Teunia mussaendrae Y. Z. Qiao & F. L. Hui, 2024

Guo, Qi-Chao, Liu, Shan, Qiao, Ya-Zhuo & Hui, Feng-Li, 2024, Three new species of Teunia (Cryptococcaceae, Tremellales) identified through phenotypic and phylogenetic analyses, MycoKeys 105, pp. 139-153 : 139-153

Teunia mussaendrae Y. Z. Qiao & F. L. Hui sp. nov.

Fig. 3 C

Etymology.

Referring to Mussaenda , the genus of the plant where the type strain was isolated.

Typus.

China. Guizhou Prov. Pingtang Co., Sifangjing Vil .; in the phylloplane of Mussaenda pubescens ; Feb 2023; D. Lu; NYNU 23232 (holotype CICC 33594 T, culture ex-type PYCC 9974 ; GenBank Nos OQ 851888 , OQ 851887 , PP 236729 ) .

Description.

On YM agar after seven days at 20 ° C, the streak culture was yellowish-cream, mucoid and smooth, entire margin. After seven days in YM broth at 20 ° C, cells isolated were globose to ovoid, 3.5–5 × 4.5–6 μm, budding polar. After one month at 20 ° C, a ring and sediment was present. In Dalmau plate culture on CMA, no hyphae or pseudohyphae were formed. Sexual structures were not observed in any of the strains or when strains were paired on PDA, CMA or V 8 agar. Glucose fermentation was absent. Glucose, inulin, sucrose, galactose, lactose, trehalose, maltose, melezitose, cellobiose, salicin, L-sorbose, L-rhamnose, D-xylose, L-arabinose, D-arabinose, 5 - keto-D-gluconate, D-ribose, ethanol (weak), glycerol (weak), ribitol, galactitol, D-mannitol, D-glucitol, myo-inositol, DL-lactate, succinate, D-gluconate, D-glucosamine (weak), 2 - keto-D-gluconate, D-glucuronate, and glucono- 1, 5 - lactone were assimilated as carbon sources; raffinose, melibiose, methanol, erythritol, and N-acetyl-D-glucosamine were not assimilated. Ethylamine (delayed), L-lysine, and cadaverine (delayed) were assimilated as nitrogen sources; nitrate and nitrite were not assimilated. Maximum growth temperature was 25 ° C. Starch-like substances were not produced. Urease activity and Diazonium Blue B reaction were positive.

Additional strain examined.

China. Guizhou Prov. Pingtang Co., Sifangjing Vil.; in the phylloplane of Viburnum sp. ; Feb 2023; D. Lu; NYNU 23257 (GenBank Nos: PP 239074, PP 239072, PP 236730).

Note.

Based on the D 1 / D 2 sequences, T. mussaendrae sp. nov. was most closely related to T. globosa . It can be differentiated from T. globosa by the ability to assimilate L-sorbose, L-arabinose, D-arabinose, ribitol, galactitol, D-glucitol, and D-gluconate. Additionally, T. mussaendrae sp. nov. can grow in vitamin-free medium at 25 ° C, while T. globosa cannot.

Figures

Figure 3. Vegetative cells of A T. acericola sp. nov. NYNU 2111141 T B T. qingyuanensis sp. nov. NYNU 22475 T and C T. mussaendrae sp. nov. NYNU 23232 T, following 7 days of growth in YM broth at 20 ° C. Scale bars: 10 μm.