Lasioseius piracicabensis Moraes & Pérez-Madruga, 2015

De Moraes, Gilberto J., Abo-Shnaf, Reham I. A., Pérez-Madruga, Yanebis, Sánchez, Leocadia, Karmakar, Krishna & Ho, Chyi-Chen, 2015, The Lasioseius phytoseioides species group (Acari: Blattisociidae): new characterisation, description of a new species, complementary notes on seven described species and a taxonomic key for the group, Zootaxa 3980 (1), pp. 1-41 : 22-25

Lasioseius piracicabensis Moraes & Pérez-Madruga , n. sp.

( Figs 47–63 )

Diagnosis. Dorsal shield mostly reticulate, with 23 pairs of setae of rather distinct lengths; setae j3, j5, j6, J2, J4, J5, z2, s4, s6, r2, r5 and R1–R5 the shortest, smooth (except for J5, serrate); j2, j4, Z3–Z5, s5 and r3 relatively long, serrate; z5 and S2–S5 intermediate in length, serrate. With a pair of lightly sclerotised presternal plates. With six pairs of setae on unsclerotised cuticle lateral to dorsal shield. Spermathecal apparatus lightly sclerotised, with calyx apparently tubular; minor duct apparently relatively wide, enlarging further into an ellipsoidal section before connecting to atrium.

Adult female. Dorsum of idiosoma ( Fig. 47 ): dorsal shield 494 (475–518) long and 305 (288–323) wide at level of j6. Podonotal region of dorsal shield sparsely reticulate, with 13 pairs of setae (j2–j6, z2, z4, z5, s4–s6, r2, r3), three pairs of distinct lyrifissures and six pairs of pores. Opisthonotal region completely reticulate, with ten pairs of setae (J2, J4, J5, Z3–Z5, S2–S5), 12 pairs of distinct lyrifissures and three pairs of pores. Setae j3, j5, j6, J2, J4, J5, z2, s4, s6, r2, r5 and R1–R5 the shortest, smooth (except J5, serrate); j2, j4, Z3–Z5, s5 and r3 relatively long, serrate; z5 and S2–S5 intermediate in length, serrate. Lateral cuticle with six pairs of setae (r5, R1–R5) and a pair of lyrifissures between R3 and R4; setae short and aciculate. Setal lengths: j2 55 (51–60), j3 9 (8–10), j4 53 (49–56), j5 9 (8–11), j6 13 (12–16), J2 13 (11–15), J4 16 (15–20), J5 16 (14–18), z2 10 (9–11), z4 57 (53–60), z5 40 (34– 44), Z3 91 (88–93), Z4 84 (80–86), Z5 82 (74–85), s4 13 (12–15), s5 69 (65–74), s6 20 (19–23), S2 26 (24–31), S3 23 (22–27), S4 27 (24–30), S5 42 (34–52), r2 8 (6–9), r3 64 (61–67), r5 13 (12–15), R1 11 (10–13), R2 12 (11–13), R3 11 (10–11), R4 12 (10–14), R5 12 (10–15).

Venter of idiosoma ( Figs 48–49 ): all setae aciculate and smooth, except for JV5, thicker and distally serrate. Ventral shields reticulate. Base of tritosternum 19 (13–21) long, 8 (8–9) wide at median level; often with other transverse lines at 7 (6–8) and 34 (34–35) from base; laciniae 85 (69–110) long, divided for about 45% of their total length. Presternal region transversely striate, with a pair of lightly sclerotised presternal plates. Sternal shield fused with anterior portion of endopodal shield, reticulate, 92 (83–100) long, 102 (85–108) wide at level of st2, bearing three pairs of setae (st1–st3) and two pairs of lyrifissures. Metasternal plate round to pear shaped, bearing the fourth pair of sternal seta (st4) and a pair of lyrifissures (iv4). Genital shield 137 (133–140) long, including the hyaline flap; posterior margin truncate, 95 (88–100) wide at posterior corners; bearing genital setae (st5); distance between genital setae 74 (70–77); paragenital lyrifissures (iv5) on unsclerotised cuticle, posterolateral to st5. Ventrianal shield 175 (160–183) long and 238 (230–243) wide at widest level, bearing six pairs of opisthogastric setae (JV1–JV4, ZV2, ZV3) in addition to the circum-anal setae, 1–2 pairs of lyrifissures (one or both lyrifissures close to the anterior margin inserted on or off the shield) and a pair of pores on the margin of the shield, posterolateral to para-anal seta; anal opening small, 29 (27–32) long including frame. Opisthogastric cuticle with a continuous sclerotised line between genital and ventrianal shields and two pairs of metapodal plates, the anterior smaller than the posterior. Unsclerotised cuticle around ventrianal shield with 3–4 pairs of lyrifissures and with JV5. Posterior portion of endopodal shield shaped as a tri-radiate plate between coxae III–IV. Exopodal plate distinct from anterior margin of coxa II to posterior margin of coxa IV. Length of ventral setae: st1 34 (31–36), st2 29 (27–31), st3 27 (25–31), st4 25 (24–26), st5 22 (20–24), JV1 17 (15–18), JV2 24 (23–26), JV3 35 (33–36), JV4 32 (31–34), JV5 59 (55–61), ZV2 24 (21–26), ZV3 18(14–20), para-anal 20 (19–21) and post-anal 36 (33–39).

Peritrematic plate and peritreme ( Figs 50 ): peritrematic plate fused with dorsal shield at level of j2 and broadly fused with exopodal plate beside coxa IV; with a lyrifissure and a pore in region between coxae II–III (ip1, gp2), two lyrifissures and a pore behind stigma (ip2, ip3, gp2), and with a pore near posterior end [SJ, inguinal solenostome of Athias-Henriot, 1969, gv2 of Lindquist & Moraza, 2009]; peritreme extending forward to region between j2 setae.

Spermatheca ( Fig. 51 ): spermathecal apparatus lightly sclerotised, with calyx apparently tubular; minor duct apparently relatively wide, enlarging further into an ellipsoidal section before connecting to barely distinguishable atrium.

Gnathosoma ( Figs 52–54 ): general shape of chelicera as for the new characterisation of the phytoseioides group; fixed digit 47 (45–50) long, bearing 14 teeth in addition to apical tooth; hyaline rim with about 12 teeth; movable digit 45 (44–46) long, bearing three teeth in addition to the apical tooth. Anteromedian region of epistome convex and denticulate. Deutosternum with seven transverse lines of denticles; first (distal) to fifth with 2–4 denticles each; sixth and seventh with respectively 12–15 and 11–15 denticles. Lengths of setae on hypostome: h1 31 (25–35), h2 26 (24–32), h3 28(26–30) and sc 35 (31–38).

Legs ( Fig. 55 ): leg pretarsi I–IV each with a pair of claws and pulvillus with three rounded lobules. Leg lengths: I: 622 (605–650), II: 470 (453–493), III: 470 (448–498) and IV: 654 (635–695). Leg IV with three macrosetae: on genu (ad2) 58 (55–65), distally serrate, on basitarsus (pd3) 64 (60–67), distally serrate, on telotarsus (pd2) 105 (100–110), smooth. Leg chaetotaxy (trochanter to tibia): I: 1, 0/1,1/2,1; 2, 3/2, 2/1, 2; 2, 3/1, 3/ 2, 2; 2, 3/2, 3/1, 2; II: 1, 0/1, 0/2, 1; 2, 3/1, 2/2, 1; 2, 3/1, 2/1, 2; 1, 2/1, 3/1, 2; III: 1, 1/1, 1/1, 0; 1, 2/1, 1/0, 1; 2, 2/ 1, 2/1, 1; 2, 1/1, 2/1, 1; IV: 1, 1/1, 0/1, 1; 1, 2/1, 1/0, 1; 2, 2/1, 3/0, 1; 2, 1/1, 3/1, 2.

Adult male. Dorsum of idiosoma ( Fig. 56 ): dorsal shield 357 long and 225 wide at level of j6. Podonotal and opisthonotal regions of the dorsal shield with reticulation and numbers of setae as in adult female; with respectively three and six pairs of lyrifissures and one and three pairs of pores. Relative lengths and shape of setae as in adult female. Lateral cuticle with two pairs of setae (r5 and R1); both setae short and aciculate. Setal lengths: j2 44, j3 7, j4 46, j5 (broken), j6 11, J2 11, J4 (broken), J5 9, z2 5, z4 43, z5 15, Z3 74, Z4 65, Z5 66, s4 13, s5 51, s6 12, S2 17, S3 16, S4 15, S5 23, r2 10, r3 56, r5 10, R1 10.

Venter of idiosoma ( Figs 57–58 ): shape of setae as in adult female, except for JV5, smooth. Tritosternum with transverse lines at 7, 13 and 20 from base, 6 wide at median level; laciniae 32 long, divided for about 43% of their total length. Presternal region transversely striate, with a pair of lightly sclerotised presternal plates. Sternogenital shield reticulate, fused with whole endopodal shield, 160 long, 90 wide at level of st2, bearing five pairs of setae (st1–st5) and three pairs of lyrifissures. Ventrianal shield subtriangular, 144 long and 108 wide at anterior corners, reticulate, bearing five pairs of opisthogastric setae (JV1–JV3, JV5 and ZV3) in addition to the circum-anal setae; anal opening small, 23 long including frame. Unsclerotised cuticle around ventrianal shield without opisthogastric setae. Exopodal plate distinct from anterior margin of coxa II to posterior margin of coxa IV. Length of ventral setae: st1 28, st2 27, st3 24, st4 19, st5 20, JV1 18, JV2 21, JV3 28, JV5 34, ZV3 18, para-anal 18 and post-anal 25.

Peritrematic plate and peritreme ( Fig. 59 ): peritrematic plate fused with dorsal shield at level of r3 and broadly fused with exopodal plate beside coxa IV; with a lateral expansion in the region between coxae II–III containing a lyrifissure and a pore (ip1, gp2), with two lyrifissures and a pore behind stigma (ip2, ip3, gp2), and with a pore near posterior end [SJ, inguinal solenostome of Athias-Henriot, 1969, gv2 of Lindquist & Moraza, 2009]; peritreme extending forward to region between j2 setae.

Gnathosoma ( Figs 60–62 ): fixed digit about 30 long, bearing 12 teeth in addition to apical tooth, and a reduced antiaxial projection posteriad of setiform pilus dentilis; movable digit about 38 long, bearing a single tooth in addition to the apical tooth and a slightly twisted spermadactyl, about 32 long. Anteromedian region of epistome as in adult female. Deutosternum with seven transverse lines of denticles; first (distal) to fifth with four tiny denticles each; sixth and seventh with respectively about 26 and 13 larger denticles; sixth line M-shaped, other lines about straight. Lengths of setae on hypostome: h1 28, h2 26, h3 27 and sc 32.

Legs ( Fig. 63 ): legs similar to female. Leg lengths: I: 488, II: 300, III: 300 and IV: 500. Leg IV with three macrosetae: on genu (ad2) 50, distally serrate, on basitarsus (pd3) 60, smooth, on telotarsus (pd2) 100, smooth. Leg chaetotaxy as in adult female.

Type specimens. Holotype female and six paratype females from rice plants, collected by Y. Pérez-Madruga, at Escola Superior de Agricultura “Luiz de Queiroz” ( ESALQ) campus, Universidade de São Paulo ( USP), Piracicaba, São Paulo state, Brazil, July 12, 2012. Two paratype females and one paratype male from Ageratum conizoides L., collected by J.A. Freitas, at Cambuí, Minas Gerais state, Brazil, January 24, 2013. All types deposited in the mite collection of Departamento de Entomologia e Acarologia, ESALQ-USP.

Etymology. The species name piracicabensis refers to “from Piracicaba”, locality where the holotype was collected.

Remarks. L. piracicabensis n. sp. is unique among the species in the phytoseioides group by not having seta Z1 and by having very short j3, z2 and s 4 in contrast with the nearest setae.

Figures

FIGURES 47 – 55. Lasioseius piracicabensis Moraes & Pérez-Madruga n. sp. Holotype female: 47. Dorsal shield; 48. Tritosternum; 49. Ventral shields; 50. Peritrematic plate; 51. Spermatheca; 52. Chelicera; 53. Epistome; 54. Hypostome; 55. Genu, tibia and tarsus of leg IV.

FIGURES 56 – 63. Lasioseius piracicabensis n. sp. Paratype male: 56. Dorsal shield; 57. Tritosternum; 58. Ventral shields; 59. Peritrematic plate; 60. Spermadactyl; 61. Epistome; 62. Hypostome; 63. Leg IV.