Figs 1e, 3a). Two pairs of adcloacal papillae. (Figs 1e, 3a). Two pairs of dorso-lateral sessile papillae, between cloaca and tip of tail present (Figure 3b). Caudal alae anteriorly interconnected, forming a pseudosucker, and not reaching tip of tail; a pair of phasmids located immediately posterior to the 6th pair of pedunculated papillae (Figs 1e, 3a). Spicules elongate and ventrally curved, with slightly sclerotized core; distal end of spicules with membranous alae supported by sclerotized rays (Figs 1 e–h, 3b, c). Spicules with terminal bifurcation, similar in form, bifurcation of right spicule always larger than that of left spicule. Spicules dissimilar in length; left spicule shorter, 229-284 (247) long and right spicule 312-355 (332) long (Figure 1 e–h). Gubernaculum absent. Length of tail 156-205 (184).

Females with larvae (based on 4 specimens): body 17-20 mm (18 mm) long; maximum width at esophageal/intestinal junction 160-173 (167). Buccal capsule including basal ring 78-83 (81) long and 47-52 (50) wide, basal ring 12-13 (12) long, 32-43 (36) wide. Maximum length/width ratio of buccal capsule 1:0.62. Deirids, nerve-ring, and excretory pore at 147-167 (156), 220-233 (225), and 330-397 (371), respectively, from the anterior extremity (Figure 1c). Muscular portion of esophagus 413-453 (433) long and 53-60 (58) wide; glandular portion of esophagus 600-693 (633) long and 67-80 (70) wide. Muscular/glandular esophagus length ratio 1:1.5. Length of entire esophagus and buccal capsule representing 6-7% of body length. Vulva situated at, 8-11 (9) mm from anterior end, at about 50% of body length; vulval lips not elevated. Muscular vagina directed posteriorly (Figure 1c); uterus filled with larvae 210-280 (245 ± 22 long) (Figure 1i) and eggs 17-29 (24) long by 14-26 (22) wide. Tail conical, 190-220 (208) long, without cuticular projections (Figs 1d, 3d).

Females with eggs (based on 7 specimens): body 11-13 mm (12 mm) long; maximum width at esophageal/intestinal junction 100-133 (124). Buccal capsule including basal ring 80-87 (82) long and 50-60 (53) wide, basal ring 10-12 (10) long, 33-38 (35) wide. Maximum length/width ratio of buccal capsule 1:0.64. Deirids, nerve-ring, and excretory pore at 127-163 (144), 183-227 (208), and 283-357 (308), respectively, from the anterior extremity. Muscular portion of esophagus 387-460 (427) long and 47-60 (52) wide; glandular portion of esophagus 447-527 (489) long and 60-67 (61) wide. Muscular/glandular esophagus length ratio 1:1.5. Length of entire esophagus and buccal capsule representing 8-9% of body length. Vulva situated at 6-7 mm (6 mm) from anterior end, at 52% of body length; vulval lips not elevated. Muscular vagina directed posteriorly; uterus filled with eggs 28-32 (29) long by 25-30 (26) wide. Tail conical, 140-180 (166) long, without cuticular projections.