Distoseptispora lanceolatispora X.M. Chen & Y.Z. Lu, 2024

Chen, Xue-Mei, Tang, Xia, Ma, Jian, Liu, Ning-Guo, Tibpromma, Saowaluck, Karunarathna, Samantha C., Xiao, Yuan-Pin & Lu, Yong-Zhong, 2024, Identification of two new species and a new host record of Distoseptispora (Distoseptisporaceae, Distoseptisporales, Sordariomycetes) from terrestrial and freshwater habitats in Southern China, MycoKeys 102, pp. 83-105 : 83

Distoseptispora lanceolatispora X.M. Chen & Y.Z. Lu sp. nov.

Fig. 3

Etymology.

Referring to the lanceolate conidia.

Holotype.

GZAAS 22-2045.

Description.

Saprobic on submerged decaying wood in freshwater habitat. Sexual morph: Undetermined. Asexual morph: Colonies on substrate effuse, gregarious, hairy, pale brown to brown. Mycelium mostly immersed, composed of septate, yellow-brown to brown, smooth hyphae. Conidiophores 120-190 × 4-8 µm (x- = 155 × 6.5 µm, n = 20), macronematous, mononematous, erect, solitary, straight or slightly flexuous, grayish brown to dark brown, slightly tapering towards the apex, cylindrical, 7-8-septate, unbranched, thick-walled, smooth-walled. Conidiogenous cells 15-27 × 3-5.5 µm (x- = 20.5 × 4.5 µm, n = 20), monoblastic, integrated, terminal, cylindrical, slightly tapering towards the apex, pale brown, percurrent. Conidia 31-90 × 9.5-15 µm (x- = 58.5 × 13 µm, n = 20), acrogenous, solitary, fusiform or lanceolate, rostrate, truncate at the base, straight or slightly curved, 5-13-distoseptate, slightly constricted at septa, olivaceous to olivaceous brown, slightly paler at the apex, verrucous, with or without apical, hyalina appendages.

Culture characteristics.

Colonies grown on PDA circular, dense, flat, dry, gray to dark gray, radially striated, and a ring in the middle of the colonies with an entire edge from the front view, dark brown to black with a circular, gray edge from reverse view, not pigmented.

Material examined.

China, Hainan Province, on submerged decaying wood in a freshwater stream, 23 October 2021, Jian Ma, J 13 (GZAAS 22-2045, holotype), ex-type living culture, GZCC 22-2045 .

Notes.

Distoseptispora lanceolatispora is morphologically similar to D. leonensis (M.B. Ellis) R. Zhu & H. Zhang. However, compared to D. lanceolatispora , D. leonensis has longer conidiophores (120-190 µm vs. 110-130 µm), longer conidiogenous cells (15-27 µm vs. 5-15 µm), and 5-13-distoseptate, fusiform or lanceolate conidia ( Zhang et al. 2022). In the phylogenetic analyses (Fig. 1 ), D. lanceolatispora forms a unique clade adjacent to D. neorostrata D.F. Bao, Z.L. Luo & H.Y. Su with 100% ML, 1 PP, and 98% MP support. Based on a pairwise nucleotide comparison of ITS and LSU sequences, D. lanceolatispora deviates from D. neorostrata by 39/529 bp (6.8%) for ITS and 14/850 bp (1.6%) for LSU, and there is no data available for tef 1-α and rpb 2 for D. neorostrata (MFLUCC 18-0376) in GenBank. Hence, we introduce the new species, D. lanceolatispora , based on the criteria established by Jeewon and Hyde (2016) and Chethana et al. (2021).

Figures

Figure 1. Phylogenetic tree generated from ML analysis based on a combination of LSU, ITS, tef 1 - a, and rpb 2 sequence data. Bootstrap support values of ML and MP equal to or greater than 75 %, and PP value equal to or greater than 0.95 are given near the nodes as ML / PP / MP. The tree is rooted with Aquapteridospora aquatica (MFLUCC 17 - 2371). Ex-type strains are indicated by the superscript T. The new collections are in bold red text.

Figure 3. Distoseptispora lanceolatispora (GZAAS 22 - 2045, holotype) a, b colonies on substrate c-e conidiophores and conidia f, g conidiogenous cells bearing conidia h-k conidia l germinated conidium m, n colony on PDA (m from front n from reverse). Scale bars: 50 μm (c-g); 30 μm (h-l).