Linta, WESTERGREN & SIDDALL, 2004

WESTERGREN, STEPHANIE & SIDDALL, MARK E., 2004, Two New Species of Salifid Leeches (Arhynchobdellida: Erpobdelliformes: Salifidae) from South Africa and Madagascar, American Museum Novitates 3456 (1), pp. 1-7 : 3-4

Linta be, new species

Figures 1–8

DESCRIPTION: One holotype, AMNH Annelida 5258, fixed in 10% buffered formalin and stored in 70% ethanol. Forty­two paratypes, AMNH Annelida 5259, fixed in 10% buffered formalin and stored in 70% ethanol. Fifty­eight paratypes, AMNH Annelida 5260, fixed and stored in 95% ethanol. With characters of the genus. Adults up to approximately 35 mm in length. Body cylindrical, vermiform (fig. 1). Dorsum reddish brown to dark gray in life; pale gray and random mottling postfixation. Annulation formula of midbody somites: b1, b2, a2, b5, c11, c12 (figs. 3, 5). Anus dorsal on XXVII, four postanal annuli anterior to caudal sucker. Caudal sucker ventrally directed in relaxed specimens. Eyes: faded considerably when fixed, especially in ethanol; first and second pair dorsal, on annulus, separated by two annuli; third pair lateral, on furrow, separated from second by two and one­half annuli; fourth lateral, on annulus, separated from third by two and one­half annuli; fifth lateral, at furrow, separated from fourth by four and onehalf annuli (figs. 2, 3). Genital pores separated by six annuli (fig. 5). Male gonopore at XII b1/b2. Female gonopore at XIII b1/ b2. Pharyngeal stylet pairs small, digitiform, often stylet pore only visible (fig. 4). Ovaries saccate from XII/XIII to XIV/XV (fig. 6). Male median reproductive apparatus large with pair of atrial horns directed posteriolaterally (fig. 7); ‘‘prostate’’ glandular tissue situated anterior at junction of atria (figs. 6, 7). Sperm ducts thick, in loose coil from atria to XV (fig. 7). Testisacs paired, arranged interganglionically in paramedial rows of six between XV and XVI, XVI and XVII, XVII and XVIII, XVIII and XIX, and XIX and XX, for a total of 30 pairs (fig. 8).

TYPE LOCALITY: Esana , 5 km west of Tolagnaro, Madagascar , 25 ° 1 ' 18 ̎ S, 4 ° 39 ' 26 ̎ E.

ETYMOLOGY: The species is named for the Malagasy word ‘‘be’’ (pron. bay) meaning ‘‘very many’’ or ‘‘very much’’ or ‘‘great’’ in light of this species being very much a leech and being found in large numbers notwithstanding that it had gone undiscovered for so long.

REMARKS: The new genus Linta possesses several characters of the family Salifidae . In particular the presence of pharyngeal stylets is diagnostic for the family. El­Shimy (1996) proposed a new family, Barbronidae, to accommodate Barbronia and Vivabdella , suggesting that the accessory copulatory pores merit a family separate from Salifidae . This change would present the problem of finding a new synapomorphy for Salifidae other than pharyngeal stylets, which are possessed by all of the current salifid genera. The midbody annulation formula for Linta , consisting of six annuli per somite, is similar to species in the genus Barbronia (e.g., El­Shimy, 1996; Blanchard, 1897b). However, Linta also is similar to Salifa in that it lacks accessory copulatory pores and in the position of gonopores at XII b1/b2 and XIII b1/b2 (e.g., Moore, 1939; Blanchard, 1897a). Linta is distinct from all other salifid genera in the size and shape of its atrium, the position of atrial horns, and the presence of glandular tissue on the atrium. Where atrial horns are present in other salifid species (e.g., Nesemann, 1995) they are directed anteriolaterally, as opposed to their posteriolateral position in Linta . In contrast to any other erpobdelliform leech, Linta also has five pairs of eyespots (not ocelli) arranged in a parabolic arc, in a characteristic manner that is otherwise precisely that seen among hirudiniforms. Linta be may be the ‘‘ Salifa cambouei , [que] est encore inédite; elle habite Madigascar’’ as footnoted by Blanchard (1897c: 102). Now a nomen nudem, the species name remained without a description and no deposited specimens are known.

Barbronia gwalagwalensis , new species

Figures 9–13

DESCRIPTION: One holotype, AMNH Annelida 5261, fixed in 10% buffered formalin and stored in 70% ethanol. One paratype, AMNH Annelida 5262, fixed in 10% buffered formalin and stored in 70% ethanol. Two paratypes, AMNH Annelida 5263, fixed and stored in 95% ethanol. Adults up to approximately 25 mm in length. Body cylindrical, vermiform (fig. 9). Dorsum reddish brown in life, tan or pale gray postfixation. Annulation formula of midbody somites: c1, c2, c3, c4, a2, b5, c11, c12 (fig. 11). Anus dorsal on XXVII, two postanal annuli anterior to caudal sucker. Caudal sucker ventrally directed in relaxed specimens. Eyes: three pairs; first dorsal, on II; second and third pairs dorsolateral on IV, separated from anterior pair by four complete annuli exclusive (fig. 9 inset). Clitellum distinct, from X b5 to XII a2 inclusive (fig. 9). Genital pores separated by seven and one­half annuli (fig. 11). Male gonopore on XII c2. Female gonopore at XIII c1/c2. Two accessory copulatory pits present (fig. 11), one anterior to male gonopore at X/XI, one posterior to female gonopore at XIII/XIV. Ovisacs paired, caecate extending to XIV c4/a2 (fig. 12). No common oviduct. Male atrium extremely small, nearly absent where thin sperm ducts independently reach male gonopore (fig. 13). No common sperm duct. Ejaculatory bulbs on sperm ducts at XIII/XIV (fig. 13). Testisacs 12 in total, six per side between XIV and XV. Pharyngeal stylet pairs on myognaths, small, digitiform, parallel to body axis. Often only stylet pore visible.

TYPE LOCALITY: Maia’s Dam , Gwalagwala a tented­camp near Hoedspruit, South Africa .

ETYMOLOGY: For the type locality of the species: Gwalagwala. Gwalagwala is Zulu for the purple­crested lourie.

REMARKS: The genus Barbronia is defined as having two accessory copulatory pores on X/XI and XIII/XIV even though Moore (1939) described Barbronia delicata without the accessory pores. Sawyer (1986) placed the latter more appropriately into the genus Salifa . With the two copulatory pores and the paired pharyngeal stylets, B. gwalagwalensis clearly falls in the genus Barbronia . The external body patterns of Barbronia gwalagwalensis fall within the range of characteristics reported for B. weberi . However, the most­cited description for B. weberi is from India ( Moore, 1927), and this differs considerably from the type description from Java ( Blanchard, 1897b). For example, B. weberi sensu stricto has three postanal annuli, as clearly figured by Blanchard (1897b: 354); only two are reported by Moore (1927: 139). Barbronia gwalagwalensis is more similar in this regard to the Indian B. weberi sensu lato. However, the Indian specimens show subdivision of annulus a2 into b3 and b4 ( Moore, 1927), whereas B. weberi from the type locality and B. gwalagwalensis do not. In terms of internal anatomy, B. gwalagwalensis has ejaculatory bulbs located on the sperm ducts at XIII/XIV, which is not a characteristic of B. weberi regardless of locality. Rather, the internal male reproductive organs of B. gwalagwalensis closely resemble those of the Egyptian Barbronia assuiti ( Hussein and El­Shimy, 1982; El­Shimy, 1996). All species of Barbronia possess testisacs in two groups of six on either side of the nerve cord but only B. assuiti and B. gwalagwalensis have ejaculatory bulbs on sperm ducts at XIII/XIV ( Hussein and El­Shimy, 1982). Barbronia gwalagwalensis is distinct from B. assuiti in having four annuli between pairs of eyespots, subdivision of b1 and b2, and in the position of the male gonopore. No other species of Barbronia has ovisacs as long as those seen in B. gwalagwalensis . Unpublished research notes of the late Jan Oosthuizen reveal that he had putatively identified a new species of Barbronia from South Africa (AMNH Annelida 5028 through 5037) that he denoted ‘‘latebricola’’. It also possessed ejaculatory bulbs on the sperm ducts, differing from B. gwalagwalensis in that the male gonopore was invariably at the furrow of XII b1/b2. Several anatomical details would support ‘‘latebricola’’ as distinct from B. assuiti as originally described ( Hussein and El­Shimy, 1982); however, after the correction of numerous errors ( El­Shimy, 1996), there is nothing to distinguish ‘‘latebricola’’ from B. assuiti .

Figures

Figs 1–8. Linta be, n.gen., n.sp. 1. Dorsal surface. 2. Anteriolateral view of head showing first four eyespots of left side (arrows). 3. Lateral view of head showing third, fourth, and fifth eyespots of left side. 4. Internal surface of pharynx with 2 of 3 pairs of stylet pores (arrows) on myognaths. 5. Ventral region of clitellum with male (m) and female (f) gonopores. 6. Median reproductive apparatus with paired ovaries including separate short oviducts (od) and irregularly lobate ovisacs (os). 7. Median reproductive apparatus with ovaries removed revealing posteriolaterally directed male atria (a) with an anteriorly glandular region between them. 8. First of five sets of six pairs of testisacs (arrows) of XV/ XVI.

Figs. 9–13. Barbronia gwalagwalensis, n.sp. 9. Dorsal surface with obvious clitellum (c) and typical arrangement of eyespots (inset). 10. Internal surface of pharynx showing one of three pairs of stylet pores (arrows) on a myognath. 11. Ventral region of clitellum with male (m) and female (f) gonopores as well as the accessory copulatory pores (arrows). 12. The right ovary including an oviducts (od) leading to the female gonopore (f) and an irregularly lobate ovisac (os). 13. Median male reproductive apparatus showing the lack of a prominent atrium at the male gonopore (m) where the sperm ducts (sd) meet as well as the paired ejaculatory bulbs (arrows) just posterior to the female gonopore (f).