Phyllocnistis, Zeller, 1848

Kirichenko, Natalia, Triberti, Paolo, Akulov, Evgeniy, Ponomarenko, Margarita, Gorokhova, Svetlana, Sheiko, Viktor, Ohshima, Issei & Lopez-Vaamonde, Carlos, 2019, Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding, Zootaxa 4652 (1), pp. 1-55 : 39

Phyllocnistis sp. 1

( Fig. 14H )

Material examined. Russia: PK, Gornotaezhnoe , forest around MTS, 43.68N, 132.17E, 224 m alt., Salix sp., 23.VII.2016, 1 larva, NK595 , MG 191427 , deposited in INRA.

Leaf mine. The mine is a long relatively slender whitish epidermal tunnel, slightly wavy, usually starting at the leaf tip and proceeding along the main vein, on the lower side of the leaf ( Fig. 14H ). The growing mine increases in width insignificantly. Pupation in the mine, in the cocoon, near the leaf margin on the lower side ( Fig. 14H ).

Trophic specialization. Monophagous on Salix sp. ( Salicaceae ).

Distribution. Russia: RFE—PK.

Remarks. BIN of unknown species—BOLD: ADF2906. Overall, only two Salix -feeding Phyllocnistis are known in East Asia: Ph. gracilistylella Kobayashi, Jinbo et Hirowatari and Ph. saligna (Zeller) ( De Prins & De Prins 2018) . Other three species that develop on Salix , occurs in the western part of the Palearctic: Ph. canariensis Hering , Ph. ramulicola Langmaid et Corley and Ph. valentinensis Hering ( De Prins & De Prins 2018) . The DNA barcode sequence of Phyllocnistis sp. 1 does not match any of these species. The closest neighbor, with 4.9% divergence, is Ph. valentinensis from Austria, followed by Ph. gracilistylella from Japan, 6.4% ( Table 2 ). Other three species are significantly genetically distant from Phyllocnistis sp. 1, i.e.> 12.9 % ( Table 2 ). Phyllocnistis sp. 1 is a putative new species to science. It was mentioned in our recent study ( Kirichenko et al. 2018a).

Figures

FIGURE 14. Mines, leaf shelters and pupation sites of Phyllonorycter, Chrysaster and Cameraria spp. from the Russian Far East. (A) Phyllonorycter sp. 7, host plant Ulmus glabra; (B–C) Phyllonorycter sp. 8, Juglans mandshurica; (D–E) Chrysaster hagicola, Lespedeza bicolor; (F–G) Cameraria niphonica, Acer pseudosieboldianum and A. caudatum subsp. ukurundense, respectively; (H) Phyllocnistis sp. 1, Salix sp. Indications: (m) mine; (b) blotch part of the mine; (t) epidermal tunnel; (l) larva; (p) pupation site. Close up: (C–G) mine; (H) pupation site. Sampling locations: (A) SO, Sakhalin Isl., Yuzhno-Sakhalinsk, 11–20.VII.2017; (B–H) PK, Gornotaezhnoe, MTS and forest, 22–26.VII.2016.