Diaporthe apiculatum Y.H. Gao & L. Cai, in Gao, Liu & Cai, Syst. Biodiv. 14: 106. 2016.

Yang, Qin, Jiang, Ning & Tian, Cheng-Ming, 2021, New species and records of Diaporthe from Jiangxi Province, China, MycoKeys 77, pp. 41-64 : 41

Diaporthe apiculatum Y.H. Gao & L. Cai, in Gao, Liu & Cai, Syst. Biodiv. 14: 106. 2016. Figure 2

Description.

Conidiomata pycnidial, discoid, immersed in bark, scattered, slightly erumpent through bark surface, with a solitary undivided locule. Ectostromatic disc yellowish to grey, one ostiole per disc, (300-)305-357(-368) μm diam. Ostiole medium black, up to level of disc. Locule undivided, (338-)357-450(-464) μm diam. Conidiophores reduced to conidiogenous cells. Conidiogenous cells cylindrical, hyaline, densely aggregated, phiailidic, unbranched, straight or slightly curved. Beta conidia hyaline, aseptate, filiform, hamate, eguttulate, base subtruncate, tapering towards one apex, (26.5-)30-39.5(-43) × 1.5-2 µm. Alpha conidia not observed.

Culture characters.

Colony originally flat with white fluffy aerial mycelium, becoming yellowish to pale green mycelium with age, marginal area irregular, conidiomata absent.

Specimens examined.

China. Jiangxi Province: Ganzhou City, Fengshan Forest Park, on branches of Rhus chinensis , 25°45'12"N, 115°00'41"E, 23 Jul 2018, Q. Yang, Y. Liu, Y.M. Liang & C.M. Tian (BJFC-S1680; living culture: CFCC 53068, CFCC 53069 and CFCC 53070).

Notes.

Diaporthe apiculatum was originally described as an endophyte from healthy leaves of Camellia sinensis in Jiangxi Province, China ( Gao et al. 2015). In the present study, three isolates (CFCC 53068, CFCC 53069 and CFCC 53070) from symptomatic branches of Rhus chinensis were found congruent with D. apiculatum , based on DNA sequence and morphological data (Fig. 1 ). The clade was, therefore, confirmed to be D. apiculatum and was found to be both an endophyte and a pathogen.

Figures

Figure 1. Phylogram of Diaporthe from a Maximum Likelihood analysis based on combined ITS, cal, his 3, tef 1 and tub 2. Values above the branches indicate Maximum Likelihood bootstrap (left, ML BP ≥ 50 %) and Bayesian probabilities (right, BI PP ≥ 0.90). The tree is rooted with Diaporthella corylina. Strains in current study are in blue font and the ex-type cultures are in bold font.

Figure 2. Diaporthe apiculatum on Rhus chinensis (BJFC-S 1680) a, b habit of conidiomata in wood c transverse section of conidiomata d longitudinal section through conidiomata e conidiogenous cells attached with beta conidia f the colony on PDA. Scale bars: 200 μm (b-d); 10 μm (e).