Phyllosticta anhuiensis Ning Jiang & C.B. Wang, 2023

Wang, Cheng-Bin, Yang, Jing, Li, Yong, Xue, Han, Piao, Chun-Gen & Jiang, Ning, 2023, Multi-gene phylogeny and morphology of two new Phyllosticta (Phyllostictaceae, Botryosphaeriales) species from China, MycoKeys 95, pp. 189-207 : 189

Phyllosticta anhuiensis Ning Jiang & C.B. Wang sp. nov.

Fig. 2

Etymology.

Referring to the Anhui Province, where the species was first collected.

Description.

Sexual morph: Unknown. Asexual morph: Conidiomata pycnidial, aggregated, black, erumpent, globose to pyriform, exuding gray to pale yellow conidial masses, 100-400 µm diam. Conidiophores subcylindrical to ampulliform, reduced to conidiogenous cells. Conidiogenous cells phialidic, hyaline, thin-walled, smooth, subcylindrical to ampulliform, 10-16 × 2.5-4.5 μm. Conidia 8.5-12 × 5.5-9 μm, (mean ± SD = 10 ± 1 × 7.2 ± 0.7 μm), solitary, hyaline, aseptate, thin and smooth-walled, coarsely guttulate, globose or ellipsoid to obvoid, enclosed in a thin persistent sheath, 1-1.5 μm thick, and bearing an apical mucoid appendage 4-6 × 1-2 μm, flexible, unbranched, tapering towards an acutely rounded tip.

Culture characters.

Colonies on PDA flat, with irregular edge, slow growing, grayish-green to green, reaching a 90 mm diameter after two weeks. Colonies on MEA flat, undulate at the edge, slow growing, gray-white to gray, reaching a 70-80 mm diameter after two weeks. Colonies on SNA flat, slow growing, celandine green, reaching a 60-70 mm diameter after two weeks.

Specimens examined.

China, Anhui Province, Hefei City, leaf spots of Quercus aliena , Yong Li & Dan-ran Bian, 10 August 2019 (holotype CAF800072; ex-type culture: CFCC 54840). Ibid. (cultures: CFCC 55887 and CFCC 58849) .

Notes.

In the phylogeny analyses, P. anhuiensis groups sister to P. kerriae (MAFF 240047). P. kerriae was associated with Kerria japonica in Japan ( Motohashi et al. 2008). Comparison of DNA sequences of P. anhuiensis with P. kerriae (MAFF 240047), there is 99.4% (447/480 identities; 0/480 gaps) sequence similarity in ITS, 99.8% (554/555 identities, 0/480 gaps) in LSU, 98.6% (215/218 identities, 0/218 gaps) in tef1, and 97.7% (212/217 identities, 0/217 gaps) in act. Morphologically, P. anhuiensis can be distinguished from P. kerriae in having shorter appendage (4-6 µm in P. anhuiensis vs. 5-12.5 µm in P. kerriae ) ( Motohashi et al. 2008). Therefore, this species was regarded as a new species based on morphology and multi-locus phylogeny.

Figures

Figure 2. Morphology of Phyllosticta anhuiensis (CFCC 54840) A diseased leaf of Quercus aliena B conidiomata C conidiogenous cells D, E conidia F-H colonies on PDA, MEA and SNA after two weeks at 25 ° C. Scale bars: 500 μm (B); 10 μm (C-E).