Neofusicoccum occulatum Sakalidis & T. Burgess

Ma, Xiao-Ya, Hyde, Kevin D., Phillips, Alan J. L., Kang, Ji-Chuan, Chomnunti, Putarak & Doilom, Mingkwan, 2021, Three new host records of endophytic Neofusicoccum species reported from Dendrobium orchids, Phytotaxa 494 (2), pp. 193-207 : 198

Neofusicoccum occulatum Sakalidis & T. Burgess ( FIGURE. 2 )

Description:— Colony on PDA superficial, grey, fluffy, irregular edge; reverse dark brown to black, with concentric rings, growth rate: 2.5 mm /day. Colony on WA was similar to that on PDA. Vegetative mycelia 2–4.5 μm diam, hyaline to brown, septate, smooth. Conidiomata found under mycelia, black, aggregated, irregular. Conidia (15–) 17.5 – 22 (–23) × (5–) 5.5 – 6 (–6.5) μm (x = 20 × 6 μm, L:W = 3.3), fusiform to ellipsoidal with obtuse apex, hyaline, smooth, thin-walled, septate, with minute guttules.

Material examined:— CHINA, Guizhou Province, Guiyang City, Animal Husbandry and Veterinary institute, endophytic in the stem of Dendrobium chrysanthum , 11 April 2016, S . X. Zhou & X. Y . Ma, living culture, MFLUCC 20-0234 .

Notes:— Blast results for MFLUCC 20-0234 hit Neofusicoccum ribis ( ITS, 100% similarity), N. parvum ( TUB 2, 100% similarity) and Neofusicoccum sp. (EF-1α, 99% similarity). All sequences of MFLUCC 20-0234 are identical with those of CBS 128008 ( N. occulatum ), which is the type. Morphological characteristics of MFLUCC 20-0234 are the same as the type strain CBS 128008 and those of pathogenic strains isolated from Eucalyptus spp. by Sakalidis et al (2011). Therefore, we identified our isolate as Neofusicoccum occulatum .

Figures

FIGURE 2. Neofusicoccum occulatum on PDA (MFLUCC 20-0234) A–B. Colony on PDA (A: surface, B: reverse). C. Conidiomata masses. D–G. Conidia. Scale bars: C=500 μm, D–G=10 μm.