Paraeutypella guizhouensis L.S. Dissan., J.C. Kang & K.D. Hyde, 2021

Dissanayake, Lakmali S., Wijayawardene, Nalin N., Dayarathne, Monika C., Samarakoon, Milan C., Dai, Dong-Qin, Hyde, Kevin D. & Kang, Ji-Chuan, 2021, Paraeutypella guizhouensis gen. et sp. nov. and Diatrypella longiasca sp. nov. (Diatrypaceae) from China, Biodiversity Data Journal 9, pp. 63864-63864 : 63864

Paraeutypella guizhouensis L.S. Dissan., J.C. Kang & K.D. Hyde sp. nov.

Materials

Type status: Holotype. Taxon: kingdom: Fungi; phylum: Ascomycota; class: Sordariomycetes; order: Xylariales; family: Diatrypaceae; genus: Paraeutypella; specificEpithet: guizhouensis; Location: country: China; stateProvince: Guizhou Province; county: Guiyang; locality: Guizhou University Garden (North) ; Identification: identifiedBy: L.S.Dissanayake; Event: habitat: Saprobic on dead twigs.; fieldNumber: CLD018; Record Level: type : Holotype; institutionID: HMAS 290654; collectionID: KUMCC 20-0016; institutionCode: Chinese Academy of Science , Kunming and Chinese Academy of Science Herbarium ; collectionCode: Kunming Institute of Botany Culture Collection; datasetName: CLD018 Type status: Other material. Record Level : type: isotype; institutionID: HKAS 290655; collectionID: KUMCC 20-0017; institutionCode: Chinese Academy of Science , Kunming and Chinese Academy of Science Herbarium; collectionCode: Kunming Institute of Botany Culture Collection

Description

Saprobic on dead twigs (Fig. 3 ). Sexual morph: Stromata immersed in bark of dead branches, erumpent, aggregated, circular to irregular, superficial, carbonaceous. Ascomata 590-600 × 470-480 μm (x̅ = 595 × 475 µm, n = 10), perithecial, with groups of 6-12 perithecia arranged in a valsoid configuration, subglobose, clustered, immersed in stromata, ostiolate. Neck 400-418 μm long (x̅ = 409 µm, n = 10), papillate, central ostiolar canal filled with periphyses, 3-4 sulcate. Peridium 22-35 μm wide, composed of two layers of textura angularis; inner layer cells light brown to hyaline, outer layers cells dark brown to black. Hamathecium hyaline. Paraphyses 1-2 μm wide (x̅ = 1.5 µm, n = 10), arising from base of perithecia, long, narrow, unbranched, septate, guttulate, narrowing and tapering towards apex. Asci 55-80 × 5-9 μm (x̅ = 67.5 × 7 μm, n = 20), 8-spored, unitunicate, thin-walled, clavate to cylindrical clavate, long pedicellate (25-30 μm), with a J- apical ring. Ascospores 7-11 × 1-3 μm (x̅ = 9 × 2 μm, n = 30), overlapping biseriate, allantoid, hyaline to light brown, smooth, aseptate, usually with 2-3 guttules. Asexual morph: Undetermined.

Culture characteristics - Colonies on PDA, reaching 21 mm diam. after 2 weeks at 20-25oC, medium dense, circular to slightly irregular, slightly raised, cottony surface; colony from above: at first white, becoming buff; from below: yellowish-white at margin, yellow to brown at centre; mycelium yellowish.

Etymology

The specific epithet Paraeutypella guizhouensis refers to the locality in which the fungus was collected.

Notes

Paraeutypella guizhouensis resembles P. vitis , which comprises stromata that are erumpent through bark, with elongated perithecial necks and allantoid, slightly to moderately curved ascospores ( Glawe and Jacobs 1987). However, P. guizhouensis differs from P. vitis in having comparatively longer ostiolar necks and longer asci (55-80 × 5-9 μm), while P. vitis has comparatively shorter ostiolar necks and shorter asci (40-46 × 6-8 μm) ( Glawe and Jacobs 1987). Paraeutypella vitis (UCD2428TX) differs phylogenetically from our new taxon in 14% (80/576) base pairs in the ITS and 10% (42/405) base pairs in β-tubulin. Thus, P. guizhouensis is introduced as a new species in Paraeutypella , based on its morphology, base pair differences and phylogenetic analyses (94% ML, Fig. 1).

Figures

Figure 3. Paraeutypella guizhouensis (HMAS 290654, holotype) a-c. stromata on substrate; d. cross section of a stromata; e. vertical section through an ascostroma showing ostioles and perithecia; f. ostiolar canal; g. peridium; h. paraphyses; i-l. asci; m-o. ascospores; p. germinating ascospore; q, r. cultures on PDA from above and below after 6 weeks. Scale bars: 500 µm (b-d), 200 µm (e), 100 µm (f), 20 µm (g-l), 5 µm (m-p).